Real Time PCR (qPCR) Essentials

To detect fluorescence in the tubes during the run

To emit fluorescence signal upon degradation by Taq polymerase

Coating microwell plates with antigen and quantitating labeled antibody binding

<p>Emits prominent fluorescent signal when it binds to dsDNA</p> Signup and view all the answers

<p>Detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones</p> Signup and view all the answers

<p>To emit a detectable fluorescence signal upon degradation by Taq polymerase</p> Signup and view all the answers

<p>To emit a prominent fluorescent signal when it binds to dsDNA</p> Signup and view all the answers

<p>To detect fluorescence in the tubes during run</p> Signup and view all the answers

<p>Detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones</p> Signup and view all the answers

<p>Microwell plates coated with antigen and quantitation of binding labeled antibody by colorimetric method</p> Signup and view all the answers