Histopathology & MedTech Laws Pre-Board (2 Corinthians 12:9)

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Questions and Answers

The first and most critical step in histotechnology:

  • Fixation (correct)
  • Clearing
  • Infiltration
  • Embedding

Formaldehyde is commercially supplied as:

  • 5% solution
  • 10% solution
  • 20% solution
  • 37 to 40% solution (correct)

10% formalin is the same as:

  • 37% to 40% formaldehyde
  • 10% formaldehyde
  • 4% formaldehyde (correct)
  • 1% formaldehyde

It is recommended for fixing chromosomes, lymph glands and urgent biopsies:

<p>Carnoy's fluid (B)</p> Signup and view all the answers

An excellent fixative for electron microscopy:

<p>Glutaraldehyde (B)</p> Signup and view all the answers

What are the processes done by the automatic tissue processor?

<p>Fixation, dehydration, clearing and infiltration (C)</p> Signup and view all the answers

Enclosed tissue processor:

<p>Fluid transfer (A)</p> Signup and view all the answers

Tissue is soft when block is trimmed:

<p>Incomplete fixation (A)</p> Signup and view all the answers

Xylene turns milky as soon as tissue is placed in it:

<p>Incomplete dehydration (B)</p> Signup and view all the answers

Tissue is opaque, section cutting is difficult due to the presence of alcohol.

<p>Incomplete clearing (C)</p> Signup and view all the answers

Air holes found during trimming:

<p>Incomplete impregnation (D)</p> Signup and view all the answers

Paraffin sections are usually cut in the microtome for routine histologic procedures with a thickness of:

<p>4 to 6 micra (C)</p> Signup and view all the answers

Temperature of the flotation water bath:

<p>45 to 50 °C (B)</p> Signup and view all the answers

These knives are used to cut block for ELECTRON MICROSCOPY (EM):

<p>Diamond or glass knives (A)</p> Signup and view all the answers

Microtome knives for PARAFFIN SECTIONING are usually made of:

<p>Carbon steel (A)</p> Signup and view all the answers

Sections fail to form ribbons:

  1. Horizontal surface of the block is not parallel to the knife
  2. Knife is tilted too much, knife is dull
  3. Paraffin wax is too hard, sections are too thick
  4. Surfaces and edges of the block are not parallel

<p>1, 2, 3 and 4 (D)</p> Signup and view all the answers

When liver is cut in the cryostat, the sections obtained are alternately thick and thin, with a distinct "venetian blind" effect. The most probable cause is that the:

<p>Knife is dull (C)</p> Signup and view all the answers

Adhesives for paraffin sections:

  1. Egg albumin
  2. Plasma
  3. Gelatin
  4. Canada balsam

<p>1, 2 and 3 (C)</p> Signup and view all the answers

When using the regressive staining method, the nucleus is deliberately overstained with:

<p>Non-acidified hematoxylin (D)</p> Signup and view all the answers

In H and E staining, pale nuclei are obtained. This indicates problem with:

<p>Hematoxylin stain (A)</p> Signup and view all the answers

Natural dyes:

  1. Hematoxylin
  2. Cochineal dyes
  3. Orcein
  4. Saffron

<p>1, 2, 3 and 4 (D)</p> Signup and view all the answers

Simplest method of differential staining of collagen using a mixture of picric acid and acid fuchsin:

<p>Van Gieson's stain (A)</p> Signup and view all the answers

RETICULIN FIBERS IN GOMORI'S silver impregnation stain:

<p>Black (A)</p> Signup and view all the answers

The PHOSPHOTUNGSTIC ACID HEMATOXYLIN (PTAH) is useful for demonstrating:

<p>Muscle striations (B)</p> Signup and view all the answers

Most sensitive of the lipid stains:

<p>Sudan Black B (D)</p> Signup and view all the answers

Stain for NEURONS, AXONS AND NEUROFIBRILS:

<p>Bielschowsky's technique (A)</p> Signup and view all the answers

Stain for demonstration of myelin:

<p>Luxol fast blue (B)</p> Signup and view all the answers

Stain for COPPER:

<p>Lindquist's modified rhodanine technique (B)</p> Signup and view all the answers

Acridine orange demonstrates:

<p>Nucleic acid (D)</p> Signup and view all the answers

Most reliable and specific histochemical staining technique for DNA:

