Lab Animal Health and Disease Assessment
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Questions and Answers

What is a recommended initial action to take once a disease is diagnosed in a lab animal?

  • Place the animal in quarantine (correct)
  • Conduct no further actions
  • Increase the animal's food intake
  • Immediately release the animal into the wild
  • Which of the following is NOT considered a non-infectious cause of disease in lab animals?

  • Trauma
  • Bacterial infection (correct)
  • Housing conditions
  • Toxins
  • What is one consequence of continuous tooth growth in rodents?

  • Enhanced chewing ability
  • Increased vocalization
  • Improved dental hygiene
  • Incisor overgrowth (correct)
  • Which of the following treatments is specifically mentioned as sometimes being used in cases of disease in lab animals?

    <p>Vaccination</p> Signup and view all the answers

    What behavior is reflected in the term 'barbering' in lab rodents?

    <p>Abnormal grooming behavior</p> Signup and view all the answers

    What is a common symptom of Mycoplasma infections in mice and rats?

    <p>Torticollis</p> Signup and view all the answers

    Which of the following is considered a general behavioral symptom of disease in animals?

    <p>Loss of grooming</p> Signup and view all the answers

    Which bodily position is indicative of illness in an animal?

    <p>Hunched back</p> Signup and view all the answers

    What condition is characterized by the inability to properly react or engage with the environment in animals?

    <p>Slow or lack of reaction</p> Signup and view all the answers

    Which symptom can indicate dehydration in laboratory animals?

    <p>Loss of appetite</p> Signup and view all the answers

    What are potential respiratory symptoms caused by severe Mycoplasma infections?

    <p>Pneumonia and rhinitis</p> Signup and view all the answers

    What is a potential general symptom related to feed and water uptake in sick animals?

    <p>Weight loss</p> Signup and view all the answers

    Which symptom is commonly associated with severe infections, such as those caused by Mycoplasma?

    <p>Oedematous swelling on ears and eyes</p> Signup and view all the answers

    What type of implantation occurs in humans?

    <p>Interstitial implantation</p> Signup and view all the answers

    During amniogenesis, how does the amnion develop in mice?

    <p>By cavitation and plectamnion</p> Signup and view all the answers

    Which term describes the separation of the inner cell mass into the embryo and amnion in humans?

    <p>Schizamnion</p> Signup and view all the answers

    What type of implantation is characteristic of pigs?

    <p>Superficial implantation</p> Signup and view all the answers

    What crucial step occurs prior to the folding that leads to plectamnion in rodents?

    <p>Hollowing of the pro-amniotic cavity</p> Signup and view all the answers

    Which of the following correctly identifies the two major types of amniogenesis?

    <p>Schizamnion and plectamnion</p> Signup and view all the answers

    In mammals, what surrounds the embryo or fetus within the amniotic cavity?

    <p>Amnion</p> Signup and view all the answers

    What is the fate of the ectoplacental cavity during amniogenesis in rodents?

    <p>It merges with the amniotic cavity</p> Signup and view all the answers

    What defines the placental barrier in terms of its classification?

    <p>The number of tissue layers nutrients must cross</p> Signup and view all the answers

    Which type of placenta is characterized by having six tissue layers?

    <p>Epitheliochorial</p> Signup and view all the answers

    In which species is the epitheliochorial type of placental barrier found?

    <p>Horse</p> Signup and view all the answers

    How many layers were identified in the human placenta, according to the Grosser classification?

    <p>Two layers</p> Signup and view all the answers

    What unique characteristic is observed in the pig's placenta regarding its capillaries?

    <p>They nestle between the epithelial cells</p> Signup and view all the answers

    The term 'haemomonochorial' indicates what about the placenta?

    <p>One layer is separated</p> Signup and view all the answers

    Which placental classification indicates a totally non-invasive placenta?

    <p>Epitheliochorial</p> Signup and view all the answers

    What phenomenon has been suggested to characterize the placenta in pigs?

    <p>Potential endothelioendothelial type</p> Signup and view all the answers

    What is the primary site where villi are found in the pig placenta?

    <p>At the site of the areolae</p> Signup and view all the answers

    Which type of placenta does NOT have microvilli present?

    <p>Endotheliochorial placenta</p> Signup and view all the answers

    What characterizes the areolae in the pig placenta?

    <p>Increased glandular activity</p> Signup and view all the answers

    Which statement is FALSE regarding paraplacental structures?

    <p>They contribute to hemotrophic nutrition.</p> Signup and view all the answers

    Which function is primarily associated with areolae in the pig placenta?

