Podcast
Questions and Answers
What is a recommended initial action to take once a disease is diagnosed in a lab animal?
What is a recommended initial action to take once a disease is diagnosed in a lab animal?
- Place the animal in quarantine (correct)
- Conduct no further actions
- Increase the animal's food intake
- Immediately release the animal into the wild
Which of the following is NOT considered a non-infectious cause of disease in lab animals?
Which of the following is NOT considered a non-infectious cause of disease in lab animals?
- Trauma
- Bacterial infection (correct)
- Housing conditions
- Toxins
What is one consequence of continuous tooth growth in rodents?
What is one consequence of continuous tooth growth in rodents?
- Enhanced chewing ability
- Increased vocalization
- Improved dental hygiene
- Incisor overgrowth (correct)
Which of the following treatments is specifically mentioned as sometimes being used in cases of disease in lab animals?
Which of the following treatments is specifically mentioned as sometimes being used in cases of disease in lab animals?
What behavior is reflected in the term 'barbering' in lab rodents?
What behavior is reflected in the term 'barbering' in lab rodents?
What is a common symptom of Mycoplasma infections in mice and rats?
What is a common symptom of Mycoplasma infections in mice and rats?
Which of the following is considered a general behavioral symptom of disease in animals?
Which of the following is considered a general behavioral symptom of disease in animals?
Which bodily position is indicative of illness in an animal?
Which bodily position is indicative of illness in an animal?
What condition is characterized by the inability to properly react or engage with the environment in animals?
What condition is characterized by the inability to properly react or engage with the environment in animals?
Which symptom can indicate dehydration in laboratory animals?
Which symptom can indicate dehydration in laboratory animals?
What are potential respiratory symptoms caused by severe Mycoplasma infections?
What are potential respiratory symptoms caused by severe Mycoplasma infections?
What is a potential general symptom related to feed and water uptake in sick animals?
What is a potential general symptom related to feed and water uptake in sick animals?
Which symptom is commonly associated with severe infections, such as those caused by Mycoplasma?
Which symptom is commonly associated with severe infections, such as those caused by Mycoplasma?
What type of implantation occurs in humans?
What type of implantation occurs in humans?
During amniogenesis, how does the amnion develop in mice?
During amniogenesis, how does the amnion develop in mice?
Which term describes the separation of the inner cell mass into the embryo and amnion in humans?
Which term describes the separation of the inner cell mass into the embryo and amnion in humans?
What type of implantation is characteristic of pigs?
What type of implantation is characteristic of pigs?
What crucial step occurs prior to the folding that leads to plectamnion in rodents?
What crucial step occurs prior to the folding that leads to plectamnion in rodents?
Which of the following correctly identifies the two major types of amniogenesis?
Which of the following correctly identifies the two major types of amniogenesis?
In mammals, what surrounds the embryo or fetus within the amniotic cavity?
In mammals, what surrounds the embryo or fetus within the amniotic cavity?
What is the fate of the ectoplacental cavity during amniogenesis in rodents?
What is the fate of the ectoplacental cavity during amniogenesis in rodents?
What defines the placental barrier in terms of its classification?
What defines the placental barrier in terms of its classification?
Which type of placenta is characterized by having six tissue layers?
Which type of placenta is characterized by having six tissue layers?
In which species is the epitheliochorial type of placental barrier found?
In which species is the epitheliochorial type of placental barrier found?
How many layers were identified in the human placenta, according to the Grosser classification?
How many layers were identified in the human placenta, according to the Grosser classification?
What unique characteristic is observed in the pig's placenta regarding its capillaries?
What unique characteristic is observed in the pig's placenta regarding its capillaries?
The term 'haemomonochorial' indicates what about the placenta?
The term 'haemomonochorial' indicates what about the placenta?
Which placental classification indicates a totally non-invasive placenta?
Which placental classification indicates a totally non-invasive placenta?
What phenomenon has been suggested to characterize the placenta in pigs?
