Lipid Metabolism and Energy Sources
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Questions and Answers

How many NADH molecules are formed during the β oxidation of Palmitoyl-CoA?

  • 8
  • 10
  • 5
  • 7 (correct)
  • What is the total yield of ATP from one molecule of Palmitoyl-CoA oxidation?

  • 120 ATP
  • 108 ATP (correct)
  • 92 ATP
  • 95 ATP
  • Which enzyme produces FADH2 during the oxidation of Palmitoyl-CoA?

  • Isocitrate dehydrogenase
  • Acyl-CoA dehydrogenase (correct)
  • Succinyl-CoA synthetase
  • Malate dehydrogenase
  • Considering the ATP yield from NADH and FADH2, how much ATP is generated from 7 FADH2 molecules?

    <p>10.5 ATP (A)</p> Signup and view all the answers

    What is the number of ATP produced from the isocitrate dehydrogenase enzyme during the citric acid cycle?

    <p>20 ATP (C)</p> Signup and view all the answers

    What effect does PKA have on glycogen synthase?

    <p>Inhibits glycogen synthesis (B)</p> Signup and view all the answers

    What is the role of glycerol kinase in fat metabolism?

    <p>Phosphorylates glycerol using ATP (B)</p> Signup and view all the answers

    How does fatty acyl-CoA form from fatty acids?

    <p>Via the addition of CoA and ATP (C)</p> Signup and view all the answers

    What happens to fatty acids shorter than 12 carbons in the mitochondria?

    <p>They can diffuse freely across membranes (B)</p> Signup and view all the answers

    Which process occurs after the conversion of glycerol to glycerol-3-phosphate?

    <p>Glycerol is phosphorylated using ATP (B)</p> Signup and view all the answers

    What stimulates the conversion of fatty acyl-CoA back to fatty acids inside the mitochondria?

    <p>Activation of carnitine acyltransferase 1 (A)</p> Signup and view all the answers

    What is the effect of PKA on glycolysis?

    <p>Inhibits glycolysis by inhibiting pyruvate kinase (B)</p> Signup and view all the answers

    Which transporter aids in the mitochondrial import of fatty acids?

    <p>Carnitine transporter (D)</p> Signup and view all the answers

    What is the primary product generated during the oxidative conversion of fatty acids to acetyl-CoA in Stage 1 of fatty acid oxidation?

    <p>Acetyl-CoA (B)</p> Signup and view all the answers

    What is the primary advantage of fats over polysaccharides as an energy source?

    <p>Fats carry more energy per carbon and contain less water. (A)</p> Signup and view all the answers

    Which enzyme is responsible for the dehydrogenation step in the β-oxidation pathway?

    <p>Acyl-CoA dehydrogenase (C)</p> Signup and view all the answers

    Which system do chylomicrons enter before draining into large veins?

    <p>Intestinal lymphatic system (C)</p> Signup and view all the answers

    What is the net result of the thiolysis reaction in Stage 4 of fatty acid oxidation?

    <p>Release of acetyl-CoA (D)</p> Signup and view all the answers

    How does the oxidation of unsaturated fatty acids differ from saturated fatty acids in terms of FADH2 production?

    <p>It produces 1 fewer FADH2 (A)</p> Signup and view all the answers

    How are fatty acids primarily carried in the blood after being released from adipocytes?

    <p>Bound to serum albumin (A)</p> Signup and view all the answers

    What percentage of energy needs does oxidation of fatty acids meet for the mammalian heart and liver?

    <p>About 80% (B)</p> Signup and view all the answers

    What role does NAD play in the β-oxidation pathway?

    <p>Acts as a hydride acceptor (B)</p> Signup and view all the answers

    What role do medium chain triglycerides (MCTs) play in drug delivery?

    <p>They allow for transport through chylomicrons, avoiding hepatic metabolism. (C)</p> Signup and view all the answers

    Which isoform of acyl-CoA dehydrogenase is involved in the oxidation of fatty acids with 12–18 carbons?

    <p>Very-long-chain acyl-CoA dehydrogenase (D)</p> Signup and view all the answers

    What does MAG lipase (MGL) specifically hydrolyze?

    <p>Monoglycerides to fatty acids and glycerol (D)</p> Signup and view all the answers

    In the β-oxidation pathway, what does the step involving enoyl-CoA hydratase produce?

    <p>An alcohol on the β carbon (A)</p> Signup and view all the answers

    Which molecule is formed from the overall process of fatty acid oxidation via the citric acid cycle?

    <p>NADH and FADH2 (D)</p> Signup and view all the answers

    Which type of animals primarily rely on stored fatty acids for energy during hibernation?

    <p>Land mammals (C)</p> Signup and view all the answers

    Which transport mechanism do fatty acids utilize to enter myocytes after leaving serum albumin?

    <p>Active transport via specific transporters (B)</p> Signup and view all the answers

    Flashcards

    Lipid Metabolism

    The process of digesting, mobilizing, and oxidizing fats (lipids) and synthesizing them from other molecules for energy production and other biological functions.

    Fatty Acid Oxidation

    A major energy source, crucial for organs like the heart and liver, obtaining energy from fatty acids.

