Introduction to Chromatography

Chromatography is a laboratory technique used to separate, identify, and analyze the components of a mixture.

<p>Size-Exclusion Chromatography</p> Signup and view all the answers

<p>Analysis of pharmaceuticals, biomolecules, and environmental samples</p> Signup and view all the answers

<p>Liquid or solid coating inside a column.</p> Signup and view all the answers

<p>Components travel across a silica or alumina-coated plate at different rates based on polarity.</p> Signup and view all the answers

<p>Purification of proteins, enzymes, and antibodies.</p> Signup and view all the answers

<p>General purification of compounds.</p> Signup and view all the answers

<p>Extraction is a process used to separate a desired substance from a mixture using a solvent.</p> Signup and view all the answers

<p>Partitioning of a solute between two immiscible liquids.</p> Signup and view all the answers

<p>Using supercritical CO2 as a solvent to extract compounds.</p> Signup and view all the answers

<p>Repeated solvent reflux for thorough extraction.</p> Signup and view all the answers

<p>Ultrasonic waves enhance solvent penetration into the sample.</p> Signup and view all the answers

<p>All of the above</p> Signup and view all the answers

<p>Isolation of bioactive compounds from natural sources for drug development.</p> Signup and view all the answers

<p>Extraction can be used to obtain a crude mixture of compounds, and chromatography can then be used to purify and analyze the mixture.</p> Signup and view all the answers

<p>These techniques ensure the purity, quality, and efficacy of drugs, playing a critical role from drug discovery to manufacturing.</p> Signup and view all the answers

<p>All of the above</p> Signup and view all the answers

<p>Analysis of volatile compounds and residual solvents in pharmaceutical products.</p> Signup and view all the answers

<p>Qualitative analysis of mixtures, initial screening of drug discovery, and identification of active ingredients in pharmaceutical mixtures.</p> Signup and view all the answers

<p>Purification of therapeutic proteins, antibodies, and enzymes.</p> Signup and view all the answers

<p>Purification of ionic compounds, such as amino acids, nucleotides, viral particles, and growth hormones.</p> Signup and view all the answers

<p>Partitioning of solutes between two immiscible liquids based on their solubility.</p> Signup and view all the answers

<p>Using supercritical fluids, often CO2, as solvents to extract non-polar compounds.</p> Signup and view all the answers

<p>Repeatedly extracting solutes using a heated solvent.</p> Signup and view all the answers

<p>Ultrasonic waves enhance solvent penetration and solute dissolution.</p> Signup and view all the answers

<p>They are used to isolate and purify bioactive compounds from natural sources, such as plants and microorganisms, and to identify and purify synthetic intermediates for drug synthesis.</p> Signup and view all the answers

<p>They are used to ensure the stability and uniformity of drug formulations and to monitor the concentration of preservatives and other excipients in drug products.</p> Signup and view all the answers

<p>They are used to assess the purity and identity of active pharmaceutical ingredients, to detect impurities and degradation products, and to monitor the concentration of critical components in drug formulations.</p> Signup and view all the answers

<p>They are indispensable tools for drug development, quality control, and formulation studies, ensuring the purity, quality, and efficacy of drugs while playing a crucial role from drug discovery to manufacturing.</p> Signup and view all the answers

<p>Fermentation, extraction, and purification</p> Signup and view all the answers