Lab Animal Health and Disease Assessment

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Questions and Answers

What is a recommended initial action to take once a disease is diagnosed in a lab animal?

  • Place the animal in quarantine (correct)
  • Conduct no further actions
  • Increase the animal's food intake
  • Immediately release the animal into the wild

Which of the following is NOT considered a non-infectious cause of disease in lab animals?

  • Trauma
  • Bacterial infection (correct)
  • Housing conditions
  • Toxins

What is one consequence of continuous tooth growth in rodents?

  • Enhanced chewing ability
  • Increased vocalization
  • Improved dental hygiene
  • Incisor overgrowth (correct)

Which of the following treatments is specifically mentioned as sometimes being used in cases of disease in lab animals?

<p>Vaccination (A)</p> Signup and view all the answers

What behavior is reflected in the term 'barbering' in lab rodents?

<p>Abnormal grooming behavior (A)</p> Signup and view all the answers

What is a common symptom of Mycoplasma infections in mice and rats?

<p>Torticollis (D)</p> Signup and view all the answers

Which of the following is considered a general behavioral symptom of disease in animals?

<p>Loss of grooming (A)</p> Signup and view all the answers

Which bodily position is indicative of illness in an animal?

<p>Hunched back (D)</p> Signup and view all the answers

What condition is characterized by the inability to properly react or engage with the environment in animals?

<p>Slow or lack of reaction (A)</p> Signup and view all the answers

Which symptom can indicate dehydration in laboratory animals?

<p>Loss of appetite (A)</p> Signup and view all the answers

What are potential respiratory symptoms caused by severe Mycoplasma infections?

<p>Pneumonia and rhinitis (B)</p> Signup and view all the answers

What is a potential general symptom related to feed and water uptake in sick animals?

<p>Weight loss (B)</p> Signup and view all the answers

Which symptom is commonly associated with severe infections, such as those caused by Mycoplasma?

<p>Oedematous swelling on ears and eyes (D)</p> Signup and view all the answers

What type of implantation occurs in humans?

<p>Interstitial implantation (D)</p> Signup and view all the answers

During amniogenesis, how does the amnion develop in mice?

<p>By cavitation and plectamnion (A)</p> Signup and view all the answers

Which term describes the separation of the inner cell mass into the embryo and amnion in humans?

<p>Schizamnion (B)</p> Signup and view all the answers

What type of implantation is characteristic of pigs?

<p>Superficial implantation (A), Central implantation (C)</p> Signup and view all the answers

What crucial step occurs prior to the folding that leads to plectamnion in rodents?

<p>Hollowing of the pro-amniotic cavity (B)</p> Signup and view all the answers

Which of the following correctly identifies the two major types of amniogenesis?

<p>Schizamnion and plectamnion (C)</p> Signup and view all the answers

In mammals, what surrounds the embryo or fetus within the amniotic cavity?

<p>Amnion (A)</p> Signup and view all the answers

What is the fate of the ectoplacental cavity during amniogenesis in rodents?

<p>It merges with the amniotic cavity (A)</p> Signup and view all the answers

What defines the placental barrier in terms of its classification?

<p>The number of tissue layers nutrients must cross (D)</p> Signup and view all the answers

Which type of placenta is characterized by having six tissue layers?

<p>Epitheliochorial (C)</p> Signup and view all the answers

In which species is the epitheliochorial type of placental barrier found?

<p>Horse (D)</p> Signup and view all the answers

How many layers were identified in the human placenta, according to the Grosser classification?

<p>Two layers (C)</p> Signup and view all the answers

What unique characteristic is observed in the pig's placenta regarding its capillaries?

<p>They nestle between the epithelial cells (C)</p> Signup and view all the answers

The term 'haemomonochorial' indicates what about the placenta?

<p>One layer is separated (A)</p> Signup and view all the answers

Which placental classification indicates a totally non-invasive placenta?

<p>Epitheliochorial (A)</p> Signup and view all the answers

What phenomenon has been suggested to characterize the placenta in pigs?

