Podcast
Questions and Answers
What is the first step in isolating cells of a uniform type from a tissue?
What is the first step in isolating cells of a uniform type from a tissue?
Disrupting the extracellular matrix
What type of tissues usually yield the best dissociated cells?
What type of tissues usually yield the best dissociated cells?
Fetal or neonatal tissues
What type of enzymes are typically used to digest proteins in the extracellular matrix?
What type of enzymes are typically used to digest proteins in the extracellular matrix?
Proteolytic enzymes like trypsin and collagenase
What is the role of ethylenediaminetetraacetic acid (EDTA) in isolating cells?
What is the role of ethylenediaminetetraacetic acid (EDTA) in isolating cells?
How can large cells be separated from small cells in a mixed cell suspension?
How can large cells be separated from small cells in a mixed cell suspension?
What is the purpose of gentle agitation in isolating cells from tissue?
What is the purpose of gentle agitation in isolating cells from tissue?
What is the purpose of isolating cells from a tissue and growing them in culture?
What is the purpose of isolating cells from a tissue and growing them in culture?
Why do most biochemical procedures require obtaining large numbers of cells?
Why do most biochemical procedures require obtaining large numbers of cells?
Why do biologists develop ways to dissociate cells from tissues and separate various types?
Why do biologists develop ways to dissociate cells from tissues and separate various types?
What is the benefit of allowing isolated cells to proliferate as a pure culture?
What is the benefit of allowing isolated cells to proliferate as a pure culture?
How can heterogenous cell populations in a tissue be handled to analyze individual cell types?
How can heterogenous cell populations in a tissue be handled to analyze individual cell types?
What is the significance of obtaining a homogenous population of cells for analysis?
What is the significance of obtaining a homogenous population of cells for analysis?
How can cells be separated based on their tendency to adhere strongly to glass or plastic?
How can cells be separated based on their tendency to adhere strongly to glass or plastic?
What is the principle behind separating cells by antibodies coupled to various matrices?
What is the principle behind separating cells by antibodies coupled to various matrices?
How are cells recovered after being bound to antibodies coupled to matrices?
How are cells recovered after being bound to antibodies coupled to matrices?
What are some examples of matrices that can be used along with antibodies for cell separation?
What are some examples of matrices that can be used along with antibodies for cell separation?
Explain how trypsin can be used in the process of recovering cells bound to antibodies.
Explain how trypsin can be used in the process of recovering cells bound to antibodies.
What is the role of collagenase in cell separation using digestible matrices?
What is the role of collagenase in cell separation using digestible matrices?
