Liquid Chromatography Techniques

The primary difference is the pressure used, with HPLC using high pressure (up to 6000 psi) and LPLC using lower pressure.

Ion exchange chromatography separates ions and molecules based on their charge, involving the exchange of ions between the stationary phase and the mobile phase.

The main advantage of TLC is that it is fast, inexpensive, and simple to perform, and can be used for qualitative and quantitative analysis.

<p>Affinity chromatography separates molecules based on their specific binding interactions, involving the use of a ligand that is attached to the stationary phase and binds specifically to the target molecule.</p> Signup and view all the answers

<p>The primary application of HPLC is in pharmaceutical analysis, biomedical research, and food and beverage analysis.</p> Signup and view all the answers

<p>Cation exchange chromatography separates positively charged ions, while anion exchange chromatography separates negatively charged ions.</p> Signup and view all the answers

<p>In normal phase LC, the stationary phase is polar and interacts with the polar sample molecules, while the non-polar mobile phase carries the sample through the column. In reversed phase LC, the stationary phase is non-polar and interacts with the non-polar sample molecules, while the polar mobile phase carries the sample through the column.</p> Signup and view all the answers

<p>The high pressure in HPLC forces the mobile phase through the column more quickly, resulting in faster analysis times, higher resolution, and improved sensitivity due to the reduction in peak broadening.</p> Signup and view all the answers

<p>The solvent in TLC acts as the mobile phase, moving up the plate and carrying the sample components with it. The solvent's properties, such as its polarity and flow rate, influence the separation of the sample components, with more polar compounds migrating farther up the plate.</p> Signup and view all the answers

<p>The charged groups on the stationary phase resin or gel in IEC facilitate the exchange of ions between the stationary phase and the mobile phase, allowing for high-resolution separations of anions and cations. The charged groups attract ions of opposite charge, enabling selective retention and separation.</p> Signup and view all the answers

<p>AC's use of specific interactions between the stationary phase and sample molecules provides high specificity and selectivity, enabling purification and analysis of biomolecules with high accuracy. This is in contrast to other techniques, which may rely on nonspecific interactions or physical properties.</p> Signup and view all the answers

<p>HPLC provides high-resolution separations and sensitive detection, TLC enables quick and easy identification of compounds, and IEC offers high-resolution separations of ions. Together, these techniques enable comprehensive analysis and quality control of pharmaceuticals, ensuring their purity and efficacy.</p> Signup and view all the answers