<p>Potential endothelioendothelial type (C)</p> Signup and view all the answers

What is the primary site where villi are found in the pig placenta?

<p>At the site of the areolae (C)</p> Signup and view all the answers

Which type of placenta does NOT have microvilli present?

<p>Endotheliochorial placenta (B)</p> Signup and view all the answers

What characterizes the areolae in the pig placenta?

<p>Increased glandular activity (D)</p> Signup and view all the answers

Which statement is FALSE regarding paraplacental structures?

<p>They contribute to hemotrophic nutrition. (C)</p> Signup and view all the answers

Which function is primarily associated with areolae in the pig placenta?

<p>Transfer of iron (C)</p> Signup and view all the answers

Which of the following is NOT a characteristic used to classify placentas?

<p>Fetal gut development (C)</p> Signup and view all the answers

What is a primary function of paraplacental structures?

<p>Histiotrophic nutrition (A)</p> Signup and view all the answers

Which characteristic about fetal cells is correct regarding the endothelial interface?

<p>Fetal cells do not form microvilli. (B)</p> Signup and view all the answers

Which of the following species belongs to the family Leporidae?

<p>Oryctolagus cuniculus (B)</p> Signup and view all the answers

What is the correct sequence in the taxonomic hierarchy for Canis lupus?

<p>Phylum: Mammalia, Order: Carnivora, Family: Canidae, Genus: Canis, Species: lupus (A)</p> Signup and view all the answers

Which of the following is NOT classified under the class Mammalia?

<p>Zebrafish (C)</p> Signup and view all the answers

Which family does the Rhesus monkey belong to?

<p>Cercopithecidae (C)</p> Signup and view all the answers

What is the species epithet of Ovis aries?

<p>aries (D)</p> Signup and view all the answers

Which of these genera is associated with the family Muridae?

<p>Mus (B)</p> Signup and view all the answers

Identify the correct binomen for the guinea pig.

<p>Cavia procellus (B)</p> Signup and view all the answers

Which order does Ovis aries belong to?

<p>Ruminantia (C)</p> Signup and view all the answers

Which class includes both fish and mammals?

<p>Chordata (D)</p> Signup and view all the answers

Which of the following species is classified under the family Caviidae?

<p>Cavia procellus (A)</p> Signup and view all the answers

How can you prevent wear on your needle holder?

<p>Hold the needle closer to the tip (B)</p> Signup and view all the answers

Which of the following is a sedative?

<p>Atropine (B)</p> Signup and view all the answers

What is correct?

<p>A female mouse will abort if exposed to male urine within 24 hours (Bruce effect). (A), Lee-Boot effect is the cessation of the female cycle in the absence of males. (B)</p> Signup and view all the answers

What epitopes are necessary to produce antibodies?

<p>B and T epitopes (B)</p> Signup and view all the answers

Why do we use a linear model to estimate the exposure risk at low doses?

<p>To avoid underestimation. (B)</p> Signup and view all the answers

What is the correct statement for bipolar electrosurgery?

<p>Bipolar electrosurgery can only be used for coagulation, not incisions. (C)</p> Signup and view all the answers

Which of the following has the clearest sedative capacities?

<p>Acepromazine (ACP) (C)</p> Signup and view all the answers

What should never be added simultaneously with tetracyclines?

<p>Warfarin (C)</p> Signup and view all the answers

To induce a specific IgG response, an antigen needs:

<p>B and T cell epitopes (C)</p> Signup and view all the answers

What is allodynia?

<p>Sensation of pain coming from a stimulus that should not be painful. (B)</p> Signup and view all the answers

How do you maintain the lifespan of a needle holder?

<p>Use a needle holder with a longitudinal groove. (C)</p> Signup and view all the answers

Which of the following statements concerning pheromones in mouse reproduction is true?

<p>A male mouse will urinate on a female mouse before mating. (A)</p> Signup and view all the answers

What is the Bruce effect?

<p>A female mouse has an abortion within the first days of her pregnancy if she comes in close contact with male urine. (A)</p> Signup and view all the answers

Which type of euthanasia is not allowed for a conscious mouse?