What phenomenon has been suggested to characterize the placenta in pigs?
What is the primary site where villi are found in the pig placenta?
What is the primary site where villi are found in the pig placenta?
Which type of placenta does NOT have microvilli present?
Which type of placenta does NOT have microvilli present?
What characterizes the areolae in the pig placenta?
What characterizes the areolae in the pig placenta?
Which statement is FALSE regarding paraplacental structures?
Which statement is FALSE regarding paraplacental structures?
Which function is primarily associated with areolae in the pig placenta?
Which function is primarily associated with areolae in the pig placenta?
Which of the following is NOT a characteristic used to classify placentas?
Which of the following is NOT a characteristic used to classify placentas?
What is a primary function of paraplacental structures?
What is a primary function of paraplacental structures?
Which characteristic about fetal cells is correct regarding the endothelial interface?
Which characteristic about fetal cells is correct regarding the endothelial interface?
Which of the following species belongs to the family Leporidae?
Which of the following species belongs to the family Leporidae?
What is the correct sequence in the taxonomic hierarchy for Canis lupus?
What is the correct sequence in the taxonomic hierarchy for Canis lupus?
Which of the following is NOT classified under the class Mammalia?
Which of the following is NOT classified under the class Mammalia?
Which family does the Rhesus monkey belong to?
Which family does the Rhesus monkey belong to?
What is the species epithet of Ovis aries?
What is the species epithet of Ovis aries?
Which of these genera is associated with the family Muridae?
Which of these genera is associated with the family Muridae?
Identify the correct binomen for the guinea pig.
Identify the correct binomen for the guinea pig.
Which order does Ovis aries belong to?
Which order does Ovis aries belong to?
Which class includes both fish and mammals?
Which class includes both fish and mammals?
Which of the following species is classified under the family Caviidae?
Which of the following species is classified under the family Caviidae?
How can you prevent wear on your needle holder?
How can you prevent wear on your needle holder?
Which of the following is a sedative?
Which of the following is a sedative?
What is correct?
What is correct?
What epitopes are necessary to produce antibodies?
What epitopes are necessary to produce antibodies?
Why do we use a linear model to estimate the exposure risk at low doses?
Why do we use a linear model to estimate the exposure risk at low doses?
What is the correct statement for bipolar electrosurgery?
What is the correct statement for bipolar electrosurgery?
Which of the following has the clearest sedative capacities?
Which of the following has the clearest sedative capacities?
What should never be added simultaneously with tetracyclines?
What should never be added simultaneously with tetracyclines?
To induce a specific IgG response, an antigen needs:
To induce a specific IgG response, an antigen needs:
What is allodynia?
What is allodynia?
How do you maintain the lifespan of a needle holder?
How do you maintain the lifespan of a needle holder?
Which of the following statements concerning pheromones in mouse reproduction is true?
Which of the following statements concerning pheromones in mouse reproduction is true?
What is the Bruce effect?
What is the Bruce effect?
Which type of euthanasia is not allowed for a conscious mouse?
Which type of euthanasia is not allowed for a conscious mouse?
What is not allowed in Belgium?
What is not allowed in Belgium?
How do you best protect the needle holdeR?
How do you best protect the needle holdeR?
What is the best sedative?
What is the best sedative?
What is the best excretion method for removing a drug from the body besides glomerular filtration?
What is the best excretion method for removing a drug from the body besides glomerular filtration?
Which statement is correct?
Which statement is correct?
Extrapolation of low radiation doses: Which statement is correct?
Extrapolation of low radiation doses: Which statement is correct?
Explain the 3Rs and give examples.
Explain the 3Rs and give examples.
Describe the four basic principles of anesthesia and provide an example of medication for each.
Describe the four basic principles of anesthesia and provide an example of medication for each.
Why is it important for small laboratory animals to awaken quickly from anesthesia?
Why is it important for small laboratory animals to awaken quickly from anesthesia?
Explain: incubation period, facultative pathogen, and subclinical carrier.
