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Questions and Answers
Alanine aminotransferase (ALT) or ______ pyruvate transaminase (GPT) catalyses the reversible transfer of an amino group from alanine to α-ketoglutarate forming glutamate and piruvate.
Alanine aminotransferase (ALT) or ______ pyruvate transaminase (GPT) catalyses the reversible transfer of an amino group from alanine to α-ketoglutarate forming glutamate and piruvate.
Glutamate
The piruvate produced is reduced to ______ by lactate dehydrogenase (LDH) and NADH.
The piruvate produced is reduced to ______ by lactate dehydrogenase (LDH) and NADH.
lactate
R 1 Buffer ______ pH 7.8 is a reagent used in the method.
R 1 Buffer ______ pH 7.8 is a reagent used in the method.
TRIS
L-Alanine is a ______ in the reaction.
L-Alanine is a ______ in the reaction.
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The unit of ALT calculated is ______ per liter.
The unit of ALT calculated is ______ per liter.
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What is the pH of the Buffer used in the method?
What is the pH of the Buffer used in the method?
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What is the byproduct of the reversible transfer of an amino group from alanine to α-ketoglutarate?
What is the byproduct of the reversible transfer of an amino group from alanine to α-ketoglutarate?
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What is the enzyme that reduces piruvate to lactate?
What is the enzyme that reduces piruvate to lactate?
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What is the calculation used to determine the units of ALT?
What is the calculation used to determine the units of ALT?
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What is the role of NADH in the reaction?
What is the role of NADH in the reaction?
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