Chromatography Techniques Quiz

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Questions and Answers

What is the primary difference between liquid chromatography and gas chromatography?

  • Gas chromatography is more expensive than liquid chromatography.
  • Liquid chromatography is used for chemical analysis, while gas chromatography is used for environmental testing.
  • The stationary phase is contained in a column in gas chromatography, while it can be in a column or on a plane in liquid chromatography. (correct)
  • Gas chromatography is more precise and accurate than liquid chromatography.

Which of the following is NOT a factor to consider when choosing an analytical method?

  • The expected concentration range of the sample
  • The cost of the assay and the entire analysis
  • The color of the sample (correct)
  • The type of readily available instrument

What is the most popular category of columnar liquid chromatography for chemical analysis?

  • Ion exchange chromatography
  • High-performance liquid chromatography (HPLC) (correct)
  • Thin-layer chromatography
  • Size exclusion chromatography

Which of the following is NOT listed as a potential application of chemical analysis?

<p>Material science research (D)</p> Signup and view all the answers

What is the main purpose of a mobile phase in chromatography?

<p>To carry the sample through the stationary phase (B)</p> Signup and view all the answers

Which of the following is the most important factor to consider when choosing an analytical method?

<p>The required precision and accuracy of the assays (D)</p> Signup and view all the answers

Which of the following is NOT a signal source discussed in the text?

<p>Laser (A)</p> Signup and view all the answers

What is the purpose of the flame in flame atomic emission spectroscopy?

<p>To dissolve and evaporate the sample, creating free atoms for spectroscopic examination (C)</p> Signup and view all the answers

Which statement about plasma is NOT true according to the text?

<p>Plasma has a lower atomization efficiency compared to a flame (D)</p> Signup and view all the answers

Which type of plasma is described as the most important in the text?

<p>Inductively coupled plasma (ICP) (A)</p> Signup and view all the answers

What is the purpose of the burner head in flame atomic emission spectroscopy?

<p>To introduce the sample into the flame (B)</p> Signup and view all the answers

How are arc and spark sources generated according to the text?

<p>By flowing between two electrodes (B)</p> Signup and view all the answers

What type of plasma is used in Direct Current Plasma (DCP) atomic emission spectroscopy?

<p>Direct current plasma (A)</p> Signup and view all the answers

Which part of the DCP instrument acts as a sample cell?

<p>Cup-shaped graphite electrode (C)</p> Signup and view all the answers

What is the purpose of nebulization in DCP atomic emission spectroscopy?

<p>To convert the liquid sample into an aerosol (C)</p> Signup and view all the answers

Which of the following is NOT a type of sample preparation method mentioned in the text?

<p>Nebulization (C)</p> Signup and view all the answers

What is the purpose of a monochromator in DCP atomic emission spectroscopy?

<p>To choose the specific radiation emitted by the analyte and remove unwanted radiations (A)</p> Signup and view all the answers

Which of the following nebulizers is NOT mentioned in the text?

<p>Electrostatic nebulizer (B)</p> Signup and view all the answers

What is the process that occurs simultaneously during a fluorescence measurement?

<p>Activation and deactivation (D)</p> Signup and view all the answers

What is the relationship used to calculate the concentration from the intensity of fluorescence?

<p>Beer-Lambert law (B)</p> Signup and view all the answers

Which type of fluorescence transition is most intense and useful for analytical determinations?

<p>Resonance fluorescence (C)</p> Signup and view all the answers

In resonance fluorescence, how does the wavelength of emitted radiation compare to the absorbed radiation?

<p>The wavelength of emitted radiation is equal to the wavelength of absorbed radiation (D)</p> Signup and view all the answers

What happens when a sample is introduced into a flame or other radiation source?

<p>It absorbs photons and gets excited to a higher energy level (C)</p> Signup and view all the answers

What happens to atoms in an excited state?

<p>They return to the ground state by emitting a photon (D)</p> Signup and view all the answers

What is the principle shared by all spectroscopic techniques?

<p>To shine a beam of electromagnetic radiation onto a sample and observe how it responds (D)</p> Signup and view all the answers

When did the history of spectroscopy begin?

<p>In 1666-1672 (A)</p> Signup and view all the answers

What was the purpose of Joseph von Fraunhofer's experimental advances with dispersive spectrometers?

<p>To make spectroscopy a more precise and quantitative scientific technique (B)</p> Signup and view all the answers

What is the purpose of using spectroscopy as a tool?

<p>To study the structures of atoms and molecules (B)</p> Signup and view all the answers

What did Isaac Newton apply the word to describe?

<p>The rainbow of colors that combine to form white light (B)</p> Signup and view all the answers

What is the electromagnetic spectrum?

<p>The range of frequencies of electromagnetic radiation and their respective wavelengths and photon energies (D)</p> Signup and view all the answers

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