Explain: incubation period, facultative pathogen, and subclinical carrier.
Which of the following is the most sedative?
Which of the following is the most sedative?
Which imaging techniques are functional?
Which imaging techniques are functional?
What is the advantage of MRI and CT?
What is the advantage of MRI and CT?
What is the main advantage of MRI and CT, in comparison to conventional radiography?
What is the main advantage of MRI and CT, in comparison to conventional radiography?
Why does a linear model instead of a quadratic model fit the last slide of the lecture better?
Why does a linear model instead of a quadratic model fit the last slide of the lecture better?
Compare the dentition of rabbits and rats: provide the dental formula, total number of teeth, and which grow continuously.
Compare the dentition of rabbits and rats: provide the dental formula, total number of teeth, and which grow continuously.
What is the difference between physiological and pathological pain?
What is the difference between physiological and pathological pain?
What are the different phases of anesthesia?
What are the different phases of anesthesia?
Flashcards
Interstitial Implantation
Interstitial Implantation
Implantation where the embryo burrows into the uterine wall, eventually losing contact with the uterine lumen.
Superficial Implantation
Superficial Implantation
Implantation where the embryo remains within the uterine lumen throughout pregnancy, never burrowing into the uterine wall
Amniogenesis
Amniogenesis
The process by which the amnion, a membrane filled with amniotic fluid, forms around the embryo.
Plectamnion
Plectamnion
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Schizamnion
Schizamnion
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Proamniotic Cavity Formation
Proamniotic Cavity Formation
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Ectoplacental Cavity
Ectoplacental Cavity
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Rodent Amniogenesis
Rodent Amniogenesis
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Incisor Overgrowth
Incisor Overgrowth
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Barbering
Barbering
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Environmental Factors
Environmental Factors
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Trauma
Trauma
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Innate or Hereditary Factors
Innate or Hereditary Factors
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Labyrinthine placenta
Labyrinthine placenta
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Placental barrier
Placental barrier
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Epitheliochorial placenta
Epitheliochorial placenta
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Grosser classification
Grosser classification
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Placental invasion
Placental invasion
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Trophoblast
Trophoblast
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Syncytiotrophoblast
Syncytiotrophoblast
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Haemomonochorial placenta
Haemomonochorial placenta
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Scurvy (Scheurbuik)
Scurvy (Scheurbuik)
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Mycoplasma Infection
Mycoplasma Infection
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Torticollis
Torticollis
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Pneumonia
Pneumonia
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Behavioral Changes in Animals
Behavioral Changes in Animals
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Abnormal Posture in Animals
Abnormal Posture in Animals
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Changes in Feeding and Water Uptake
Changes in Feeding and Water Uptake
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Loss of Appetite
Loss of Appetite
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Placenta Classification Criteria
Placenta Classification Criteria
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Paraplacental Structures
Paraplacental Structures
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Areolae in Pigs
Areolae in Pigs
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Endotheliochorial Placenta
Endotheliochorial Placenta
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Microvilli in Placentas
Microvilli in Placentas
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Histiotrophic Nutrition
Histiotrophic Nutrition
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Erythrocytophagocytosis
Erythrocytophagocytosis
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Hemotrophic Nutrition
Hemotrophic Nutrition
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Binomen
Binomen
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Genus
Genus
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Family
Family
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Order
Order
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Class
Class
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Phylum
Phylum
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Species Epithet
Species Epithet
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Vertebrates
Vertebrates
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Mammals
Mammals
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Taxonomic Hierarchy
Taxonomic Hierarchy
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Study Notes
Course Information
- Course: Advanced Plant Cell Biology and Signaling | Master Biomedical and Plant Biotechnology
- Academic Year: 2020-2021
- Answers LAS Exam Questions
Content
- Introduction to laboratory animal science (Prof. dr. K. Hermans)
- Postmortem examination (Prof. dr. K. Hermans)
- Embryology (Prof. dr. P. Cornillie)
- Diseases and disease control (Prof. dr. K. Hermans)
- Taxonomy (Prof. dr. P. Cornillie)
- Reproduction and reproductive techniques (Prof. dr. A. Van Soom)
- Comparative morphology (Prof. dr. W. De Spiegelaere)
- Principles of surgery in experimental animals (Prof. dr. A. Martens)
- Nutrition of laboratory animals (Prof. dr. G. Janssens)
- Basic principles of pharmacokinetics and pharmacodynamics (Prof. dr. M. Devreese)
- Comparative physiology (Drs. C. De Meeûs)
- Immunology (Prof. dr. E. Cox)
- Postoperative care (Prof. dr. K. Hermans)
- Experimental techniques (Prof. dr. K. Hermans)
- Chapter 15: Medical Imaging
- Chapter 16: Radiobiology and radioprotection
- Chapter 17: Analgesia and anaesthesia
- Chapter 18: Laboratory animal legislation
- Chapter 19: Ethical committees
- Chapter 20 & 21: Ethology & stress: Animal welfare, Housing.
