Clinical Chemistry Post Lecture Exam
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Questions and Answers

What is the preferred analytical method used in dry slide technology for measuring analytes?

  • Fluorometry
  • Reflectance photometry (correct)
  • Nephelometry
  • PCR
  • What is the primary purpose of passing water through resin with charged particles?

  • To produce distilled water
  • To create uncharged water
  • To achieve deionized water (correct)
  • To make resinous water
  • What method dissociates elements using heat energy instead of exciting electrons?

  • Fluorometry
  • Atomic Absorption Spectrophotometry
  • Flame Photometry (correct)
  • Nephelometry
  • What is captured by spectrophotometry for measurement?

    <p>Electromagnetic radiation</p> Signup and view all the answers

    What term refers to the degree of correspondence between the obtained value and the true value?

    <p>Accuracy</p> Signup and view all the answers

    Which equipment does not require calibration?

    <p>Microscope</p> Signup and view all the answers

    What causes interference in atomic absorption spectrophotometry due to differences in viscosity?

    <p>Ionization interference</p> Signup and view all the answers

    Convert 10 mg/dL of immunoglobulins to g/L.

    <p>0.01 g/L</p> Signup and view all the answers

    All of the following are possible causes of random error, EXCEPT:

    <p>Deterioration of reagents</p> Signup and view all the answers

    What is the gradual change in the control sample results called?

    <p>Trend</p> Signup and view all the answers

    Which Westgard rule is typically used as a warning?

    <p>1 2s</p> Signup and view all the answers

    What is the term for the quality management whereby patient's results are checked against their previous records?

    <p>Delta-check</p> Signup and view all the answers

    The acronym RACE for fire safety includes an E. What does it stand for?

    <p>Evacuate</p> Signup and view all the answers

    The ability of an analytical method to measure only the analyte of interest is called?

    <p>Specificity</p> Signup and view all the answers

    What is considered important in maintaining the long-term accuracy of analytical methods?

    <p>Quality assurance</p> Signup and view all the answers

    Which hormone is primarily responsible for decreasing glucose levels?

    <p>Insulin</p> Signup and view all the answers

    What value indicates impaired glucose tolerance after a 2-hour OGTT?

    <p>123 mg/dL</p> Signup and view all the answers

    Which of the following hormones tends to increase glucose concentration?

    <p>Cortisol</p> Signup and view all the answers

    Which test strongly differentiates type 1 diabetes from type 2 diabetes?

    <p>C-peptide</p> Signup and view all the answers

    Gestational diabetes commonly develops into full-blown diabetes within:

    <p>5 to 10 years</p> Signup and view all the answers

    What is the required glucose load for pregnant patients during a glucose challenge test?

    <p>75 grams</p> Signup and view all the answers

    C-peptide concentration is decreased or undetectable in which condition?

    <p>Type 1 DM</p> Signup and view all the answers

    A positive test for glucose in urine suggests that the blood glucose level is above:

    <p>200 mg/dL</p> Signup and view all the answers

    Total glycosylated hemoglobin levels in a hemolysate reflect the average blood glucose levels for which time period?

    <p>Past 2-3 months</p> Signup and view all the answers

    What does the hexokinase method of glucose measurement assess?

    <p>Absorbance of NADPH at 340 nm</p> Signup and view all the answers

    For asymptomatic diabetes, which conditions are commonly checked?

    <p>All three mentioned</p> Signup and view all the answers

    Which glucose monitoring test can best determine Boots' compliance with blood sugar medications?

    <p>Glycated albumin</p> Signup and view all the answers

    Which test is best for measuring glucose homeostasis?

    <p>Oral glucose tolerance test (OGTT)</p> Signup and view all the answers

    What is the term for the ability to maintain a steady glucose concentration in the blood?

    <p>Glucoregulation</p> Signup and view all the answers

    Which enzyme can be added in the glucose oxidase method to help convert alpha-D-glucose to beta-D-glucose?

    <p>Mutarotase</p> Signup and view all the answers

    What hormone, produced by the alpha-cells of the pancreas, regulates glucose levels?

    <p>Glucagon</p> Signup and view all the answers

    Which statistical test is used to determine if there is a significant difference between the means of two groups?

    <p>T test</p> Signup and view all the answers

    Identify the type of chromatography that employs pressure for expedited separations and incorporates controlled temperature and gradient elution techniques.

    <p>High Performance Liquid Chromatography</p> Signup and view all the answers

    Which sequence accurately reflects the pathway of light in a single beam spectrophotometer?

    <p>Light source -&gt; Entrance slit -&gt; Monochromator -&gt; Cuvet -&gt; Exit Slit -&gt; Photodetector -&gt; Read out device</p> Signup and view all the answers

    What type of monochromator uses wedged glass pieces that can be rotated to allow only specific wavelengths to pass through?

    <p>Prisms</p> Signup and view all the answers

    Which photodetector is primarily used for measuring the visible and UV regions of light?

    <p>Photomultiplier tube</p> Signup and view all the answers

    What technique determines the amount of light blocked by particulate matter in a turbid solution?

    <p>Turbidimetry</p> Signup and view all the answers

    What is the purpose of a holmium oxide glass filter?

    <p>To assess wavelength accuracy</p> Signup and view all the answers

    Which anticoagulant is preferred for blood gas studies?

    <p>Lithium heparin, 100 U/mL blood</p> Signup and view all the answers

    What is the inflow velocity of air in a Class II B1 biosafety cabinet?

    <p>100 FPM with 30% recirculated air</p> Signup and view all the answers

    Which characteristic is NOT essential for an ideal quality control material?

    <p>Exhibits known matrix effects</p> Signup and view all the answers

    What is the coefficient of variation when the mean is 120 mg/dL and 2SD is 6.0?

    <p>5%</p> Signup and view all the answers

    How do you convert 5 g/dL of total protein to g/L?

    <p>50 g/L</p> Signup and view all the answers

    Which wavelength would be most strongly absorbed by a red-colored solution?

    <p>650 nm</p> Signup and view all the answers

    According to Beer's law, how is absorbance related to concentration?

    <p>Absorbance increases as concentration increases</p> Signup and view all the answers

    What is the representation of the Beer-Lambert law linking absorbance and transmittance?

    <p>A = -log(%T)</p> Signup and view all the answers

    Study Notes

    Clinical Chemistry Post Lecture Exam - Study Notes

    • RACE acronym: In fire safety, the E stands for Extinguish.

    • Delta Check: The difference between two consecutive measurements of the same individual.

    • Westgard Rules: The 2 2s rule is used as a warning.

    • Quality Management: Benchmarking is the process of comparing a patient's results against previous records.

    • Analytical Methods: Accuracy is the ability of a method to measure the analyte of interest. Precision describes the ability of measurements to be consistent. Specificity ensures only the analyte of interest is measured, while sensitivity measures the method's ability to detect small amounts of the analyte.

    • Quality Control: Maintaining the accuracy of analytical methods is a key aspect of quality control. This typically involves quality assurance procedures and interlaboratory quality control.

    • Fire Safety/Precautions: The process of handling samples, reagents, and equipment in a laboratory setting is paramount to health and safety. Proper precautions and safety protocols are crucial in minimizing risks.

    • Clinical Chemistry Phases: Pre-analytic, analytic, post-analytic phases are involved in lab testing.

    • Random Error Cause: Possible causes include pipetting error, deterioration of reagents, and temperature fluctuations.

    • Systematic Error Cause: Possible causes include contaminated solutions, leaky ISEs, improper mixing of reagents and samples, unstable reagent blanks.

    • Significant Figures/Data Reporting: Correct pipetting using air displacement ensures accurate measurements during lab testing procedures.

    • Conversion: 10 mg/dL of immunoglobulins = 1 g/L.

    • Extreme Danger: The numerical classifications for extreme danger vary depending on the specific context and are not consistently defined.

    • Lean Six Sigma Quality Improvement: Procedures involved include Define, Measure, Analyze, Improve, and Control (DMAIC)

    Additional Topics

    • Fire Safety Acronym: The acronym RACE in fire safety is used for Exercise caution, Extinguish, Alert and Evacuate.

    • Instrument Calibration: This is extremely important and must be checked regularly in the laboratory setting.

    • Biohazard Label: The standard biohazard label color for containers used in a clinical lab for specimens or materials with potential risk is Fluorescent yellow.

    • Types of Clinical Chemistry Analyzers: Open/closed reagents testing, sequential, batch testing analyzers.

    • Quality Control in the Laboratory: Quality assurance.

    • Laboratory Testing Procedures: Detailed procedures for handling specimens and performing tests are crucial to accurate diagnoses.

    • Units of Measurement: The appropriate and most accurate unit of measurement is critical in reporting patient results or evaluating lab testing data.

    • Clinical Significance: Understanding relationships between test results and patient diagnoses is essential to appropriate care procedures.

    • Chemical Hazard/Precautionary Measures: Proper precautions to be taken when handling and working with chemical substances are critical, and should be referred to in safety manuals.

    • Fire safety/Precautions: The process of handling samples, reagents, and equipment in a laboratory setting is paramount to health and safety. Proper precautions and safety protocols are crucial in minimizing risks.

    • Quality Control procedures: Accuracy and precision of analysis are critical, and regular quality control measurements should be performed.

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    Description

    Test your knowledge on clinical chemistry with this post-lecture exam. Covering essential topics like quality management, analytical methods, and fire safety measures, this quiz is perfect for students looking to reinforce their understanding. Prepare to delve into the crucial concepts that ensure accuracy and precision in laboratory settings.

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