    <p>Transfer of iron</p> Signup and view all the answers

    Which of the following is NOT a characteristic used to classify placentas?

    <p>Fetal gut development</p> Signup and view all the answers

    What is a primary function of paraplacental structures?

    <p>Histiotrophic nutrition</p> Signup and view all the answers

    Which characteristic about fetal cells is correct regarding the endothelial interface?

    <p>Fetal cells do not form microvilli.</p> Signup and view all the answers

    Which of the following species belongs to the family Leporidae?

    <p>Oryctolagus cuniculus</p> Signup and view all the answers

    What is the correct sequence in the taxonomic hierarchy for Canis lupus?

    <p>Phylum: Mammalia, Order: Carnivora, Family: Canidae, Genus: Canis, Species: lupus</p> Signup and view all the answers

    Which of the following is NOT classified under the class Mammalia?

    <p>Zebrafish</p> Signup and view all the answers

    Which family does the Rhesus monkey belong to?

    <p>Cercopithecidae</p> Signup and view all the answers

    What is the species epithet of Ovis aries?

    <p>aries</p> Signup and view all the answers

    Which of these genera is associated with the family Muridae?

    <p>Mus</p> Signup and view all the answers

    Identify the correct binomen for the guinea pig.

    <p>Cavia procellus</p> Signup and view all the answers

    Which order does Ovis aries belong to?

    <p>Ruminantia</p> Signup and view all the answers

    Which class includes both fish and mammals?

    <p>Chordata</p> Signup and view all the answers

    Which of the following species is classified under the family Caviidae?

    <p>Cavia procellus</p> Signup and view all the answers

    How can you prevent wear on your needle holder?

    <p>Hold the needle closer to the tip</p> Signup and view all the answers

    Which of the following is a sedative?

    <p>Atropine</p> Signup and view all the answers

    What is correct?

    <p>A female mouse will abort if exposed to male urine within 24 hours (Bruce effect).</p> Signup and view all the answers

    What epitopes are necessary to produce antibodies?

    <p>B and T epitopes</p> Signup and view all the answers

    Why do we use a linear model to estimate the exposure risk at low doses?

    <p>To avoid underestimation.</p> Signup and view all the answers

    What is the correct statement for bipolar electrosurgery?

    <p>Bipolar electrosurgery can only be used for coagulation, not incisions.</p> Signup and view all the answers

    Which of the following has the clearest sedative capacities?

    <p>Acepromazine (ACP)</p> Signup and view all the answers

    What should never be added simultaneously with tetracyclines?

    <p>Warfarin</p> Signup and view all the answers

    To induce a specific IgG response, an antigen needs:

    <p>B and T cell epitopes</p> Signup and view all the answers

    What is allodynia?

    <p>Sensation of pain coming from a stimulus that should not be painful.</p> Signup and view all the answers

    How do you maintain the lifespan of a needle holder?

    <p>Use a needle holder with a longitudinal groove.</p> Signup and view all the answers

    Which of the following statements concerning pheromones in mouse reproduction is true?

    <p>A male mouse will urinate on a female mouse before mating.</p> Signup and view all the answers

    What is the Bruce effect?

    <p>A female mouse has an abortion within the first days of her pregnancy if she comes in close contact with male urine.</p> Signup and view all the answers

    Which type of euthanasia is not allowed for a conscious mouse?

    <p>CO2 inhalation.</p> Signup and view all the answers

    What is not allowed in Belgium?

    <p>Experiments on animals for tobacco research.</p> Signup and view all the answers

    How do you best protect the needle holdeR?

    <p>Use a needle holder with a thick tip for thick needles.</p> Signup and view all the answers

    What is the best sedative?

    <p>ACP</p> Signup and view all the answers

    What is the best excretion method for removing a drug from the body besides glomerular filtration?

    <p>Tubular secretion.</p> Signup and view all the answers

    Which statement is correct?

    <p>The premolars and molars of a rabbit grow constantly.</p> Signup and view all the answers

    Extrapolation of low radiation doses: Which statement is correct?

    <p>A linear approach is used to avoid underestimating the risk.</p> Signup and view all the answers

    Explain the 3Rs and give examples.

