36 Questions
What is the primary purpose of wearing a lab coat in the laboratory?
To protect your skin from exposure to hazardous materials
What should you do in case of a spill or contamination in the laboratory?
Clean up the spill and report it to the laboratory authorities
Why is it important to follow the rules and organization of the laboratory?
To ensure a safe and efficient working environment
What should you do with used materials, such as PPEs, needles, and sharps?
Dispose of them in respective waste disposal systems
Why is it important to keep long hair packed in the laboratory?
To prevent hair from getting caught in equipment
What should you do in case of a fire in the laboratory?
All of the above
What is the primary goal of animal ethics?
To respect the intrinsic value of animals and their capacity to feel pain
Why is it important to maintain equipment in top form?
To keep it in place, calibrated, protected, and functional
What is the importance of effective communication when working with others in experimentation?
To facilitate the understanding of roles and responsibilities
What is the primary reason for respecting the intrinsic value of animals?
Because animals are sentient creatures
What is the main principle of facilitation in a laboratory setting?
To make things as easy as possible
What is the primary goal of keeping rules and instructions handy?
To ensure that users understand how to use the equipment safely
What is the importance of being happy to share knowledge and skills in a laboratory setting?
To ensure that all team members have access to necessary information
What is the primary goal of maintaining rosters, schedules, and critical arrangements?
To ensure that all team members are aware of their roles and responsibilities
What do our attitudes towards animals, including their use in research, reflect?
Our moral actions
What is one of the factors that enable comfort in the Environment domain of animal welfare?
Predictability and temperature control
What is the primary goal of the Health domain of animal welfare?
To ensure good health through the absence of disease and injury
What is the guiding principle of animal ethics committees when reviewing research protocols?
The Three Rs
What is the primary goal of the 'Replace' principle in the 3 Rs?
To use non-animal experimentation methods
What is a key requirement for researchers handling animals used in experimentation?
Specific knowledge about the biology of the animal species
What is the primary goal of the 'Refine' principle in the 3 Rs?
To avoid hurting and suffering the animal
What is the purpose of weighing benefit against harm in animal research?
To ensure that the benefits of the research outweigh the potential harm to the animals
What type of waste includes broken glass, needles, and razor blades?
Regulated medical waste
What should be done to bottles before disposing of them?
Remove the tops and ensure no detectable smell of chemicals
What is a pathogen?
A type of biological material that poses a risk for human health
What type of waste is disposed of in accordance with the Radiation Safety Manual?
Radioactive waste
What is the purpose of special waste containers?
To handle and dispose of special types of waste
What is one way that pathogens can be transmitted?
Through contact with an exposed part of the body
What is an important aspect of biosafety?
The use of microbiological techniques
Why is it important to follow proper disposal procedures for laboratory waste?
To ensure laboratory safety
What is the main goal of ethical laboratory waste management?
To protect the environment and human health
What is the purpose of biosafety levels?
To determine the level of risk associated with working with a particular pathogen
What is an example of a pathogen that is typically handled in a BSL-1 laboratory?
E. coli
What is a key aspect of biosafety training?
Teaching laboratory workers how to handle accidental ingestion
What is an important aspect of biocontainment?
The use of biosafety cabinets
What is the purpose of medical and laboratory waste management?
To prevent the spread of disease
Study Notes
Laboratory Conduct and Safety
- Properly cover up with protective dress to avoid exposing skin, and wear lab coats, gloves, goggles, and earplugs as necessary.
- Wear comfortable clothing and keep long hair packed.
- Keep bags, umbrellas, and other possessions away from laboratory benches.
Preparing for Eventualities
- Know and follow laboratory rules and organization.
- Be aware of potential hazards in the lab.
- Know the location of safety equipment and facilities, such as eye wash, shower, fire extinguisher, and fire blanket.
- Be prepared to lend a hand in case of emergencies.
Following Laboratory Rules
- Maintain a clean and functional laboratory by cleaning up spills, recalibrating equipment, and reporting breakages.
- Follow rules for labeling and handling chemicals and reagents.
- Use bio resources ethically and dispose of used materials in respective waste disposal systems.
- Follow safety procedures for using flames, radiation, noisy equipment, and volatile substances.
Avoiding Contamination
- Avoid contaminating surfaces, compartments, utensils, equipment, supplies, buffers, and liquids and solutions.
Sharing Common Supplies and Equipment
- Follow agreed procedures for sharing supplies and equipment.
- Label items well, clean and restore them after use, and return them to their place.
- Keep equipment in top form and maintain user-friendly conditions.
Working with Others
- Practice effective communication, punctuality, and timing.
- Understand and fulfill your role, and ensure your own competence and reliability.
Sharing Knowledge and Resources
- Share knowledge, know-how, skills, and techniques.
- Share facilities, rules, and instructions, and maintain scientific and professional news and announcements.
Ethical Principles
- Prioritize safety, cordiality, facilitation, efficiency, and productivity.
- Practice surveillance, avoid harm to self, others, property, and the environment.
Animal Ethics
- Animals have intrinsic value and should be treated with respect.
- Animals are sentient creatures with the capacity to feel pain, and their interests should be considered.
Five Domains of Animal Welfare
- Nutrition: ensure access to sufficient, balanced, varied, and clean food and water.
- Environment: provide comfort through temperature, substrate, space, air, odor, noise, and predictability.
- Health: maintain good health through the absence of disease, injury, and impairment.
- Behaviour: provide varied, novel, and engaging environmental challenges.
- Mental State: ensure positive states, such as pleasure, comfort, or vitality, and reduce negative states.
The Three Rs
- Replace: use non-animal experimentation when possible.
- Reduce: minimize the use of animals to obtain reliable data.
- Refine: avoid hurting and suffering the animal, and minimize pain, suffering, distress, and lasting harm.
The Researcher Using Animals
- Researchers should be trained in the biology of the animal species and know how to take care of the animals.
- Researchers should be aware of the risks of microbial infectious agents, biological materials, and biologically derived toxins.
Biosafety
- Biosafety involves effective policies, risk identification, assessment, and continuous monitoring, training, and cooperation.
- Biosafety levels (BSL-1, BSL-2, BSL-3, and BSL-4) ensure different levels of protection when working with virulent microbial strains.
- BSL-1 involves basic teaching or research laboratory work with non-pathogenic strains.
Laboratory Waste Management
- Dispose of biological waste, regulated medical waste, and radioactive waste according to appropriate manuals and instructions.
- Use special waste containers (bins, boxes) for biological waste, regulated medical waste, and radioactive waste.
- Remove tops from bottles before disposal and ensure no detectable smell of chemicals from any bottle placed in the bin.
This quiz covers essential guidelines for laboratory safety, including dress code, personal protective equipment, and being prepared for eventualities.
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