<p>Feulgen reaction (A)</p> Signup and view all the answers

Stain for demonstration of Leprosy bacilli and Nocardia:

<p>Wade-Fite (C)</p> Signup and view all the answers

Stain for demonstration of Helicobacter pylori:

<p>Toluidine blue (B)</p> Signup and view all the answers

Warthin-Starry stain (WS) is a silver nitrate-based staining method (a silver stain) used in demonstration of:

<p>Spirochetes (D)</p> Signup and view all the answers

Retention of paraffin blocks:

<p>10 years (D)</p> Signup and view all the answers

Stain of choice for paraffin sections:

<p>H and E stain (C)</p> Signup and view all the answers

Stain of choice for exfoliative cytology:

<p>Original Pap's stain (C)</p> Signup and view all the answers

Most commonly used fixative for cytology:

<p>95% ethyl alcohol (C)</p> Signup and view all the answers

Father of modern cytopathology:

<p>George Nicholas Papanicolaou (C)</p> Signup and view all the answers

AMINOETHYLCARBAZOLE (AEC), which is ____ in color, is a common chromogen for peroxidases.

<p>Red (D)</p> Signup and view all the answers

DIAMINOBENZIDINE (DAB), which is ____ in color, is a common chromogen for peroxidases.

<p>Brown (A)</p> Signup and view all the answers

Antigens that are expressed by normal cells as well as tumor cells.

<p>Tumor-associated antigens (TAA) (B)</p> Signup and view all the answers

Antigens that are unique to the tumor of an individual patient or shared by a limited number of patients with the same type of tumor.

<p>Tumor-specific antigens (TSA) (C)</p> Signup and view all the answers

Which of the following is a marker used in immunohistochemical staining to identify tumors of epithelial origin?

<p>Cytokeratins (C)</p> Signup and view all the answers

Apoptosis is morphologically identified by:

<p>Nuclear condensation (B)</p> Signup and view all the answers

Thyroid follicles are lined by this type of epithelium:

<p>Simple cuboidal epithelium (B)</p> Signup and view all the answers

Elastic cartilage EXCEPT:

<p>Intervertebral discs (C)</p> Signup and view all the answers

Smooth muscle tissue:

<p>Uterus (D)</p> Signup and view all the answers

Cellular adaptation to stress:

  1. Hypertrophy
  2. Hyperplasia
  3. Atrophy
  4. Dysplasia
  5. Metaplasia

<p>1, 2, 3, 4 and 5 (D)</p> Signup and view all the answers

Cellular adaptation to stress:

<p>Dysplasia (B)</p> Signup and view all the answers

Molecular death:

<p>Rigor mortis (D)</p> Signup and view all the answers

Person authorized to perform autopsy and dissection of remains:

  1. Health officers
  2. Medical officers of law enforcement agencies
  3. Members of the medical staff of accredited hospitals

<p>1, 2 and 3 (D)</p> Signup and view all the answers

A person died of unknown disease, the physician would like to know the final diagnosis. Who is authorized to give consent for autopsy?

<p>Next of kin or the nearest relative of the deceased (C)</p> Signup and view all the answers

For all fetal deaths aged 20 weeks and above as calculated from the start of the last menstrual period to the date of delivery, which form must be prepared and the causes of death completely filled out by the certifier?

<p>Certificate of Fetal Death (C)</p> Signup and view all the answers

FORENSIC AND ANATOMIC PATHOLOGY report should be kept in the laboratory for a period of:

<p>Permanently (D)</p> Signup and view all the answers

The chairman and members of the BOARD OF MT shall hold office for ___ year(s) after appointments or until their successors shall have been appointed and duly qualified.

<p>3 years or until their successors shall have been appointed and duly qualified (C)</p> Signup and view all the answers

No further board examination will be given to an applicant who has not qualified after ___ examinations unless he completed 12 months refresher course/postgraduate training.