    <p>The 3Rs stand for Replacement, Reduction, and Refinement.</p> <ul> <li>Replacement means using alternatives to animals, such as in vitro methods, computer modeling, or human tissue cultures, whenever possible.</li> <li>Reduction involves using fewer animals or obtaining more information from the same number of animals. This could involve refining experimental design or using more sensitive and precise measurement techniques.</li> <li>Refinement aims to minimize pain and distress for animals. This can involve using better anesthetic techniques, providing more enriching environments, or ensuring proper husbandry practices.</li> </ul> Signup and view all the answers

    Describe the four basic principles of anesthesia and provide an example of medication for each.

    <p>The four basic principles of anesthesia are: Induction, Maintenance, Analgesia, and Reversal.</p> <ul> <li>Induction is the process of initiating anesthesia, usually by administering a drug intravenously or inhaling a gas. Examples of induction agents include propofol and isoflurane.</li> <li>Maintenance involves keeping the animal at a constant level of anesthesia throughout the procedure. This can be achieved by continuous administration of a drug. An example is isoflurane for inhalation anesthesia.</li> <li>Analgesia aims to relieve pain, which is often a component of anesthesia. Opioids such as morphine or fentanyl could be given for analgesia.</li> <li>Reversal is the process of reversing anesthesia, allowing the animal to wake up quickly and safely. Reversal agents are given to counteract the effects of the induction agent, such as Atipamezole for the reversal of dexmedetomidine.</li> </ul> Signup and view all the answers

    Why is it important for small laboratory animals to awaken quickly from anesthesia?

    <p>It is important for small laboratory animals to awaken quickly from anesthesia because prolonged anesthesia can lead to several complications, including hypothermia, respiratory issues, and muscle weakness. Quick recovery minimizes the risk of these problems and allows the animal to resume normal activity sooner, ensuring their well-being and the success of the experiment.</p> Signup and view all the answers

    Explain: incubation period, facultative pathogen, and subclinical carrier.

    <p>Incubation period is the time between exposure to a pathogen and the onset of clinical symptoms. A facultative pathogen is a microorganism that typically does not cause disease, but can cause illness in a weakened host or under certain conditions. A subclinical carrier is an individual who harbors a pathogen without exhibiting symptoms but can still transmit the infection to others.</p> Signup and view all the answers

    Which of the following is the most sedative?

    <p>Acepromazine</p> Signup and view all the answers

    Which imaging techniques are functional?

    <p>Scintigraphy</p> Signup and view all the answers

    What is the advantage of MRI and CT?

    <p>MRI provides detailed information about soft tissue structures, while CT is more effective for bone imaging.</p> Signup and view all the answers

    What is the main advantage of MRI and CT, in comparison to conventional radiography?

    <p>MRI and CT provide more detailed images, as opposed to a flat 2D image.</p> Signup and view all the answers

    Why does a linear model instead of a quadratic model fit the last slide of the lecture better?

    <p>The linear model is more conservative, and it is used to avoid underestimating the potential risk.</p> Signup and view all the answers

    Compare the dentition of rabbits and rats: provide the dental formula, total number of teeth, and which grow continuously.

    <p>Rabbits have a dental formula of 2I 1/1, 3P 2/1 3M 3/3 and a total of 28 teeth. Their incisors and molars grow continuously, while premolars are diphyodont, meaning they are replaced once. Rats have a dental formula of 1I 1/1, 0P 0/0 3M 3/3 and a total of 16 teeth. Their incisors grow continuously. Molars are brachyodont, meaning they stop growing once they reach their full size.</p> Signup and view all the answers

    What is the difference between physiological and pathological pain?

    <p>Physiological pain is felt in proportion to the intensity of the stimulus, while pathological pain is often disproportionate to the stimulus.</p> Signup and view all the answers

    What are the different phases of anesthesia?

    <p>The phases of anesthesia are: premedication, induction, maintenance, and recovery.</p> <ul> <li>Premedication involves administering drugs to prepare the animal for anesthesia, reduce stress, and minimize side effects.</li> <li>Induction refers to the process of initiating anesthesia, usually by administering a drug intravenously or inhaling a gas.</li> <li>Maintenance involves keeping the animal at a constant level of anesthesia throughout the procedure. This can be achieved by continuous administration of a drug.</li> <li>Recovery is the process of the animal regaining wakefulness after the effects of anesthesia have worn off.</li> </ul> Signup and view all the answers

    Study Notes

    Course Information

    • Course: Advanced Plant Cell Biology and Signaling | Master Biomedical and Plant Biotechnology
    • Academic Year: 2020-2021
    • Answers LAS Exam Questions

