Infectious Diseases and Diagnostics Quiz
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Questions and Answers

What is the primary cause of Enteric Fever?

  • Escherichia coli
  • Streptococcus pyogenes
  • Salmonella Typhi (correct)
  • Rickettsia rickettsii
  • What is a characteristic symptom of Typhoid Fever?

  • Macular rash
  • Erysipelas
  • Elevated zinc concentration
  • Severe headache (correct)
  • Which of the following best describes the bacterial attachment mechanism in enteric fever?

  • Cholesterol-rich membranes
  • Sialic acid absence
  • Vi Capsule (correct)
  • Flagella movement
  • Which laboratory findings are commonly associated with acute Typhoid Fever?

    <p>Decreased platelets and anemia (D)</p> Signup and view all the answers

    What challenge does Salmonella Typhi face upon initial infection?

    <p>Low initial immunological response (C)</p> Signup and view all the answers

    What is the first step in the process of infection?

    <p>Entry and attachment to the body (A)</p> Signup and view all the answers

    Which process allows pathogens to evade the host's innate immune response?

    <p>Colonisation and spread (B)</p> Signup and view all the answers

    How might the immune response contribute to disease pathology?

    <p>Through bystander damage to host tissues (A)</p> Signup and view all the answers

    What is a consequence of the local or general spread of infection within the body?

    <p>Access to necessary nutrients (C)</p> Signup and view all the answers

    Which of the following best describes the exit stage of infection?

    <p>Pathogens leave the body for transmission (C)</p> Signup and view all the answers

    Which organism is detected using anti-Leishmania antibodies in diagnosis?

    <p>Cutaneous Leishmaniasis (A)</p> Signup and view all the answers

    Which microscopy method is used for identifying simian vacuolating virus 40 in brain tissue?

    <p>In situ hybridization (A)</p> Signup and view all the answers

    What is a key limitation of diagnostic histopathology in emerging infectious diseases?

    <p>Availability of specific antibodies (A)</p> Signup and view all the answers

    Which infection is confirmed using a PCR assay according to the content?

    <p>Tularemia (C)</p> Signup and view all the answers

    Which organism is associated with granulomatous inflammation in lung infections?

    <p>Tularemia (B)</p> Signup and view all the answers

    What technique is primarily used for studying the interaction between Bacillus anthracis and endothelial cells?

    <p>Electron microscopy (D)</p> Signup and view all the answers

    Which of the following diseases is NOT categorized as emerging infectious diseases?

    <p>Syphilis (C)</p> Signup and view all the answers

    Which diagnostic method is recommended for detecting Cryptococcus in tissue samples?

    <p>Immunohistochemistry (A)</p> Signup and view all the answers

    What is the primary focus of epidemiology in infectious diseases?

    <p>Controlling and reducing disease incidence (D)</p> Signup and view all the answers

    Which branch of infectious disease epidemiology focuses on the cause of diseases?

    <p>Disease aetiology (C)</p> Signup and view all the answers

    What is one effective method for reducing the spread of infectious diseases?

    <p>Improving personal hygiene and sanitation (B)</p> Signup and view all the answers

    Which method is commonly used to protect against disease vectors like mosquitoes?

    <p>Physical barriers and chemical treatments (C)</p> Signup and view all the answers

    What does the term 'quarantine' refer to in the context of infectious diseases?

    <p>Physical separation to prevent transmission (B)</p> Signup and view all the answers

    Which of the following is a method used in the diagnosis of infectious diseases?

    <p>Immunohistochemistry (A)</p> Signup and view all the answers

    In what way can microscopy be utilized in diagnosing infectious diseases?

    <p>Staining for microorganisms (B)</p> Signup and view all the answers

    Which inflammatory reaction type is associated with a specific microbe?

    <p>Granulomatous (B)</p> Signup and view all the answers

    What are the key characteristics of acute infectious diseases?

    <p>They progress quickly. (D)</p> Signup and view all the answers

    Which of the following accurately describes chronic infectious diseases?

    <p>They progress slowly and can be long lasting. (D)</p> Signup and view all the answers

    How is a latent infectious disease characterized?

    <p>There are no symptoms between outbreaks. (B)</p> Signup and view all the answers

    What distinguishes systemic infectious diseases from local infections?

    <p>They infect multiple body sites and tissues. (B)</p> Signup and view all the answers

    Which of the following conditions is classified as a local infectious disease?

    <p>Ringworm (tinea) (C)</p> Signup and view all the answers

    What is a common symptom associated with the early stages of Ebola?

    <p>Fever and fatigue (B)</p> Signup and view all the answers

    Which virus is associated with the transformation of cells into malignant carcinoma?

    <p>Human Papilloma Virus (HPV) (B)</p> Signup and view all the answers

    What is the typical onset period for ringworm infection?

    <p>4-14 days (D)</p> Signup and view all the answers

    Which statement is true regarding the spread of the herpes simplex virus (HSV)?

    <p>It can spread from oral to genital areas. (B)</p> Signup and view all the answers

    What type of infectious disease is characterized by a state of quiescence in the neuron nucleus?

