Lab 6: Cystic Fibrosis Diagnosis using Q-PCR
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Questions and Answers

What is the advantage of using a closed-tube system in Q-PCR?

  • It prevents carry-over contamination (correct)
  • It reduces the detection time to 30 minutes
  • It increases the quality of the synthesized PCR products
  • It allows for multiplexing
  • What is the purpose of performing melting curve analysis in Q-PCR?

  • To determine the quality of the synthesized PCR products (correct)
  • To increase the speed of detection
  • To reduce the number of primer-probe systems needed
  • To detect the presence of mutations
  • How many samples can be diagnosed in 1 hour using Q-PCR?

  • 40
  • 24
  • 32 (correct)
  • 16
  • What is the purpose of using TaqMan probes in Q-PCR?

    <p>To discriminate between allelic variants</p> Signup and view all the answers

    What is the concentration of DNA in the final reaction mixture?

    <p>40 ng/µl</p> Signup and view all the answers

    What is the temperature of the initial denaturation step in Q-PCR?

    <p>95 °C</p> Signup and view all the answers

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