    Content

    • Introduction to laboratory animal science (Prof. dr. K. Hermans)
    • Postmortem examination (Prof. dr. K. Hermans)
    • Embryology (Prof. dr. P. Cornillie)
    • Diseases and disease control (Prof. dr. K. Hermans)
    • Taxonomy (Prof. dr. P. Cornillie)
    • Reproduction and reproductive techniques (Prof. dr. A. Van Soom)
    • Comparative morphology (Prof. dr. W. De Spiegelaere)
    • Principles of surgery in experimental animals (Prof. dr. A. Martens)
    • Nutrition of laboratory animals (Prof. dr. G. Janssens)
    • Basic principles of pharmacokinetics and pharmacodynamics (Prof. dr. M. Devreese)
    • Comparative physiology (Drs. C. De Meeûs)
    • Immunology (Prof. dr. E. Cox)
    • Postoperative care (Prof. dr. K. Hermans)
    • Experimental techniques (Prof. dr. K. Hermans)
    • Chapter 15: Medical Imaging
    • Chapter 16: Radiobiology and radioprotection
    • Chapter 17: Analgesia and anaesthesia
    • Chapter 18: Laboratory animal legislation
    • Chapter 19: Ethical committees
    • Chapter 20 & 21: Ethology & stress: Animal welfare, Housing.
    • Chapter 22: Environmental enrichment
    • Chapter 23: Statistics
    • Topic 24: Extrapolation of experimental data to man
    • Topic 25: The ethics of animal use
    • Topic 26: Safety in working with laboratory animals
    • Topic 27: Viral zoonoses
    • Topic 28: Bacterial zoonoses
    • Topic 29: Euthanasia of laboratory animals
    • Topic 30: Transportation of laboratory animals
    • Disease prevention & hygiene
    • GLP
    • Genetics

    Exam Preparation LAS H1-14

    • 3Rs: Replacement, Reduction, Refinement
    • Replacement: methods avoiding animal use (e.g. in vitro models)
    • Reduction: methods for reducing the number of animals or getting more information from fewer animals
    • Refinement: methods improving animal welfare
    • Non-technical summary (NTS): meant for the general public, explaining benefits of the project, how many animals are needed, and how they're treated
    • Types of animals: Category A, B, C, D (caretakers, experimenters, directors, specialists)

    Introduction in Laboratory Animal Science

    • Three Rs
    • Replacement (e.g., in vitro models, computerized models)
    • Reduction (e.g., animal sharing, improved statistical design)
    • Refinement (e.g., better techniques/equipment)
    • NTS (Non-Technical Summary)
    • Explanation of 3R aspects

    Postmortem Examination

    • Sample conservation and shipment
    • Formalin-fixed tissue preservation
    • Use 10% neutral buffered formalin
    • Use 10x more fixative than tissue
    • Steps in Postmortem examination
    • External & macroscopic evaluation of organs
    • Microscopy of gut content
    • Cytology
    • Histology
    • Bacteriology
    • Virology

    Embryology

    • Species-specific differences (placentation, organogenesis)
    • Critical period of organogenesis = Most vulnerable time for malformations
    • Teratogens = factors causing malformations
    • Limb development critical periods: Man(wk 4-7), Cat(wk 4-6), Dog(wk 3-4), Rabbit/Rat(wk 2-3)

    Diseases and Disease Control

    • Impact of disease on experimental results (subclinical carriers)
    • Incubation period
    • Facultative pathogens
    • Diseases of Pasteurella multocida in rabbits (e.g., exophthalmia, conjunctivitis, rhinitis)
    • Importance on animal welfare, transmission of diseases from animals to humans (zoonotic)
    • Epizootic
    • Enzootic
    • Quarantine
    • Subclinical Carriers

    Comparative Morphology

    • 5 major anatomical differences between rabbits and rodents (e.g., teeth, stomach, respiratory system, limbs, etc.)
    • Explanation of vertebral formula
    • Differences in body surface area across species

    Reproduction and Reproductive Techniques

    • Difference between copulatory plug/post-ovulatory discharge (different species, e.g., golden hamster)
    • Breeding schemes pros/cons
    • Effects of pheromones
    • Bruce effect

    Additional Topics (Chapters 15-30)

    • These chapters cover additional topics related to laboratory animal science, including Specific and/or general examples
    • Specific examples refer to the type of animal being used, and general examples applies to all
    • Explanations, descriptions, or types of methods

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    Description

    Test your knowledge on the health management and disease diagnosis in lab animals. This quiz covers common symptoms, treatment options, and behavioral indicators associated with various diseases. Ideal for those studying veterinary science or animal care.

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