    <p>Latent disease (D)</p> Signup and view all the answers

    What was the main focus of the Human Microbiome Project?

    <p>To analyze the composition and functions of human-associated microorganisms (C)</p> Signup and view all the answers

    Which of the following conditions has been linked to Helicobacter pylori infection?

    <p>Gastric ulcer (A)</p> Signup and view all the answers

    What is the significance of the Henle-Koch postulates in the study of infectious diseases?

    <p>They establish criteria for identifying the causative agent of a disease. (D)</p> Signup and view all the answers

    Which population aspect is analyzed in the study of cervical cancer elimination targets by Canfell et al.?

    <p>Comparative modeling in low-income and lower-middle-income countries (A)</p> Signup and view all the answers

    What is a consequence of untreated Helicobacter pylori infection as suggested by Mohamed et al.?

    <p>Increased risk of liver disease (A)</p> Signup and view all the answers

    What is primarily being evaluated in the research by Bowen et al. on HIV-associated infections?

    <p>The pathophysiology and treatment of CNS infections (C)</p> Signup and view all the answers

    What is the goal of personalized treatment benefit index in antiretroviral therapy as mentioned by Xiao et al.?

    <p>To tailor treatment recommendations based on individual life expectancy (D)</p> Signup and view all the answers

    What kind of modeling analysis is applied to assess the impact of cervical cancer elimination targets?

    <p>Comparative modeling analysis (A)</p> Signup and view all the answers

    Flashcards

    Entry and Attachment

    How a pathogen enters the body, attaches, and overcomes the initial defenses.

    Colonisation and Spread

    The pathogen establishes itself in the body, possibly spreading to new areas.

    Replication

    The pathogen makes copies of itself within the host's cells.

    Exit (Shedding)

    The pathogen leaves the infected host, allowing it to spread to others.

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    Pathology and Damage

    The pathogen causes damage to the host, either directly or indirectly through the host's immune response.

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    Acute Infectious Disease

    A disease that develops quickly with intense symptoms, lasting only a short time.

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    Chronic Infectious Disease

    A disease that develops more slowly, with less severe symptoms, but lasts for a longer period.

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    Latent Infectious Disease

    A disease that has periods without symptoms, but can reactivate and cause illness.

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    Local Infection

    Refers to an infection that is confined to a specific area of the body.

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    Systemic Infection

    Refers to an infection that spreads throughout the body, affecting multiple tissues and organs.

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    Duration of a Disease

    The duration of a disease refers to how long the symptoms last.

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    Location of a Disease

    The location of a disease refers to where in the body the infection is taking place.

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    Ringworm

    A fungal infection of the skin, also known as 'ringworm'.

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    Human Papillomavirus (HPV)

    A virus known to cause various cancers, including cervical cancer.

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    Ebola Virus

    A virus that causes a hemorrhagic fever, characterized by severe bleeding and organ failure.

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    Salmonella Typhi

    A gram-negative bacterium that infects humans, causing typhoid fever.

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    Vi Capsule

    A protein capsule found on the surface of Salmonella Typhi that helps the bacteria attach to human cells.

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    Carrier State

    A state where an individual carries Salmonella Typhi without exhibiting symptoms but can still transmit the bacterium to others.

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    Cellular Response

    A type of immune response characterized by the activation of white blood cells to fight off infection.

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    α1-antitrypsin

    A protein found in the liver that increases during liver damage or inflammation, which can indicate complications associated with typhoid fever.

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    Infectious Disease Epidemiology

    A scientific field that investigates patterns, causes, and effects of diseases on populations, focusing on controlling and reducing disease incidence by identifying factors that contribute to disease causation, transmission, and prevention.

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    Disease Aetiology

    The study of the causative agents of a disease. This area of epidemiology explores the factors contributing to the origin and development of infectious diseases.

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    Outbreak Investigation

    A focused investigation into a disease outbreak, aiming to trace its source, identify contributing factors, and develop strategies for containment.

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    Disease Screening & Surveillance

    Systematic procedures used to detect and monitor diseases within populations. They include screenings to identify early cases and ongoing surveillance to track disease trends.

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    Treatment or Effect Comparisons (Clinical Trials)

    Methods used to compare the effectiveness of different treatments or interventions for a particular disease. This includes clinical trials to evaluate treatment options and their impact on patient outcomes.

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    Quarantine

    Measures that physically separate infected individuals from healthy individuals to prevent further transmission of the disease. This is crucial for containing highly contagious or severe infections.

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    Detection of Microorganism

    Methods used to detect the presence of a specific microorganism in a sample. This can involve culturing the organism, using specific antibodies, or analyzing genetic material.

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    Pathology

    The microscopic examination of tissues to identify the presence of infectious agents, understand their location and characteristics, and determine the type of inflammatory response the host is exhibiting.

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    Etiology

    The study of the causes and origins of diseases, focusing on how factors contribute to their development.

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    Koch's Postulates

    A set of criteria used to establish a causal relationship between a microbe and a disease. It involves demonstrating the microbe's presence in diseased individuals, isolating and cultivating the microbe, introducing the microbe to healthy individuals and observing the disease, and re-isolating the microbe from the newly infected individuals.