    Chylomicrons

    Packages for transporting hydrophobic lipid molecules in the bloodstream.

    Fatty Acid Mobilization

    The process of releasing stored fats for use as an energy source.

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    Fat Storage Efficiency

    Fats store more energy per unit of weight compared to carbohydrates.

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    Apolipoproteins

    Proteins that package hydrophobic lipids for transport in the blood.

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    Myocyte Fatty Acid Oxidation

    Fatty acids are oxidized within muscle cells to produce ATP for muscle contraction and other metabolic processes.

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    Dietary Triacylglycerol (TAG)

    A major source of dietary fat used for energy in the body.

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    PKA's effect on glycogen metabolism

    PKA inhibits glycogen synthase, preventing glycogen creation, and activates glycogen phosphorylase kinase, promoting glycogen breakdown into glucose.

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    PKA's effect on Glycolysis

    PKA inhibits glycolysis by inhibiting PFK-2 and pyruvate kinase, reducing glucose usage.

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    Glycerol's entry into glycolysis

    Glycerol is phosphorylated by glycerol kinase to enter glycolysis, generating energy from fats.

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    Fatty acid transport to mitochondria

    Fatty acids are converted to fatty acyl-CoA for transport into the mitochondria for oxidation.

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    Fatty Acyl-CoA formation

    Fatty acids convert to fatty acyl-CoA using fatty acyl-CoA synthetase via ATP hydrolysis, releasing AMP and PPi.

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    Fatty acid transport (large)

    Large fatty acids are transported into the mitochondria via acyl-carnitine/carnitine transporter.

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    Fatty acid transport into mitochondria (small)

    Small fatty acids (<12 carbons) directly cross mitochondrial membranes and don't need a transporter.

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    Acyl-carnitine/carnitine transporter

    A transporter that aids fatty acid entry into the mitochondria. It is a crucial part of that process involving fatty acids.

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    β-Oxidation

    The process of breaking down fatty acids into acetyl-CoA, generating NADH and FADH2 for ATP production.

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    Acyl-CoA dehydrogenase

    An enzyme involved in the β-oxidation pathway that catalyzes the first step, creating FADH2.

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    β-Hydroxyacyl-CoA dehydrogenase

    An enzyme in the β-oxidation pathway oxidizing a hydroxyl group, generating NADH.

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    NADH and FADH2 in ATP production

    NADH and FADH2 act as electron carriers in the electron transport chain, ultimately driving ATP synthesis.

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    How many ATP from Palmitoyl-CoA oxidation?

    The complete oxidation of palmitoyl-CoA yields approximately 108 ATP molecules.

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    Fatty Acid Oxidation Stages

    The breakdown of fatty acids into acetyl-CoA, occurring in three stages in the mitochondria: oxidative conversion to acetyl-CoA, oxidation of acetyl-CoA, and ATP production from NADH/FADH2 via oxidative phosphorylation

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    Beta-oxidation

    The process of removing two-carbon acetyl-CoA units from a fatty acid chain, generating NADH and FADH2

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    Enoyl-CoA Hydratase

    Enzyme that hydrates the double bond created in the previous step.

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    Beta-hydroxyacyl-CoA dehydrogenase

    Enzyme that oxidizes the alcohol group, producing another NADH molecule.

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    Acyl-CoA acetyltransferase (thiolase)

    Enzyme that cleaves the fatty acyl chain, releasing acetyl-CoA.

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    Unsaturated Fatty Acid Oxidation

    Oxidation of fatty acids with double bonds, producing one less FADH2 molecule in the initial cycle.

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    Fatty Acid Oxidation Location

    Fatty acid oxidation occurs in the mitochondria. The electron transport chain is also present in the inner mitochondrial membrane (IMM).

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    Study Notes

    Lipid Metabolism

    • One-third of energy needs come from dietary triacylglycerols (TAGs).
    • Fatty acid oxidation is a major energy source.
    • 80% of mammalian heart and liver energy needs are met by fatty acid oxidation.

    Hibernating Animals and Polar Bears

    • Hibernating animals and land-locked polar bears get most of their energy from stored fatty acids.

    Fats vs. Polysaccharides

    • Fats carry more energy per carbon because they are more reduced.
    • Fats carry less water because they are nonpolar.
    • Glucose and glycogen are for short-term energy needs with rapid delivery.
    • Fats are for long-term energy needs, good storage, and slow delivery over months.

    Mobilization of Dietary Fats

    • Dietary fats are packaged into chylomicrons in the small intestine.
    • Bile salts emulsify dietary fats, forming mixed micelles.
    • Intestinal lipases degrade triacylglycerols.
    • Fatty acids and other breakdown products are incorporated into triacylglycerols, then into chylomicrons with cholesterol and apolipoproteins.
    • Chylomicrons move through lymphatic system and bloodstream to tissues.
    • Lipoprotein lipase, activated by apoC-ll, converts triacylglycerols to fatty acids and glycerol.

