Podcast
Questions and Answers
Explain the difference between the lytic and lysogenic cycles in bacteriophages.
Explain the difference between the lytic and lysogenic cycles in bacteriophages.
The lytic cycle involves immediate replication and lysis of the host cell, while the lysogenic cycle involves integration of the phage DNA into the host genome, remaining dormant for many cell divisions before transitioning to the lytic cycle.
What is a prophage and how does it contribute to lysogenic infection?
What is a prophage and how does it contribute to lysogenic infection?
A prophage is the integrated form of phage DNA within the host genome. It remains quiescent and allows the host bacterium to function normally until it is eventually released and reverts to the lytic mode.
What role do Cos sites play in the lysogenic cycle of bacteriophages?
What role do Cos sites play in the lysogenic cycle of bacteriophages?
Cos sites allow the linear DNA molecule injected into the host cell to be circularized, which is necessary for insertion into the bacterial genome.
Explain the function of the λ packaging system in the lysogenic cycle.
Explain the function of the λ packaging system in the lysogenic cycle.
Signup and view all the answers
How are replacement vectors different from insertion vectors in cloning experiments?
How are replacement vectors different from insertion vectors in cloning experiments?
Signup and view all the answers
