6 Questions
What is the advantage of using a closed-tube system in Q-PCR?
It prevents carry-over contamination
What is the purpose of performing melting curve analysis in Q-PCR?
To determine the quality of the synthesized PCR products
How many samples can be diagnosed in 1 hour using Q-PCR?
32
What is the purpose of using TaqMan probes in Q-PCR?
To discriminate between allelic variants
What is the concentration of DNA in the final reaction mixture?
40 ng/µl
What is the temperature of the initial denaturation step in Q-PCR?
95 °C
This quiz explores the laboratory diagnosis of Cystic Fibrosis disease using Q-PCR combined with melting curve analysis. It covers the advantages of this method, including faster detection and prevention of carry-over contamination. The quiz also discusses the quality control of synthesized PCR products and the feasibility of multiplexing.
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