<p>3 (B)</p> Signup and view all the answers

Certificate of registration as a medical technologist shall be issued to successful applicants who attained the age of at least:

<p>21 years old (C)</p> Signup and view all the answers

Renewal of CLINICAL LABORATORY license:

<p>October to November (D)</p> Signup and view all the answers

Subject to RANDOM drug test:

<p>Students of secondary and tertiary schools (D)</p> Signup and view all the answers

Learning activities such as online training, local/international seminars/nondegree courses, institution/company-sponsored training programs, and the like, which did not undergo CPD accreditation but may be applied for and awarded CPD units by the respective CPD Council:

<p>Self-directed learning (D)</p> Signup and view all the answers

Failure to give reasonable care by the healthcare provider:

<p>Negligence (C)</p> Signup and view all the answers

An act done to avoid harming the patients:

<p>Non-maleficence (D)</p> Signup and view all the answers

Which management function does staffing of the laboratory fulfill?

<p>Planning (A)</p> Signup and view all the answers

In what phase of the management process does the manager gather together the necessary resources and people and develop an organizational structure to put the formal plan into action?

<p>Organizing (C)</p> Signup and view all the answers

Which management function is LEADERSHIP considered the fulfillment of:

<p>Directing (C)</p> Signup and view all the answers

Which management function is responsible for MONITORING THE STANDARDS and feedback mechanisms set in place when objectives were established during the planning stage?

<p>Controlling (D)</p> Signup and view all the answers

An order form that is sent to the laboratory along with a specimen:

<p>Specimen requisition (C)</p> Signup and view all the answers

Publisher of Good Laboratory Practice:

<p>WHO (D)</p> Signup and view all the answers

All laboratory procedures and policies must be reviewed and documented at least:

<p>Annually or when authorized changes are made (D)</p> Signup and view all the answers

Interface:

<p>Exchange of information (C)</p> Signup and view all the answers

User passwords should be:

<p>Kept confidential (B)</p> Signup and view all the answers

Which of the following is the advantage for adding POCT?

<p>Faster TAT (C)</p> Signup and view all the answers

Pre-analytical (pre-examination) variables in laboratory testing include:

<p>Specimen acceptability (D)</p> Signup and view all the answers

A pre-analytical error can be introduced by:

<p>Vigorously shaking of blood tube to prevent clotting (D)</p> Signup and view all the answers

Pre-analytical errors are major contributors to total laboratory errors. Which of the following is not a common preanalytical error?

<p>Failure to flag a critical value on a laboratory report (D)</p> Signup and view all the answers

Which of the following is not a potential source of post-analytical (post-examination) errors?

<p>Labeling the specimen at the nurses' station (D)</p> Signup and view all the answers

The most important diagnosis and therapeutic management decision tool used to interpret test results is:

<p>Reference intervals (B)</p> Signup and view all the answers

A delta check is a method that:

<p>Monitors patient samples day to day (C)</p> Signup and view all the answers

What is the first step that should be taken in response to an out-of-control condition?

<p>Discontinue patient testing/reporting (D)</p> Signup and view all the answers

The first procedure to be followed if the blood gas instrument is out-of-control for all parameters is:

<p>Recalibrate, then repeat control (A)</p> Signup and view all the answers

A mean value of 100 and a standard deviation of 1.8 mg/dl are obtained from a set of glucose measurements on a control solution. The 95% confidence interval in mg/dl would be:

<p>96.4 to 103.6 (B)</p> Signup and view all the answers

Upon completion of a run of cholesterol tests, the technician recognizes that the controls are not within the 2 standard deviations confidence range. What is the appropriate course of action?

<p>Run a new set of controls and repeat specimens (C)</p> Signup and view all the answers

A laboratory procedure manual should be available to personnel at the workbench and must include the following elements:

<p>Step-by-step performance instructions, reference intervals, reagent storage location, specimen collection requirements (B)</p> Signup and view all the answers

The precision of an instrument is validated by:

<p>Running the same sample multiple times (A)</p> Signup and view all the answers

Test precision is verified by:

<p>Comparing results from analyzing the same specimens on multiple analyzers over time (A)</p> Signup and view all the answers

The process by which an agency or organization uses predetermined standards to evaluate and recognize a program of study in an institution is called:

<p>Accreditation (C)</p> Signup and view all the answers

A general term for the formal recognition of professional or technical competence is:

<p>Credentialing (D)</p> Signup and view all the answers

Providing the performance feedback of employees should be done:

<p>In the form of immediate feedback and at regular intervals (D)</p> Signup and view all the answers

All of the following are PPE, EXCEPT:

<p>Hand cream (C)</p> Signup and view all the answers

What is the single most effective method to prevent nosocomial spread of infection?