<p>CO2 inhalation. (C)</p> Signup and view all the answers

What is not allowed in Belgium?

<p>Experiments on animals for tobacco research. (C)</p> Signup and view all the answers

How do you best protect the needle holdeR?

<p>Use a needle holder with a thick tip for thick needles. (B)</p> Signup and view all the answers

What is the best sedative?

<p>ACP (A)</p> Signup and view all the answers

What is the best excretion method for removing a drug from the body besides glomerular filtration?

<p>Tubular secretion. (A)</p> Signup and view all the answers

Which statement is correct?

<p>The premolars and molars of a rabbit grow constantly. (A)</p> Signup and view all the answers

Extrapolation of low radiation doses: Which statement is correct?

<p>A linear approach is used to avoid underestimating the risk. (A)</p> Signup and view all the answers

Explain the 3Rs and give examples.

<p>The 3Rs stand for Replacement, Reduction, and Refinement.</p> <ul> <li>Replacement means using alternatives to animals, such as in vitro methods, computer modeling, or human tissue cultures, whenever possible.</li> <li>Reduction involves using fewer animals or obtaining more information from the same number of animals. This could involve refining experimental design or using more sensitive and precise measurement techniques.</li> <li>Refinement aims to minimize pain and distress for animals. This can involve using better anesthetic techniques, providing more enriching environments, or ensuring proper husbandry practices.</li> </ul> Signup and view all the answers

Describe the four basic principles of anesthesia and provide an example of medication for each.

<p>The four basic principles of anesthesia are: Induction, Maintenance, Analgesia, and Reversal.</p> <ul> <li>Induction is the process of initiating anesthesia, usually by administering a drug intravenously or inhaling a gas. Examples of induction agents include propofol and isoflurane.</li> <li>Maintenance involves keeping the animal at a constant level of anesthesia throughout the procedure. This can be achieved by continuous administration of a drug. An example is isoflurane for inhalation anesthesia.</li> <li>Analgesia aims to relieve pain, which is often a component of anesthesia. Opioids such as morphine or fentanyl could be given for analgesia.</li> <li>Reversal is the process of reversing anesthesia, allowing the animal to wake up quickly and safely. Reversal agents are given to counteract the effects of the induction agent, such as Atipamezole for the reversal of dexmedetomidine.</li> </ul> Signup and view all the answers

Why is it important for small laboratory animals to awaken quickly from anesthesia?

<p>It is important for small laboratory animals to awaken quickly from anesthesia because prolonged anesthesia can lead to several complications, including hypothermia, respiratory issues, and muscle weakness. Quick recovery minimizes the risk of these problems and allows the animal to resume normal activity sooner, ensuring their well-being and the success of the experiment.</p> Signup and view all the answers

Explain: incubation period, facultative pathogen, and subclinical carrier.

<p>Incubation period is the time between exposure to a pathogen and the onset of clinical symptoms. A facultative pathogen is a microorganism that typically does not cause disease, but can cause illness in a weakened host or under certain conditions. A subclinical carrier is an individual who harbors a pathogen without exhibiting symptoms but can still transmit the infection to others.</p> Signup and view all the answers

Which of the following is the most sedative?

<p>Acepromazine (D)</p> Signup and view all the answers

Which imaging techniques are functional?

<p>Scintigraphy (C)</p> Signup and view all the answers

What is the advantage of MRI and CT?

<p>MRI provides detailed information about soft tissue structures, while CT is more effective for bone imaging. (C)</p> Signup and view all the answers

What is the main advantage of MRI and CT, in comparison to conventional radiography?

<p>MRI and CT provide more detailed images, as opposed to a flat 2D image. (B)</p> Signup and view all the answers

Why does a linear model instead of a quadratic model fit the last slide of the lecture better?

<p>The linear model is more conservative, and it is used to avoid underestimating the potential risk. (B)</p> Signup and view all the answers

Compare the dentition of rabbits and rats: provide the dental formula, total number of teeth, and which grow continuously.

