Podcast
Questions and Answers
What is the former name of AST?
What is the former name of AST?
Which enzyme catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate?
Which enzyme catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate?
What is the optimal pH range for AST activity?
What is the optimal pH range for AST activity?
Where is the major tissue source of ALT?
Where is the major tissue source of ALT?
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What is the diagnostic significance of AST?
What is the diagnostic significance of AST?
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Which enzyme is involved in the transfer of an amino group between aspartate and alpha-keto acids?
Which enzyme is involved in the transfer of an amino group between aspartate and alpha-keto acids?
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What is the stability of AST at refrigerated temperature?
What is the stability of AST at refrigerated temperature?
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Which enzyme catalyzes the formation of oxaloacetate and glutamate?
Which enzyme catalyzes the formation of oxaloacetate and glutamate?
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Study Notes
AST (Aspartate Transaminase)
- Formerly known as SGOT (Serum Glutamic Oxaloacetic Transaminase)
Enzymatic Reactions
- AST catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate
- AST is involved in the transfer of an amino group between aspartate and alpha-keto acids
- ALT (Alanine Transaminase) catalyzes the transfer of an amino group from alanine to alpha-ketoglutarate
Activity and Stability
- Optimal pH range for AST activity is 7.4-7.8
- AST is unstable at refrigerated temperature, with a significant loss of activity within 24 hours
Diagnostic Significance
- AST is a diagnostic marker for liver damage and disease, particularly in conjunction with ALT
Tissue Sources
- Major tissue source of ALT is the liver, with smaller amounts found in the kidneys, heart, and skeletal muscles
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Description
Learn about enzyme assay specifically focusing on transaminase in the context of MLS111B laboratory activities. Understand how enzymes accelerate biochemical reactions, their presence in serum post cellular injury, and the functions of AST/SGOT. Dive into the transfer of amino groups between molecules and the formation of oxaloacetate.
