30 Questions
What is the primary difference between liquid chromatography and gas chromatography?
The stationary phase is contained in a column in gas chromatography, while it can be in a column or on a plane in liquid chromatography.
Which of the following is NOT a factor to consider when choosing an analytical method?
The color of the sample
What is the most popular category of columnar liquid chromatography for chemical analysis?
High-performance liquid chromatography (HPLC)
Which of the following is NOT listed as a potential application of chemical analysis?
Material science research
What is the main purpose of a mobile phase in chromatography?
To carry the sample through the stationary phase
Which of the following is the most important factor to consider when choosing an analytical method?
The required precision and accuracy of the assays
Which of the following is NOT a signal source discussed in the text?
Laser
What is the purpose of the flame in flame atomic emission spectroscopy?
To dissolve and evaporate the sample, creating free atoms for spectroscopic examination
Which statement about plasma is NOT true according to the text?
Plasma has a lower atomization efficiency compared to a flame
Which type of plasma is described as the most important in the text?
Inductively coupled plasma (ICP)
What is the purpose of the burner head in flame atomic emission spectroscopy?
To introduce the sample into the flame
How are arc and spark sources generated according to the text?
By flowing between two electrodes
What type of plasma is used in Direct Current Plasma (DCP) atomic emission spectroscopy?
Direct current plasma
Which part of the DCP instrument acts as a sample cell?
Cup-shaped graphite electrode
What is the purpose of nebulization in DCP atomic emission spectroscopy?
To convert the liquid sample into an aerosol
Which of the following is NOT a type of sample preparation method mentioned in the text?
Nebulization
What is the purpose of a monochromator in DCP atomic emission spectroscopy?
To choose the specific radiation emitted by the analyte and remove unwanted radiations
Which of the following nebulizers is NOT mentioned in the text?
Electrostatic nebulizer
What is the process that occurs simultaneously during a fluorescence measurement?
Activation and deactivation
What is the relationship used to calculate the concentration from the intensity of fluorescence?
Beer-Lambert law
Which type of fluorescence transition is most intense and useful for analytical determinations?
Resonance fluorescence
In resonance fluorescence, how does the wavelength of emitted radiation compare to the absorbed radiation?
The wavelength of emitted radiation is equal to the wavelength of absorbed radiation
What happens when a sample is introduced into a flame or other radiation source?
It absorbs photons and gets excited to a higher energy level
What happens to atoms in an excited state?
They return to the ground state by emitting a photon
What is the principle shared by all spectroscopic techniques?
To shine a beam of electromagnetic radiation onto a sample and observe how it responds
When did the history of spectroscopy begin?
In 1666-1672
What was the purpose of Joseph von Fraunhofer's experimental advances with dispersive spectrometers?
To make spectroscopy a more precise and quantitative scientific technique
What is the purpose of using spectroscopy as a tool?
To study the structures of atoms and molecules
What did Isaac Newton apply the word to describe?
The rainbow of colors that combine to form white light
What is the electromagnetic spectrum?
The range of frequencies of electromagnetic radiation and their respective wavelengths and photon energies
Test your knowledge of chromatography techniques such as liquid chromatography and gas chromatography. Learn about the stationary and mobile phases, columns used, and the differences between gas and liquid chromatography.
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