<p>Rabbits have a dental formula of 2I 1/1, 3P 2/1 3M 3/3 and a total of 28 teeth. Their incisors and molars grow continuously, while premolars are diphyodont, meaning they are replaced once. Rats have a dental formula of 1I 1/1, 0P 0/0 3M 3/3 and a total of 16 teeth. Their incisors grow continuously. Molars are brachyodont, meaning they stop growing once they reach their full size.</p> Signup and view all the answers

What is the difference between physiological and pathological pain?

<p>Physiological pain is felt in proportion to the intensity of the stimulus, while pathological pain is often disproportionate to the stimulus. (B)</p> Signup and view all the answers

What are the different phases of anesthesia?

<p>The phases of anesthesia are: premedication, induction, maintenance, and recovery.</p> <ul> <li>Premedication involves administering drugs to prepare the animal for anesthesia, reduce stress, and minimize side effects.</li> <li>Induction refers to the process of initiating anesthesia, usually by administering a drug intravenously or inhaling a gas.</li> <li>Maintenance involves keeping the animal at a constant level of anesthesia throughout the procedure. This can be achieved by continuous administration of a drug.</li> <li>Recovery is the process of the animal regaining wakefulness after the effects of anesthesia have worn off.</li> </ul> Signup and view all the answers

Flashcards

Interstitial Implantation

Implantation where the embryo burrows into the uterine wall, eventually losing contact with the uterine lumen.

Superficial Implantation

Implantation where the embryo remains within the uterine lumen throughout pregnancy, never burrowing into the uterine wall

Amniogenesis

The process by which the amnion, a membrane filled with amniotic fluid, forms around the embryo.

Plectamnion

A type of amniogenesis where the amnion forms by folding in of the trophoblast tissue around the developing embryo.

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Schizamnion

A type of amniogenesis where the amnion forms by splitting of the inner cell mass. The inner cell mass separates into the embryo and the amniotic sac.

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Proamniotic Cavity Formation

The process by which the inner cell mass hollows to form a cavity that will give rise to, among other things, the amnion.

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Ectoplacental Cavity

The cavity above the embryo formed by the folding of the trophoblast in plectamnion amniogenesis.

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Rodent Amniogenesis

A type of amniogenesis seen in most rodents, except the hamster and guinea pig, where the amnion forms from the folding of the trophoblast.

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Incisor Overgrowth

This refers to the growth of rodent incisors, which can lead to problems if not properly managed. Rodents need to wear down their teeth by gnawing on appropriate materials.

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Barbering

This can be a cause of disease in rodents. When rodents don't have enough to chew on, they may start chewing the fur of other rodents, leading to skin damage and potential infections. This is often caused by boredom.

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Environmental Factors

This is a common non-infectious cause of disease in lab animals. It can include issues like poor diet, lack of space in their cage, and exposure to harmful substances.

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Trauma

This is a non-infectious cause of disease in lab animals. It can range from minor injuries to major trauma, leading to various problems.

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Innate or Hereditary Factors

This is a non-infectious cause of disease in lab animals, and it can be caused by genetics or the way the animal is living.

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Labyrinthine placenta

A complex network formed when the tips of neighboring villi fuse in the villous placenta. This type of placenta is found in carnivores, rodents, apes, and rabbits.

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Placental barrier

The number of tissue layers that nutrients must cross from the maternal blood to the fetal blood in the placenta. This classification helps understand how closely the maternal and fetal circulations are connected.

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Epitheliochorial placenta

A type of placental barrier where six layers of tissue separate the maternal and fetal circulations. This is the least invasive type.

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Grosser classification

The classification of placentas based on the number of tissue layers separating the maternal and fetal blood.

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Placental invasion

The process by which the placenta invades the uterine wall, allowing better nutrient exchange but potentially leading to complications.

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Trophoblast

The outermost layer of the placenta, formed from the fetal tissue. It directly interacts with the maternal blood.

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Syncytiotrophoblast

The layer of the trophoblast that directly contacts the maternal blood. It facilitates gas and nutrient exchange.

