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Questions and Answers
What is the advantage of using a closed-tube system in Q-PCR?
What is the advantage of using a closed-tube system in Q-PCR?
- It prevents carry-over contamination (correct)
- It reduces the detection time to 30 minutes
- It increases the quality of the synthesized PCR products
- It allows for multiplexing
What is the purpose of performing melting curve analysis in Q-PCR?
What is the purpose of performing melting curve analysis in Q-PCR?
- To determine the quality of the synthesized PCR products (correct)
- To increase the speed of detection
- To reduce the number of primer-probe systems needed
- To detect the presence of mutations
How many samples can be diagnosed in 1 hour using Q-PCR?
How many samples can be diagnosed in 1 hour using Q-PCR?
- 40
- 24
- 32 (correct)
- 16
What is the purpose of using TaqMan probes in Q-PCR?
What is the purpose of using TaqMan probes in Q-PCR?
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What is the concentration of DNA in the final reaction mixture?
What is the concentration of DNA in the final reaction mixture?
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What is the temperature of the initial denaturation step in Q-PCR?
What is the temperature of the initial denaturation step in Q-PCR?
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