20 Questions
What is the approximate genome size of the helical nucleocapsids with negative sense ssRNA genome?
10-15kb
Where was the first outbreak of Marburg virus?
Marburg and Frankfurt, Germany
Which type of EBOV is considered the most fatal?
Zaire EBOV
What is the probable source of transmission for Marburg and Ebola viruses to primates?
Fruit bats (zoonotic spread)
What is the potential function of sGP (soluble glycoprotein) in Ebola virus infection?
Act as a decoy antigen
What is the role of minor nucleocapsid protein VP30 in Ebola virus infection?
Activates viral mRNA synthesis
Where is VP40, the most abundant viral protein in Ebola virus, located?
Cytoplasmic side of plasma membrane and/or inner side of viral envelope
What does the observation of periodic spikes in EBOV antibodies in survivors suggest?
Latent infection and potential disease recrudescence
Why do researchers use different viruses instead of the specific virus when generating pseudotypes?
Due to safety concerns
What is the proposed function of sGP (soluble glycoprotein) in Ebola virus infection?
Reduce the production of pro-inflammatory cytokines
What is the composition of Ebola virus genome?
Single-stranded RNA
Where does Ebola virus replication occur?
Cytoplasm
What is the lethality rate of Ebola virus?
90%
Which proteins are packaged in the Ebola virus virion?
$NP, VP35, GP, GP1, GP2$
What is the function of Ebola virus glycoprotein (GP)?
$Mediates attachment and entry into host cells by fusion$
What is the role of mRNA editing in Ebola virus?
$To produce additional secreted glycoproteins (sGP, delta-peptide)$
What cellular receptors does Ebola virus glycoprotein (GP) bind to?
$Folate receptor-alpha$
What type of infections are Filovirus infections among humans limited to?
$Close contacts, such as family members and medical staff$
What is contained in the inclusion bodies in the cytoplasm of infected cells?
$Viral nucleocapsid$
What is used to study which viral proteins bind to which cellular receptors?
$Proteomics$
Study Notes
Ebola Virus Replication and Glycoprotein Synthesis
- Ebola virus lethality can be as high as 90%
- Filovirus infections among humans are limited to close contacts, such as family members and medical staff
- Ebola virus has a linear, negative sense, single-stranded RNA genome with seven genes
- Most Ebola virus proteins are packaged in the virion, including nucleocapsid protein (NP), RNA polymerase cofactor (VP35), and envelope glycoproteins (GP, GP1, GP2)
- Ebola virus makes additional secreted glycoproteins (sGP, delta-peptide)
- Ebola virus has mRNA editing, whereas Marburg virus does not
- Ebola virus replication occurs in the cytoplasm and involves the synthesis of antigenome RNA
- The cytoplasm of infected cells contains inclusion bodies containing viral nucleocapsid
- Ebola virus uses RNA editing to make two glycoproteins from the same gene: sGP and GP
- Ebola virus glycoprotein (GP) mediates attachment and entry into host cells by fusion
- GP mediates binding to multiple cellular receptors, including asialoglycoprotein receptor, folate receptor-alpha, integrin, and DC-Signs
- Replication-deficient pseudotypes are used to study which viral proteins bind to which cellular receptors
Test your knowledge of Marburg virus characteristics and outbreaks with this quiz covering filum, filamentous particles, genome size, and historical outbreaks in Germany, Egypt, and Sudan.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
