Lab -7 Enterobacteriaceae: Classification and Characteristics

Enterobacteriaceae is a large diverse family of bacteria commonly referred to as the Gram-negative ______

The genera containing the species most virulent for humans (true pathogens) are: Escherichia, Shigella, Salmonella, Klebsiella, and ______

Other less common but medically important genera are: Enterobacter, Serratia, Proteus, Morganella, and ______

All species ferment the sugar glucose but otherwise vary widely in their biochemical ______

Culture media commonly used in primary isolation to speed separation of the many species include MacConkey agar for detecting ______ fermenters and non-lactose fermenters

Tests for identification of members of Enterobacteriaceae family include IMViC test, Triple Sugar Iron (TSI) Agar Test, Urease Test, Oxidase Test, and ______ test

Escherichia coli strains ferment lactose rapidly and produce ______

Klebsiella spp. are distinctive for the absence of ______ and the presence of a polysaccharide capsule

Salmonella usually produce non-hemolytic smooth white colonies on Blood Agar, which is a non-selective media for primary isolation. Salmonella-Shigella (SS) Agar is used for selective recovery of Salmonella from stool culture, and Salmonellae usually produce lactose non-fermenting colonies with black centers. This is an example of selective or differential ______

Yersinia spp. are morphologically ______, with mucoid colonies that are small (1-2 mm diameter), flat, colorless, or pale pink colonies. Yersinia tend to be ______

Shigella species are closely related to E coli but are non-lactose fermenters. Shigella do not ferment lactose or produce hydrogen sulfide gas, so the resulting colonies will be ______. Shigella on SS agar will appear as ______ colonies

Proteus mirabilis is the most commonly isolated member of the group. P mirabilis and Proteus vulgaris share the ability to ______ over the surface of media, rather than remaining confined to discrete colonies. This is known as ______