Questions and Answers
What process involves placing small pieces of tissue on a microscopic slide and compressing them?
Decalcification is a process necessary for all types of tissues.
False
What is the purpose of fixation in histopathologic techniques?
Preservation
The technique of removing intracellular and extracellular fluid during tissue preparation is known as __________.
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Match the following histopathologic processes with their descriptions:
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What is the purpose of clearing in histopathology?
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Study Notes
Histopathologic Techniques
- Involves preparation of tissue samples for microscopic examination.
- Processes include fixation, dehydration, clearing, infiltration, embedding, trimming, sectioning, staining, and labeling.
Fixation
- Preserves tissue structure and composition.
- Particularly essential for calcified tissues, such as bones and teeth.
Decalcification
- Process that removes calcium or lime salts from tissues, necessary for examining calcified specimens.
Frozen Section
- Rapid diagnostic tool for timely evaluation of tissues, beneficial for demonstrating lipids and nervous tissue components.
Dehydration
- Involves removing intracellular and extracellular fluids from tissues through desiccation.
Clearing
- Eliminates alcohol from tissue samples post-dehydration to prepare for infiltration.
Infiltration and Embedding
- Infiltration involves impregnating tissue with a medium.
- Embedding casts or blocks the infiltrated tissues for easier sectioning.
Trimming
- Removes excess wax from embedded tissue blocks to ensure even sectioning.
- Not all tissue blocks require this step.
Sectioning
- Cutting tissue blocks into uniformly thin slices for microscopic examination.
- Uniform thickness is crucial; slices that are too thick or thin are unsuitable for analysis.
Staining
- Dyes are used to enhance visibility of tissue structures under a microscope.
Mounting
- The process of placing the prepared section onto a slide for examination.
Smearing Techniques
- Smearing is critical for cytological evaluations, notably in cancer diagnostics.
- Streaking: Quick, direct application method using an applicator stick or platinum loop for uniform distribution.
- Spreading: More detailed technique to maintain intercellular relationships, useful for mucous strands.
Examination of Tissue Types
- Fresh Tissues: Typically examined when immediate evaluation is necessary (e.g., fresh sputum, bronchial aspirates).
- Preserved Tissues: Used for situations requiring preservation of samples for later evaluation.
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Description
Test your knowledge on histopathology techniques and relevant medtech laws in this comprehensive quiz. Perfect for students in the Medical Technology Assessment Program. Challenge yourself and see how much you know about tissue processing and medical regulations.