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Haemomonochorial placenta

A placenta type where the maternal capillaries are close to the fetal capillaries, reducing the number of tissue layers. It occurs in humans.

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Scurvy (Scheurbuik)

A condition characterized by symptoms like lethargy, bloody gums, internal bleeding, muscle weakness, bleeding in muscles, anemia, and bleeding in the paws.

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Mycoplasma Infection

A bacterial infection that can affect the respiratory system of mice and rats. Symptoms include torticollis and pneumonia.

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Torticollis

A condition where the head is tilted to one side. It can be caused by a variety of issues including ear infections, neurological problems, and infections like Mycoplasma.

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Pneumonia

Inflammation of the lungs, often caused by bacterial or viral infections. In rats, Mycoplasma can lead to a severe form.

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Behavioral Changes in Animals

A general symptom indicative of illness in animals. It refers to a change in behavior, including sluggishness, lack of social interaction, and reduced grooming.

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Abnormal Posture in Animals

A general symptom indicative of illness in animals. It refers to the posture or position of the animal, including hunched back, torticollis, abnormal limb position, and ruffled fur.

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Changes in Feeding and Water Uptake

A general symptom indicative of illness in animals. It refers to a change in feeding and water intake, including loss of appetite, weight loss, and dehydration.

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Loss of Appetite

Reduction in food consumption due to illness. It can be a key indicator of disease.

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Placenta Classification Criteria

Classification of placentas based on the shape of the structure, surface morphology of the chorion, placental barrier and endometrial invasion, trophoblast cytology, fate of the maternal endometrium, composing fetal membranes & vasculature, paraplacental structures, and implantation types.

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Paraplacental Structures

These are structures located outside of the main placental area, but still play a role in pregnancy. Examples include areolae in pigs, marginal hematomes, endometrial cups, and subplacenta.

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Areolae in Pigs

These are domes of trophoblast located on the endometrium in pigs. They have increased glandular activity and are sites of histiotrophic nutrition, playing a role in iron transfer. Numerous, branched chorionic villi float in the space between the chorion and endometrium.

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Endotheliochorial Placenta

The type of placental barrier present in carnivores where fetal cells form a layer around maternal vessels, but lack microvilli due to the absence of surface extensions on individual cells.

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Microvilli in Placentas

These are microscopic extensions of individual cells present in most placenta types, but absent in endotheliochorial placentas. They aid in nutrient and waste exchange.

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Histiotrophic Nutrition

This type of nutrition in placentas involves the uptake of nutrients directly from the maternal tissues, rather than through blood circulation. It plays a role in iron transfer in pigs with areolae.

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Erythrocytophagocytosis

The process of red blood cell breakdown and absorption. It's not a direct function of paraplacental structures, but may be involved in some contexts.

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Hemotrophic Nutrition

A type of placental nutrition in which nutrients are absorbed from the maternal blood circulation. It is the primary mode of nourishment in placentas and is not a function of paraplacental structures.

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Binomen

The scientific name of an organism, consisting of two parts: the genus name and the species epithet.

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Genus

A group of closely related species.

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Family

A group of closely related genera.

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Order

A group of closely related families.

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Class

A group of closely related orders.

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Phylum

A group of closely related classes.

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Species Epithet

The second part of a scientific name, specific to the particular species.

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Vertebrates

Animals with a vertebral column.

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Mammals

Animals that give birth to live young, usually after a period of gestation in the mother's womb.

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Taxonomic Hierarchy

A classification system used to organize living organisms into hierarchical groups based on their shared characteristics.

