Laboratory Diagnosis of Opportunistic Aspergillosis and Candidiasis

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17 Questions

What is the recommended method for collecting blood specimens for fungal diagnosis?

Collect aseptically using a syringe and needle

For how long can nails specimens be transported at room temperature after collection?

< 72 hours

Which specimen collection method is recommended for eye corneal scrapings for fungal diagnosis?

Inoculated directly

What is the appropriate technique for collecting skin specimens for fungal diagnosis?

Scrape with a dull edge of scalpel or glass slide

Which body fluid collection method is NOT recommended for fungal diagnosis?

Refrigerate the sample

How should medical devices collected surgically be transported for fungal diagnosis?

< 15 minutes: room temperature; if longer, 44°C

What is the preferred carbon source for fungi?

Maltose

Which of the following temperature ranges do most fungi grow within?

0 to 35°C

In lichen associations, what is the name given to the fungi component?

Mycobiont

Which type of lichen is characterized by a leafy appearance?

Foliose

What is the term for the association between plant roots and fungi?

Mycorrhizae

Which of the following is NOT a use of mycorrhizae?

Determination of rock age

What is the purpose of using Dermatophyte Test Medium?

To diagnose dermatophyte infections by color change

Which agar promotes the mold to yeast conversion of several fungal species such as Histoplasma capsulatum and Blastomyces dermatitidis?

Blood Glucose Cysteine Agar

What is the purpose of using Niger Seed And Esculin Base Medium?

Selective isolation and identification of Cryptococcus spp

Which media stimulates the production of spores and sporulating bodies?

PDA And Potato Flake Agar

For what purpose is Yeast Extract Phosphate agar primarily used?

Presumptive identification of Candida spp

This quiz covers specimen collection, handling, and transport guidelines for opportunistic Aspergillosis caused by Aspergillus species and Candidiasis caused by Candida albicans. Topics include biosafety cabinet use, transportation in leak-proof sterile containers, and the importance of immediate processing. Part of Module 5 on Fungi as Human Pathogens.

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