    Lipids and Transport

    • Lipids are hydrophobic and need packaging for transport in the blood.
    • Chylomicrons are formed with triacylglycerols, cholesteryl esters, cholesterol, and phospholipids, along with apolipoproteins.
    • Chylomicrons are released into the intestinal lymphatic system, then into large veins, and delivered throughout the body.

    Avoiding Hepatic Metabolism

    • Small lipophilic drugs packaged with long chain triglycerides can be incorporated into chylomicrons and carried to the lymph, then bloodstream, avoiding the liver.
    • MCT = medium chain triglycerides
    • GML = glyceryl monolinoleate (a long chain triglyceride)
    • SEDDS = self-emulsifying formula made with GML

    Lipoproteins

    • Hydrophobic lipids are packaged with apolipoproteins in the blood for transport.
    • Chylomicrons (density < 0.95 gm/ml): very large, carry lipid from intestine to periphery (100-1000 nm).
    • VLDL (density 0.95-1.0 gm/ml): large, carry lipid from liver to periphery.
    • LDL (density 1.00-1.06 gm/ml): carry cholesterol to periphery.
    • HDL (density 1.06-1.2 gm/ml): smallest, carries cholesterol back to the liver.

    Lipases and Glycerol

    • Lipases cleave fatty acids from glycerol in the backbone of triacylglycerols.
    • Glycerol kinase phosphorylates glycerol using ATP.
    • Glycerol enters glycolysis after phosphorylation.

    Intracellular Transport

    • Fatty acids are converted to fatty acyl-CoA for intracellular transport.
    • Fatty acid + CoASH + ATP fatty acyl-CoA + AMP + PPi
    • The reaction is catalyzed by fatty acyl-CoA synthetase.

    Fatty Acid Oxidation and Mitochondria

    • Fats and triglycerides are decomposed to fatty acids and glycerol in the cytoplasm of adipocytes.
    • Fatty acids are transported to other tissues via the blood.
    • Fatty acids are oxidized in mitochondria.
    • Small fatty acids (less than 12 carbons) diffuse across mitochondrial membranes freely.
    • Large fatty acids use a carnitine/acyl-carnitine transporter.

    Acyl-Carnitine/Carnitine Transporter

    • Carnitine acyltransferase 1 attaches fatty acid to carnitine, forming fatty acyl carnitine.
    • Fatty acyl carnitine diffuses across the outer mitochondrial membrane (OMM).
    • Fatty acyl carnitine crosses into the matrix by counter-exchange with carnitine.
    • Carnitine acyltransferase 2 reattaches fatty acid to CoA.

    Fatty Acid Oxidation Stages

    • Stage 1: Oxidative conversion of 2-carbon units to acetyl-CoA via β-oxidation, generating NADH and FADH2.
    • Stage 2: Oxidation of acetyl-CoA into CO2 via the citric acid cycle, producing NADH and FADH2.
    • Stage 3: ATP production from NADH & FADH2 via oxidative phosphorylation.

    β-Oxidation Pathway

    • Removal of acetyl groups in the form of acetyl-CoA.
    • Steps of oxidation, hydration, oxidation, and thiolysis are involved.

    Oxidation of Unsaturated Fatty Acids

    • Results in one fewer FADH2, after isomerization, and 1 FADH2 is produced during the first step of the next cycle.
    • NADPH reduces the remaining unsaturated bond, making sure there's no further loss of FADH2.

    NADH and FADH2 in ATP Production

    • NADH and FADH2 are used as sources of ATP.
    • Specific enzymes catalyze the oxidation steps in β-oxidation.
    • The final amount of ATP produced varies, based on the steps in the process.

    Hormone Regulation

    • Glucagon and epinephrine activate lipases, triggering the mobilization of stored triacylglycerols when blood glucose is low.
    • PKA phosphorylates hormone-sensitive lipase (HSL) causing perilipin to dissociate and exposing stored triacylglycerols.
    • ATGL converts triacylglycerol (TAG) to diacylglycerol (DAG).
    • P-HSL converts DAG to monoacylglycerol (MAG).
    • MAG lipase (MGL) converts monoacylglycerol (MAG) to glycerol and fatty acids.
    • Fatty acids enter myocytes and undergo oxidation via the citric acid cycle for ATP production.

    Glycogen Metabolism and PKA

    • PKA regulates multiple pathways to maintain glucose homeostasis, including inhibition of glycogen synthase, activation of glycogen phosphorylase kinase (glycogen breakdown).
    • PKA inhibits glycolysis, including pyruvate kinase.
    • PKA activates fatty acid metabolism, including hormone-sensitive lipase, so the body uses fat as an energy source.

    Glycerol in Glycolysis

    • Glycerol kinase phosphorylates glycerol.
    • After oxidation, the phosphorylated glycerol molecule enters glycolysis.
    • Glycerol entry into glycolysis allows for limited anaerobic catabolism of fats.

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    Description

    This quiz explores the intricacies of lipid metabolism, examining how dietary fats are utilized for energy in both mammals and hibernating animals. It highlights the differences between fats and polysaccharides in energy storage and delivery, emphasizing the significance of fatty acid oxidation. Test your understanding of these concepts and their implications for energy needs.

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