Lysogenic Phages Quiz

Play an AI-generated podcast conversation about this lesson

Explain the difference between the lytic and lysogenic cycles in bacteriophages.

The lytic cycle involves immediate replication and lysis of the host cell, while the lysogenic cycle involves integration of the phage DNA into the host genome, remaining dormant for many cell divisions before transitioning to the lytic cycle.

What is a prophage and how does it contribute to lysogenic infection?

A prophage is the integrated form of phage DNA within the host genome. It remains quiescent and allows the host bacterium to function normally until it is eventually released and reverts to the lytic mode.

What role do Cos sites play in the lysogenic cycle of bacteriophages?

Cos sites allow the linear DNA molecule injected into the host cell to be circularized, which is necessary for insertion into the bacterial genome.

Explain the function of the λ packaging system in the lysogenic cycle.

<p>The λ packaging system assembles mature phage particles and can only insert DNA molecules of specific sizes (between 37 and 52 kb) into the phage head structure.</p> Signup and view all the answers

How are replacement vectors different from insertion vectors in cloning experiments?

<p>Replacement vectors have two recognition sites for the cloning restriction endonuclease, flank a segment of replaceable DNA, and are designed to carry larger DNA fragments compared to insertion vectors.</p> Signup and view all the answers