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Questions and Answers
What is the expected result of the Seliwanof’s test for fructose?
What is the expected result of the Seliwanof’s test for sucrose?
What is the expected result of the Seliwanof’s test for glucose?
What is the expected result of the Seliwanof’s test for lactose?
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What is the minimum concentration of fructose (ketose) the Seliwanof’s test can detect?
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What is the expected result of the Seliwanof’s test for a disaccharide containing both glucose and fructose?
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In the Seliwanof’s test, what is the expected result for a monosaccharide that is an aldose?
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What is the expected result of the Seliwanof’s test for a disaccharide that contains only glucose?
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Which monosaccharide is expected to take the longest time to give a positive result in the Seliwanof’s test?
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If a sample gives a positive Seliwanof’s test result after more than 20 minutes, what type of sugar(s) could be present in the sample?
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Which disaccharide would be expected to give a positive Seliwanof’s test result after more than 20 minutes?
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If a sample gives a positive Seliwanof’s test result after 6 minutes, what type of sugar(s) could be present in the sample?
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Which monosaccharide is expected to take the longest time to give a positive result in the Seliwanof’s test?
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What is the minimum concentration of fructose (ketose) the Seliwanof’s test can detect?
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Which disaccharide is expected to be digested to monosaccharides of fructose and then dehydrate to give a positive result in the Seliwanof’s test after 6 minutes?
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Study Notes
Seliwanoff's Test
- Used to differentiate between ketoses and aldoses in mono and disaccharides.
- Employs HCl as a dehydrating agent and resorcinol as a condensing agent.
- Produces a clear colored solution with intensity proportional to the amount of ketoses.
- Can be coupled with spectrophotometric measurement to quantify the amount of ketoses in a solution.
Reaction Principle
- Involves a dehydration reaction due to the hydroxyl groups of the sugar.
- Seliwanoff's reagent consists of resorcinol in dilute hydrochloric acid.
- Ketoses (e.g., fructose) are more readily dehydrated by HCl than aldoses, forming hydroxymethyl furfural.
- Hydroxymethyl furfural condenses with resorcinol to form a red-colored complex.
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Description
Test your knowledge of Seliwanoff’s test, used in biochemistry to differentiate between ketoses and aldoses in mono and disaccharides. Learn about the reagents used and the specific properties of the reaction.
