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Questions and Answers
Biochemistry is the application of chemistry to the study of biological processes at the _____ and _____ level.
cellular, molecular
What is the importance of water in biochemistry?
Water is crucial for the chemical reactions and physiological processes in organisms.
What are the types of nucleic acids discussed in the course?
DNA and RNA
What does the term 'Biochemistry' refer to?
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What is one method discussed for genetic engineering in plants?
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Match the following plant biotechnology techniques with their applications:
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Study Notes
Course Overview
- Course Name: Fundamentals of Plant Biochemistry and Biotechnology
- Course Code: PBPT 111
- Instructor: Dr. Santanu Mukherjee
- Credit Hours: 3 (2 lecture hours + 1 lab hour)
Course Objectives
- Explore biochemistry unique to plants.
- Understand critical biochemical pathways in plants.
- Learn regulation and metabolism of plant cellular components.
- Integrate plant sciences with molecular biology for applications in food, feed, and health.
Course Contents
- Importance of Biochemistry: Fundamental role in understanding living organisms.
- Properties of Water: Understanding pH and buffer systems critical to biological processes.
- Carbohydrates: Classification, importance, structures of monosaccharides, disaccharides, and polysaccharides; phenomena like mutarotation.
- Lipids: Classification and significance; properties of fatty acids, storage, and membrane lipids.
- Proteins: Classification and importance, amino acid structure (zwitterions), protein organization levels.
- Enzymology: General properties and classification; mechanisms including Michaelis-Menten and Lineweaver-Burk equations; introduction to allosteric enzymes.
- Nucleic Acids: Importance and classification; structural differences in DNA (A, B & Z forms) and various RNA types.
Metabolism
- Carbohydrate Metabolism: Pathways like glycolysis, TCA cycle, glyoxylate cycle, and electron transport chain.
- Lipid Metabolism: Processes such as beta-oxidation and fatty acid biosynthesis.
Plant Biotechnology Concepts
- Scope of plant biotechnology including various culture techniques: organ culture, embryo culture, anther culture, and somatic hybridization.
- Synthetic seeds and their significance in plant breeding.
- Techniques of gene transfer: methods include physical (Gene gun), chemical (PEG mediated), and Agrobacterium mediated.
- Transgenic plants and their role in crop improvement.
- Applications of PCR techniques, RFLP, RAPD, SSR, and marker-assisted breeding.
- Overview of biotechnology regulations and ethical considerations in plant research.
Learning Outcomes
- Equip students with problem-solving skills in biotechnological contexts.
- Enable understanding of secondary metabolite production and recombinant protein technology using plant cells.
Recommended Textbooks
- "Plant Biotechnology and Genetics: Principles, Techniques and Applications" by Stewart NC Jr.
- "Fundamentals of Biochemistry" by Voet, Voet & Pratt.
- "Biotechnology for Beginners" by Renneberg R.
- "Biochemistry and Molecular Biology" by William H.E. and Daphne C.E.
- "Lehninger Principles of Biochemistry" by Nelson and Cox.
- "Principles and Techniques of Biochemistry and Molecular Biology" by Wilson & Walker.
- "Molecular Biology and Biotechnology: A Guide for Teachers" by Kreuzer & Massey.
Definitions
- Biochemistry: Study of chemical and physical processes in organisms at molecular and cellular levels, applying chemistry to biology.
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Description
This quiz covers the essential concepts of plant biochemistry and biotechnology as introduced in the course PBPT 111. It aims to assess knowledge on biochemical processes unique to plants and their implications. Perfect for students looking to reinforce their understanding of plant biochemistry.