<p>Perform frequent and appropriate hand hygiene (C)</p> Signup and view all the answers

Which order of events should be followed at the conclusion of a laboratory worker's shift in order to prevent the spread of bloodborne pathogens?

<p>Disinfect area, remove gloves, remove lab coat, wash hands (B)</p> Signup and view all the answers

Which of the following is the best choice for decontaminating bench tops?

<p>Sodium hypochlorite (A)</p> Signup and view all the answers

Infection rate is highest for laboratory professionals exposed to blood and body fluids containing:

<p>Hepatitis B (B)</p> Signup and view all the answers

After an accidental needle stick with a contaminated needle, the first action should be to:

<p>Thoroughly wash the wound with soap and water (D)</p> Signup and view all the answers

Gloves worn in the laboratory for specimen processing must be removed and hands washed when:

<p>Answering the telephone in a designated &quot;clean&quot; area (C)</p> Signup and view all the answers

To prevent injury, a safe lab work practice is to:

<p>Secure long hair and jewelry (A)</p> Signup and view all the answers

A technologist spilled concentrated hydrochloric acid on his/her clothing and skin, affecting a large portion of the body. After removing involved clothing, the next first aid treatment step would be to:

<p>Use emergency safety shower and flush body with water (B)</p> Signup and view all the answers

All sealed centrifuge buckets should be loaded and unloaded in a/an:

<p>Biological safety cabinet (D)</p> Signup and view all the answers

Promotion of health and safety of which of the following should be the primary objective in Quality and Safety Control programs adopted by the histopathology laboratories:

  1. Patients
  2. Laboratory personnel
  3. Environment

<p>1, 2 and 3 (D)</p> Signup and view all the answers

Epidemiologic triad consists of:

<p>Agent, host and environment (A)</p> Signup and view all the answers

Flashcards

Fixation

The first and most critical step in histotechnology, preserving tissue structure.

Formaldehyde Concentration

Formaldehyde is commercially supplied as a 37 to 40% solution.

10% Formalin

10% formalin is the same as 4% formaldehyde solution.

Carnoy's Fluid

Recommended for fixing chromosomes, lymph glands and urgent biopsies.

Signup and view all the flashcards

Glutaraldehyde

An excellent fixative for electron microscopy, preserving ultrastructure.

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Tissue Processor Steps

The automatic tissue processor performs fixation, dehydration, clearing, and infiltration.

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Fluid Transfer Processor

A tissue processor that moves fluids around the tissue.

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Soft Tissue Block Cause

Often due to insufficient immersion time or inadequate fixative penetration.

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Milky Xylene Cause

Indicates incomplete dehydration, with water present in the tissue.

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Opaque Tissue in Clearing

Interference during section cutting due to alcohol.

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Air Holes in Tissue

Often results from insufficient paraffin infiltration.

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Paraffin Section Thickness

Paraffin sections are typically cut at this thickness for routine histology.

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Water Bath Temperature

Temperature range for optimal tissue flattening.

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EM Knives

Knives used for ultrathin sectioning in electron microscopy.

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Paraffin Knife Material

Microtome knives for paraffin sectioning.

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Regressive Nuclear Staining

Overstained with non-acidified hematoxylin.

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Pale Nuclei in H&E

Hematoxylin stain is the issue.

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Van Gieson's Stain

Van Gieson's stain uses picric acid and acid fuchsin to differentially stain collagen.

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Gomori's Reticulin Stain Color

Reticulin fibers appear black.

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PTAH Stain Use

PTAH stains muscle striations.

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Sudan Black B Sensitivity

Most sensitive of the lipid stains, showing even small deposits.

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Bielschowsky's Technique

Stain for neurons, axons and neurofibrils.

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Luxol Fast Blue

Demonstrates myelin sheaths around nerve fibers.

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Stain for Copper

Lindquist's modified rhodanine technique.

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Acridine Orange

Acridine orange demonstrates nucleic acids.

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Specific DNA Stain

Feulgen reaction is specific for DNA.

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Wade-Fite Stain

Stain for Leprosy bacilli and Nocardia.

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Toluidine Blue

Stain for Helicobacter pylori.

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Warthin-Starry Stain

Silver stain used in demonstration of spirochetes.

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Paraffin Block Retention

10 years is the standard retention period.

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