Podcast
Questions and Answers
Which of the following is NOT a function of intracellular protein degradation?
Which of the following is NOT a function of intracellular protein degradation?
- Preventing the accumulation of abnormal proteins
- Facilitating the synthesis of abnormal proteins (correct)
- Regulating the turnover of proteins
- Providing the cell with a constant supply of amino acids
What is the approximate half-life of 'normally' proteins?
What is the approximate half-life of 'normally' proteins?
- 200-300 hours
- 10-20 hours
- 100-200 hours (correct)
- 1-2 days
Which type of proteins have a shorter half-life?
Which type of proteins have a shorter half-life?
- Transcription factors
- Special cases proteins
- Enzymes that catalyze committed steps (correct)
- Structural proteins
In which compartment does lysosomal protein degradation occur?
In which compartment does lysosomal protein degradation occur?
What is the pH of lysosomes where protein degradation occurs?
What is the pH of lysosomes where protein degradation occurs?
Which type of proteins are primarily degraded by the cytosol-ubiquitin dependent proteasome system?
Which type of proteins are primarily degraded by the cytosol-ubiquitin dependent proteasome system?
What is the half-life of Acetyl CoA carboxylase in a fasted state?
What is the half-life of Acetyl CoA carboxylase in a fasted state?
Which tissue has the shortest half-life for Lactic Acid Dehydrogenase?
Which tissue has the shortest half-life for Lactic Acid Dehydrogenase?
Which organelles contain digestive enzymes such as lipases, nucleases, and proteases?
Which organelles contain digestive enzymes such as lipases, nucleases, and proteases?
What percentage of protein degradation occurs in the acidified compartment of lysosomes?
What percentage of protein degradation occurs in the acidified compartment of lysosomes?
What is the main process by which vesicles are formed to engulf small amounts of cytoplasm or specific organelles?
What is the main process by which vesicles are formed to engulf small amounts of cytoplasm or specific organelles?
Which nutritional state results in a longer half-life for Acetyl CoA carboxylase?
Which nutritional state results in a longer half-life for Acetyl CoA carboxylase?
What is responsible for the breakdown of most short-lived proteins in human cells?
What is responsible for the breakdown of most short-lived proteins in human cells?
Which protein is tagged by the covalent attachment of ubiquitin for degradation?
Which protein is tagged by the covalent attachment of ubiquitin for degradation?
What is the function of the 19S regulatory particle in the 26S proteasome?
What is the function of the 19S regulatory particle in the 26S proteasome?
What is responsible for the catalysis of the transfer of ubiquitin from E2 to a lysine amino group of the condemned protein?
What is responsible for the catalysis of the transfer of ubiquitin from E2 to a lysine amino group of the condemned protein?
What is recycled in the process of proteasomal degradation?
What is recycled in the process of proteasomal degradation?
Which component is important for the translocation of unfolded proteins into the central core of the proteasome?
Which component is important for the translocation of unfolded proteins into the central core of the proteasome?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
What is the function of Deubiquitinating enzymes (DUBs)?
What is the function of Deubiquitinating enzymes (DUBs)?
Which enzyme family contains relatively small proteins (< 40-kDa)?
Which enzyme family contains relatively small proteins (< 40-kDa)?
What is the role of UBP (UBiquitin-specific Processing proteases) gene family?
What is the role of UBP (UBiquitin-specific Processing proteases) gene family?
What is structurally formed from 7 alpha subunits and 7 beta subunits?
What is structurally formed from 7 alpha subunits and 7 beta subunits?
What is involved in regulation of DNA repair, transcription, and endocytosis?
What is involved in regulation of DNA repair, transcription, and endocytosis?
Which organelles contain digestive enzymes such as lipases, nucleases, and proteases?
Which organelles contain digestive enzymes such as lipases, nucleases, and proteases?
What is the pH of lysosomes where protein degradation occurs?
What is the pH of lysosomes where protein degradation occurs?
What is the main process by which vesicles are formed to engulf small amounts of cytoplasm or specific organelles?
What is the main process by which vesicles are formed to engulf small amounts of cytoplasm or specific organelles?
Which nutritional state results in a longer half-life for Acetyl CoA carboxylase?
Which nutritional state results in a longer half-life for Acetyl CoA carboxylase?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
Which component is responsible for the recognition and binding of polyubiquitinated proteins, removal of ubiquitin, unfolding the protein substrate, and translocation into the central core of the proteasome?
Which component is responsible for the recognition and binding of polyubiquitinated proteins, removal of ubiquitin, unfolding the protein substrate, and translocation into the central core of the proteasome?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
What is the critical number of ubiquitin molecules on a target protein for efficient degradation?
What is the function of Deubiquitinating enzymes (DUBs)?
What is the function of Deubiquitinating enzymes (DUBs)?
What is the role of UBP (UBiquitin-specific Processing proteases) gene family?
What is the role of UBP (UBiquitin-specific Processing proteases) gene family?
What is responsible for the breakdown of most short-lived proteins in human cells?
What is responsible for the breakdown of most short-lived proteins in human cells?
Explain the process of protein degradation through ubiquitination and proteasomal degradation. What are the key enzymes and components involved in this process?
Explain the process of protein degradation through ubiquitination and proteasomal degradation. What are the key enzymes and components involved in this process?
What is the role of deubiquitinating enzymes (DUBs) in the protein degradation process?
What is the role of deubiquitinating enzymes (DUBs) in the protein degradation process?
Describe the structural composition of the 26S proteasome. What are the functions of its core and regulatory particle?
Describe the structural composition of the 26S proteasome. What are the functions of its core and regulatory particle?
How is ubiquitin recycled in the process of proteasomal degradation?
How is ubiquitin recycled in the process of proteasomal degradation?
What is the minimum number of ubiquitin molecules required on a target protein for efficient degradation?
What is the minimum number of ubiquitin molecules required on a target protein for efficient degradation?