- Chapter 22: Environmental enrichment
- Chapter 23: Statistics
- Topic 24: Extrapolation of experimental data to man
- Topic 25: The ethics of animal use
- Topic 26: Safety in working with laboratory animals
- Topic 27: Viral zoonoses
- Topic 28: Bacterial zoonoses
- Topic 29: Euthanasia of laboratory animals
- Topic 30: Transportation of laboratory animals
- Disease prevention & hygiene
- GLP
- Genetics
Exam Preparation LAS H1-14
- 3Rs: Replacement, Reduction, Refinement
- Replacement: methods avoiding animal use (e.g. in vitro models)
- Reduction: methods for reducing the number of animals or getting more information from fewer animals
- Refinement: methods improving animal welfare
- Non-technical summary (NTS): meant for the general public, explaining benefits of the project, how many animals are needed, and how they're treated
- Types of animals: Category A, B, C, D (caretakers, experimenters, directors, specialists)
Introduction in Laboratory Animal Science
- Three Rs
- Replacement (e.g., in vitro models, computerized models)
- Reduction (e.g., animal sharing, improved statistical design)
- Refinement (e.g., better techniques/equipment)
- NTS (Non-Technical Summary)
- Explanation of 3R aspects
Postmortem Examination
- Sample conservation and shipment
- Formalin-fixed tissue preservation
- Use 10% neutral buffered formalin
- Use 10x more fixative than tissue
- Steps in Postmortem examination
- External & macroscopic evaluation of organs
- Microscopy of gut content
- Cytology
- Histology
- Bacteriology
- Virology
Embryology
- Species-specific differences (placentation, organogenesis)
- Critical period of organogenesis = Most vulnerable time for malformations
- Teratogens = factors causing malformations
- Limb development critical periods: Man(wk 4-7), Cat(wk 4-6), Dog(wk 3-4), Rabbit/Rat(wk 2-3)
Diseases and Disease Control
- Impact of disease on experimental results (subclinical carriers)
- Incubation period
- Facultative pathogens
- Diseases of Pasteurella multocida in rabbits (e.g., exophthalmia, conjunctivitis, rhinitis)
- Importance on animal welfare, transmission of diseases from animals to humans (zoonotic)
- Epizootic
- Enzootic
- Quarantine
- Subclinical Carriers
Comparative Morphology
- 5 major anatomical differences between rabbits and rodents (e.g., teeth, stomach, respiratory system, limbs, etc.)
- Explanation of vertebral formula
- Differences in body surface area across species
Reproduction and Reproductive Techniques
- Difference between copulatory plug/post-ovulatory discharge (different species, e.g., golden hamster)
- Breeding schemes pros/cons
- Effects of pheromones
- Bruce effect
Additional Topics (Chapters 15-30)
- These chapters cover additional topics related to laboratory animal science, including Specific and/or general examples
- Specific examples refer to the type of animal being used, and general examples applies to all
- Explanations, descriptions, or types of methods
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