Lab 1: Carbohydrate Detection Tests
30 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the general composition of carbohydrates?

  • Carbon, hydrogen, and helium
  • Carbon, oxygen, and nitrogen
  • Carbon, oxygen, and hydrogen (correct)
  • Oxygen, hydrogen, and nitrogen

Which functional group is present in carbohydrates?

  • Ether functional group
  • Aldehydic or ketonic functional group (correct)
  • Carboxylic acid functional group
  • Amine functional group

What is the purpose of Molisch's Test?

  • To detect proteins in a solution
  • To detect nucleic acids in a solution
  • To detect carbohydrates in a solution (correct)
  • To detect lipids in a solution

What is the reaction underlying Molisch's Test?

<p>Formation of Furfural and its derivatives (B)</p> Signup and view all the answers

What is the purpose of Fehling's Test?

<p>To detect the presence of reducing sugars and non-reducing sugars (B)</p> Signup and view all the answers

Who developed the Fehling's Test?

<p>H.C. Von Fehling (D)</p> Signup and view all the answers

What is the typical colour of Fehling's A solution?

<p>Blue (C)</p> Signup and view all the answers

What is the role of tartrate tetra-anions in Fehling's solution?

<p>Chelating agent (D)</p> Signup and view all the answers

How are Fehling's A and B solutions mixed to prepare the final Fehling solution?

<p>In equal volumes (B)</p> Signup and view all the answers

What is the purpose of the control tube in the Fehling's test?

<p>To contain distilled water (B)</p> Signup and view all the answers

What is the expected outcome of the Fehling's test?

<p>Formation of a red precipitate (A)</p> Signup and view all the answers

In which condition are the tubes containing the sample and Fehling's solution placed?

<p>In a water bath (B)</p> Signup and view all the answers

What is the primary purpose of the Biuret test?

<p>To demonstrate the presence of the peptide bond (A)</p> Signup and view all the answers

What is the expected outcome of the Iodine test if starch is present?

<p>A blue-black colored complex (B)</p> Signup and view all the answers

What is the purpose of the control sample in the Iodine test?

<p>To compare the results with the test sample (C)</p> Signup and view all the answers

What is the initial step in the Biuret test procedure?

<p>Add 2 cm3 of the liquid food sample to a clean, dry test tube (B)</p> Signup and view all the answers

What is the effect of heating the test tubes in the Iodine test?

<p>The color that develops in the test tubes disappears (B)</p> Signup and view all the answers

What is the quantity of the liquid sample required for the Iodine test?

<p>1 ml (A)</p> Signup and view all the answers

What is the indicator of a positive Benedict's test?

<p>Formation of a reddish brown precipitate (B)</p> Signup and view all the answers

What is the purpose of heating the solution in a boiling water bath during Benedict's test?

<p>To facilitate the reaction between the sample and Benedict's reagent (D)</p> Signup and view all the answers

Which of the following sugars does not give a positive Benedict's test?

<p>Sucrose (A)</p> Signup and view all the answers

What is the function of CuSO4 in Benedict's test?

<p>To act as an indicator for the presence of reducing sugars (C)</p> Signup and view all the answers

What is the purpose of Barfoed's test?

<p>To distinguish between monosaccharides and disaccharides (A)</p> Signup and view all the answers

What happens to sucrose when it undergoes Barfoed's test?

<p>It undergoes hydrolysis in the presence of an acid (D)</p> Signup and view all the answers

What is the primary purpose of Barfoed's test?

<p>To differentiate between monosaccharides and disaccharides (A)</p> Signup and view all the answers

What is the role of the acidic medium in Barfoed's test?

<p>It enhances the reduction of cupric ions to cuprous ions (C)</p> Signup and view all the answers

What is the outcome of the test if red precipitates are formed after the initial 5 minutes?

<p>The presence of a monosaccharide is indicated (C)</p> Signup and view all the answers

Why is it necessary to allow the solution to cool gradually at room temperature?

<p>To prevent the decomposition of the test solution (D)</p> Signup and view all the answers

What happens to disaccharides when the heating period is increased?

<p>They are converted to monosaccharides (D)</p> Signup and view all the answers

What is the name of the test that is often used to detect the presence of protein in a given solution?

<p>Biuret test (D)</p> Signup and view all the answers
Use Quizgecko on...
Browser
Browser