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    Human Microbiome

    The interaction between a host and its microbial community, including bacteria, viruses, fungi, and others.

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    Helicobacter pylori

    A type of bacterial infection that affects the stomach and intestines, often leading to ulcers and gastritis.

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    Ziehl-Neelson Stain

    A stain used to identify mycobacteria (e.g., Mycobacterium leprae) in tissue samples. It uses a combination of carbol fuchsin dye for staining and acid alcohol for decolorization, leaving the mycobacteria stained pink against a blue background.

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    Histoplasmosis

    A fungal disease caused by the fungus Histoplasma capsulatum. The fungus is often found in bird and bat droppings, and infection can occur through inhalation of the fungus.

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    Gomori-Grocott Stain

    A stain used to visualize fungal organisms in tissue samples, such as Histoplasma capsulatum. It employs a silver stain that highlights the fungal cell walls, making them appear black against a light background.

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    Cryptococcosis

    A fungal disease caused by the Cryptococcus neoformans yeast. The fungus can infect the lungs, brain, and other organs.

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    India Ink Stain

    A blue stain used to visualize the capsule surrounding Cryptococcus neoformans yeast in tissue samples. It stains the capsule blue against a light background, highlighting its presence.

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    Syphilis

    A bacterial infection caused by the Treponema pallidum spirochete. Syphilis is known for its diverse and often complex stages of infection, affecting various organs and tissues.

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    Warthin-Starry Stain

    A stain used to visualize Treponema pallidum spirochetes in tissue samples under a microscope. It uses silver salts to stain the organisms' structure.

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    In Situ Hybridization (ISH)

    A technique used to detect specific genetic sequences within tissue samples. It's a valuable tool for identifying and characterizing specific microorganisms, including viruses, bacteria, and fungi.

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    Study Notes

    Integrated Pathology: Infectious Disease

    • Infectious disease is detected when pathological effects occur in tissues/cells caused by a contagious agent that transmits infection.
    • This has implications for diagnosis, treatment, prognosis, and disease prevention.
    • Infectious diseases have a causative agent (aetiological agent) typically microscopic (microorganism/microbes).
    • Substances containing the infectious microorganism are called etiological agents.
    • An infection is damage to tissues/cells leading to symptomatic disease.

    Learning Outcomes

    • LO1: Understand how infectious diseases are characterized in relation to disease stages.
    • LO2: Explain the causes of pathology due to infectious diseases.
    • LO3: Discuss how infectious diseases cause varied pathologies with named examples.

    Pathology and Infectious Diseases

    • Infectious diseases are caused by pathogens;
    • Diagnosis, treatment, prognosis, and prevention strategies vary based on the causative agent.
    • Knowing the causative agent is essential for all these aspects.

    Pathological Effects of Infectious Diseases

    • Infectious diseases are easily transmitted, especially through air or water.
    • The causative agent typically consists of microorganisms or microbes.

    Causes of Infectious Disease

    • Infectious diseases are caused by a variety of agents:
      • Bacteria (prokaryotes)
      • Viruses (non-cellular)
      • Fungi (eukaryotes)
      • Protozoa (eukaryotes)
      • Prions (proteins, non-cellular)
      • Helminths (non-microbial, eukaryotes)
      • Arthropods (non-microbial, eukaryotes)
      • Archaea (have no infectious agent identified thus far but may have a role in periodontal disease)
    • Each of these agents has unique characteristics (e.g., organism type, replication process, structure, composition, and genetics) influencing how they interact with the host and cause disease.

    When Infectious Diseases Occur

    • The human microbiome includes Archaea, bacteria, fungi, and viruses.

    • Commensal organisms are part of the normal microbiome.

    • Obligate pathogens always cause disease.

    • Opportunistic pathogens cause disease under specific conditions.

    Koch's Postulates (1890)

    • The causative agent must be present in all cases of the disease and not present in healthy individuals.
    • The causative agent must be isolated in pure culture.
    • The agent from pure culture must cause the disease in a model system.
    • The agent must be re-isolated from the diseased model.
    • Establishing causation cannot be based solely on association.

    Routes of Transmission

    • Aerosol (inhalation)
    • Oral (ingestion of contaminated food/water)
    • Direct contact (contact with infected individual tissues/bodily fluids)
    • Fomite transmission (contaminated surfaces of inanimate objects)
    • Vector-borne (other living organisms)
    • Zoonotic (originating from other animals)

    Transmission of Disease-Causing Agents

    • Infectious agents must be transmitted between hosts.
    • Transmission routes depend on the microorganism and the type of tissue/cell affected.
      • Example: Rhinoviruses (common cold) infect the respiratory tract, spreading through droplets from coughing/sneezing.

    Case Study: Bacillus anthracis

    • Gram-positive bacilli, endospore-forming, found in soil, water, and contaminated animal products (e.g., wool, hides, hair).
    • Occurs globally.
    • Has a capsule and two toxins (lethal factor and edema factor).
    • Types of anthrax:
      • Cutaneous
      • Gastrointestinal
      • Inhalation
      • Injection
      • