Laboratory Safety and Scientific Tools

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Questions and Answers

Which of the following practices is essential for proper biohazard waste disposal in a laboratory setting?

  • Leaving it on the lab bench for appropriate personnel to handle
  • Flushing it down the sink to ensure immediate removal
  • Placing it in a standard trashcan for regular disposal
  • Disposing of it in a specially marked bin designated for biohazards (correct)

Why are SI units important in scientific measurements?

  • They vary from country to country
  • They are based on traditional US customary units
  • They provide a standard system of units for international use (correct)
  • They simplify calculations by using fractions instead of decimals

A student measures the length of a leaf multiple times and obtains the following measurements: 15.2 cm, 15.5 cm, 15.1 cm, and 15.4 cm. What is the average length of the leaf?

  • 15.3 cm (correct)
  • 15.2 cm
  • 15.1 cm
  • 15.5 cm

Which instrument would be most appropriate for measuring the diameter of a small pea?

<p>Micrometer (A)</p> Signup and view all the answers

What does the diameter at breast height (DBH) measure?

<p>Tree diameter (C)</p> Signup and view all the answers

In scientific experimentation, what is the primary role of the control group?

<p>To serve as a baseline for comparison without the experimental treatment (B)</p> Signup and view all the answers

A researcher is studying the effect of different concentrations of fertilizer on plant growth. What is the independent variable in this experiment?

<p>Concentration of fertilizer (B)</p> Signup and view all the answers

Which type of graph is most suitable for displaying continuous data, such as temperature changes over time?

<p>Line graph (C)</p> Signup and view all the answers

Which section of a scientific paper provides a brief overview of the study, including the main findings and conclusions?

<p>Abstract (A)</p> Signup and view all the answers

How does allelopathy affect neighboring plants?

<p>By releasing harmful chemicals that inhibit growth (D)</p> Signup and view all the answers

Flashcards

What are SI units?

A standard system of units used to convert between metric and US customary units; also known as the International System of Units.

How to calculate means/averages?

Add all the numbers in the data set, then divide by the number of numbers.

What is Length?

Linear measurement of an object. Basic unit is the meter. Includes length, width, distance, and diameter.

What is Volume?

The space occupied by a liquid or an object. Basic unit is the Liter.

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What is the meniscus?

A curve at the surface of a liquid in a container. Read at eye level, at the bottom of the curve, using a meniscus card.

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What is Temperature?

How hot or cold something is. Measured in Celsius.

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What is DBH?

A standard measure of tree diameter, calculated by dividing the circumference of the tree by pi (approximately 3.14).

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What is Scientific Investigation?

The process of the scientific method to ask or investigate a question.

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Types of variables?

Independent (manipulated), dependent (observed), and controlled (kept constant).

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Replication and sample size?

The ability of an experiment to be replicated. A larger sample size increases accuracy.

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Study Notes

  • Laboratory rules, regulations, and procedures include proper attire, eating and drinking guidelines, and knowledge of the location of Laboratory Emergency Equipment.
  • Wear a sleeved shirt and bottoms that cover your legs in the lab.
  • Open-toed shoes, eating, or drinking are not allowed, especially when dealing with harmful chemicals.
  • Dispose of biohazard waste in a specially marked bin, and never in a trashcan or down the sink.

Scientific Tools and Techniques

  • SI units are a standard system of units called the International System of Units, used to convert between metric and US customary units.
  • Calculate means or averages by adding all the numbers in a data set and dividing by the number of numbers.
  • Length represents the linear measurement of an object, with the meter as its basic unit.
  • Linear measurements include length, width, distance, and diameter; instruments include rulers, yardsticks, micrometers, and measuring tapes.
  • Mass is the weight of an object multiplied by gravity, measured with a scale, using the gram as the basic unit.
  • Volume is the space occupied by a liquid or an object, with the liter as the basic unit; use graduated pipettes and graduated cylinders for measurement.
  • The meniscus is the curve at the surface of a liquid in a measuring instrument.
  • Read the bottom of the meniscus at eye level and use a meniscus card for accurate reading.
  • Temperature measures how hot or cold something is, using Celsius as the basic unit; a thermometer is used to measure temperature.
  • Diameter at breast height (DBH) is a standard measure of tree diameter.
  • DBH can be calculated by dividing the circumference of the tree by pi (3.14).

Scientific Investigation and Writing

  • Scientific investigation follows the process of the scientific method.
  • Observation involves observing an aspect, phenomenon, or problem.
  • Questions involve formulating inquiries to challenge the observation.
  • Literature Review involves seeing if the question has been asked before and conducting background research.
  • Hypothesis involves a potential answer to the question posed.
  • Prediction involves an expected result if the hypothesis is correct.
  • Experiment involves designing and conducting a test of the hypothesis.
  • Conclusion involves interpreting and discussing the results.
  • Limitations of the scientific method include human error.
  • Formulate testable hypotheses and predictions, such as "Pesticides are lethal to termites; termites will die when sprayed with pesticide."
  • Important factors in experimental design include experimental group, control group, sample size, and replicability.
  • Types of variables include independent, dependent, controlled, discrete, and continuous variables.
  • Independent variables are manipulated throughout the experiment.
  • Dependent variables are observed.
  • Controlled variables are kept the same across all groups.
  • Continuous variables are measured quantitatively and use a logical set of numbers.
  • Discrete variables are measured qualitatively and use categories.
  • The experimental group receives treatment with the independent variable, while replication ensures the experiment can be replicated, and sample size helps the accuracy of results.
  • Use line graphs when the independent variable is continuous, and bar graphs when it is discrete.
  • Histograms represent a range of independent variable values with bars.
  • Develop reasonable conclusions based on collected data to form a theory.
  • Sunscreen protects from UV radiation and affects exposure to it.

Parts of a Scientific Paper

  • The title provides a one-sentence description of the paper's content.
  • Authors include the scientists who contributed most to the research.
  • Keywords are assigned by the author to describe the paper and aid in database searches.
  • Abstract is a one-paragraph summary of the most important information, typically written last.
  • Introduction gives background information and poses the research question.
  • Materials and Methods describe exactly how experiments were performed.
  • Results summarize findings and present data in tables and figures.
  • Discussion interprets the results in the context of the research question.
  • Conclusion summarizes the most important findings of the work and their meaning.
  • Acknowledgements thank those who contributed to the research (funding or assistance).
  • References/Literature Cited list any sources used in the article.

Allelopathy: A Plant-Plant Interaction

  • Allelopathy is when plants release harmful chemicals that are detrimental to neighboring plants.
  • Plant competition occurs when plants need resources like sunlight and water, and other plants inhibit this; species interactions include predation, herbivory, mutualism, or parasitism.
  • Plant compounds inhibit seedling growth and reproduction.
  • Allelochemicals are compounds not directly involved in primary plant functions.
  • Testing essential oils aims to determine if they are a viable option for bioherbicides, which can be used to control an invasive plant that is allelopathic.

Explain Classes Of Secondary Compounds

  • Alkaloids are alkaline nitrogen-containing compounds with pharmacological effects, like coffee, tobacco, and chocolate.
  • Terpenes compose the largest group of plant chemicals, including essential oils like catnip and THC.
  • Phenols contain a hydroxyl group bonded to an aromatic benzene ring, such as vanilla extract.

Building Green

  • Building green is the practice of creating environmentally and people-friendly buildings, promoting native species, protecting from invasive species, and reducing greenhouse gases.
  • Sustainability refers to the ability to use resources in a way that meets present and future human requirements without harming the environment.
  • LEED certification is obtained by earning points for prerequisites and credits that address carbon, energy, water, waste, transportation, materials, health, and indoor environmental quality.
  • Energy use is calculated by multiplying average energy use by the hours of bulb use and dividing by 1000.
  • Annual energy use is found by dividing energy use by 1000 and multiplying it by hours per day and days per year.
  • Annual cost is found by multiplying kilowatt hours per year by cost per kilowatt.
  • Measure the energy consumption and kilowattage of each bulb to calculate lighting costs.
  • Minimize environmental impact with lights that automatically turn off, rain chains, and gutter shoots.
  • Environmentally sustainable practices help the planet and people.
  • Reduce environmental impact by recycling plastics and using sustainable transportation.

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