Real Time PCR (qPCR) Essentials
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Questions and Answers

What is the purpose of the optical module in real time PCR system?

  • To conjugate with a reporter fluorochrome
  • To detect fluorescence in the tubes during the run (correct)
  • To amplify and quantify a target DNA molecule
  • To translate fluorescence data into result

    • What is the function of TaqMan probe in real time PCR?

  • To bind to dsDNA and emit a fluorescent signal
  • To prevent the amplification of target DNA molecules
  • To emit fluorescence signal upon degradation by Taq polymerase (correct)
  • To detect and quantify soluble substances

    • In ELISA, what does a direct ELISA involve?

  • Coating microwell plates with antigen and quantitating labeled antibody binding (correct)
  • Conjugating a reporter fluorochrome with a quencher fluorochrome
  • Detecting and quantifying soluble substances such as peptides and proteins
  • Using TaqMan probe to emit fluorescence signal

    • What is the function of SYBR Green in real time PCR?

    <p>Emits prominent fluorescent signal when it binds to dsDNA</p> Signup and view all the answers

    What is the main purpose of ELISA (enzyme-linked immunosorbent assay)?

    <p>Detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones</p> Signup and view all the answers

    What is the function of TaqMan probe in real time PCR?

    <p>To emit a detectable fluorescence signal upon degradation by Taq polymerase</p> Signup and view all the answers

    What is the main purpose of SYBR Green in real time PCR?

    <p>To emit a prominent fluorescent signal when it binds to dsDNA</p> Signup and view all the answers

    What is the function of the optical module in real time PCR system?

    <p>To detect fluorescence in the tubes during run</p> Signup and view all the answers

    What is the main purpose of ELISA (enzyme-linked immunosorbent assay)?

    <p>Detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones</p> Signup and view all the answers

    What does a direct ELISA involve?

    <p>Microwell plates coated with antigen and quantitation of binding labeled antibody by colorimetric method</p> Signup and view all the answers

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