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Study Notes

Course Information

  • Course: Advanced Plant Cell Biology and Signaling | Master Biomedical and Plant Biotechnology
  • Academic Year: 2020-2021
  • Answers LAS Exam Questions

Content

  • Introduction to laboratory animal science (Prof. dr. K. Hermans)
  • Postmortem examination (Prof. dr. K. Hermans)
  • Embryology (Prof. dr. P. Cornillie)
  • Diseases and disease control (Prof. dr. K. Hermans)
  • Taxonomy (Prof. dr. P. Cornillie)
  • Reproduction and reproductive techniques (Prof. dr. A. Van Soom)
  • Comparative morphology (Prof. dr. W. De Spiegelaere)
  • Principles of surgery in experimental animals (Prof. dr. A. Martens)
  • Nutrition of laboratory animals (Prof. dr. G. Janssens)
  • Basic principles of pharmacokinetics and pharmacodynamics (Prof. dr. M. Devreese)
  • Comparative physiology (Drs. C. De Meeûs)
  • Immunology (Prof. dr. E. Cox)
  • Postoperative care (Prof. dr. K. Hermans)
  • Experimental techniques (Prof. dr. K. Hermans)
  • Chapter 15: Medical Imaging
  • Chapter 16: Radiobiology and radioprotection
  • Chapter 17: Analgesia and anaesthesia
  • Chapter 18: Laboratory animal legislation
  • Chapter 19: Ethical committees
  • Chapter 20 & 21: Ethology & stress: Animal welfare, Housing.
  • Chapter 22: Environmental enrichment
  • Chapter 23: Statistics
  • Topic 24: Extrapolation of experimental data to man
  • Topic 25: The ethics of animal use
  • Topic 26: Safety in working with laboratory animals
  • Topic 27: Viral zoonoses
  • Topic 28: Bacterial zoonoses
  • Topic 29: Euthanasia of laboratory animals
  • Topic 30: Transportation of laboratory animals
  • Disease prevention & hygiene
  • GLP
  • Genetics

Exam Preparation LAS H1-14

  • 3Rs: Replacement, Reduction, Refinement
  • Replacement: methods avoiding animal use (e.g. in vitro models)
  • Reduction: methods for reducing the number of animals or getting more information from fewer animals
  • Refinement: methods improving animal welfare
  • Non-technical summary (NTS): meant for the general public, explaining benefits of the project, how many animals are needed, and how they're treated
  • Types of animals: Category A, B, C, D (caretakers, experimenters, directors, specialists)

Introduction in Laboratory Animal Science

  • Three Rs
  • Replacement (e.g., in vitro models, computerized models)
  • Reduction (e.g., animal sharing, improved statistical design)
  • Refinement (e.g., better techniques/equipment)
  • NTS (Non-Technical Summary)
  • Explanation of 3R aspects

Postmortem Examination

  • Sample conservation and shipment
  • Formalin-fixed tissue preservation
  • Use 10% neutral buffered formalin
  • Use 10x more fixative than tissue
  • Steps in Postmortem examination
  • External & macroscopic evaluation of organs
  • Microscopy of gut content
  • Cytology
  • Histology
  • Bacteriology
  • Virology

Embryology

  • Species-specific differences (placentation, organogenesis)
  • Critical period of organogenesis = Most vulnerable time for malformations
  • Teratogens = factors causing malformations
  • Limb development critical periods: Man(wk 4-7), Cat(wk 4-6), Dog(wk 3-4), Rabbit/Rat(wk 2-3)

Diseases and Disease Control

  • Impact of disease on experimental results (subclinical carriers)
  • Incubation period
  • Facultative pathogens
  • Diseases of Pasteurella multocida in rabbits (e.g., exophthalmia, conjunctivitis, rhinitis)
  • Importance on animal welfare, transmission of diseases from animals to humans (zoonotic)
  • Epizootic
  • Enzootic
  • Quarantine
  • Subclinical Carriers

Comparative Morphology

  • 5 major anatomical differences between rabbits and rodents (e.g., teeth, stomach, respiratory system, limbs, etc.)
  • Explanation of vertebral formula
  • Differences in body surface area across species

Reproduction and Reproductive Techniques

  • Difference between copulatory plug/post-ovulatory discharge (different species, e.g., golden hamster)
  • Breeding schemes pros/cons
  • Effects of pheromones
  • Bruce effect

Additional Topics (Chapters 15-30)

  • These chapters cover additional topics related to laboratory animal science, including Specific and/or general examples
  • Specific examples refer to the type of animal being used, and general examples applies to all
  • Explanations, descriptions, or types of methods

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