Podcast
Questions and Answers
What is decontamination?
What is decontamination?
The process of removal of contaminating pathogenic microorganisms from articles by sterilization or disinfection.
Define asepsis.
Define asepsis.
The use of techniques to achieve a microbe-free environment, such as gloves, air filters, and UV rays.
Why is it important to regard all chemicals as toxic unless proven otherwise?
Why is it important to regard all chemicals as toxic unless proven otherwise?
To prevent potential risks associated with toxicity, chemical burns, dermatitis, explosions, and fires.
What are the main dangers associated with chemical hazards in laboratories?
What are the main dangers associated with chemical hazards in laboratories?
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What is the risk associated with benzene vapors in laboratories?
What is the risk associated with benzene vapors in laboratories?
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Why are strong acids or alkalis (bases) hazardous in laboratories?
Why are strong acids or alkalis (bases) hazardous in laboratories?
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Name two highly poisonous solid substances mentioned in the text.
Name two highly poisonous solid substances mentioned in the text.
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What is the biggest cause of lab accidents according to the text?
What is the biggest cause of lab accidents according to the text?
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How can one prevent injuries from glass hazards in laboratories?
How can one prevent injuries from glass hazards in laboratories?
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Why is it important to have safety rules in place in laboratories?
Why is it important to have safety rules in place in laboratories?
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Study Notes
Laboratory Specialties
- Hematology: Study of blood and its components.
- Clinical Chemistry: Analysis of bodily fluids for diagnostic purposes.
- Urinalysis: Examination of urine for medical conditions.
- Immunology: Study of the immune system and its responses.
- Serology: Analysis of serum, typically for antibodies and antigens.
- Histopathology: Microscopic examination of tissue to study disease.
- Immunohematology: Study of blood group antigens and antibodies.
- Molecular Biology: Focus on the molecular mechanisms within living organisms.
General Safety Rules
- Follow instructions meticulously before conducting lab work.
- Use safety goggles to guard against chemical splashes and projectiles.
- Report spills or accidents immediately to a supervisor.
- Wash hands thoroughly with soap and water after handling chemicals.
- Keep hands away from the face during lab activities to prevent contamination.
- Secure long hair to avoid entanglement in equipment.
- Familiarize yourself with emergency equipment locations: fire extinguisher, fire blanket, eyewash station, and first aid kit.
- Maintain a tidy workspace and only bring essential items to the lab.
- Never consume food or drink in the laboratory setting.
- Clean the lab workspace after experiments to promote safety and hygiene.
Sterilization and Disinfection
- Sterilization: Complete destruction of all microorganisms, including spores; achievable through physical, chemical, or physiochemical methods.
- Disinfection: Reduces pathogenic microorganisms on surfaces but does not eliminate spores; accomplished via physical or chemical strategies.
- Disinfectants vary in effectiveness and target ranges; not all disinfectants are capable of eliminating all microbes.
- Contaminants such as stool or pus can result in severe infections; proper disposal of broken glass is crucial.
- Reagent bottles must be returned to shelves immediately after use, and all containers should be clearly labeled.
Laboratory Hazards and Accidents
- Common laboratory mechanical hazards include equipment such as centrifuges and shakers.
- Main hazards associated with lab work are:
- Infection from pathogens.
- Burns from flammable chemicals.
- Cuts from sharp objects.
- Harmful effects from toxic substances.
- Injuries from explosions.
- Risks of electric shock and fire.
Infection Prevention
- Pathogens can be transmitted by:
- Inhaling aerosols while closing specimen containers or pipetting.
- Contaminated fingers or food from the lab fridge.
- Mouth-pipetting, a major route for pathogen ingestion.
- Skin punctures or cuts from infected needles or insect bites.
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Description
Test your knowledge on laboratory safety rules and clinical chemistry with this quiz. Questions cover topics such as handling chemicals safely, wearing protective gear, and following instructions in a lab setting.
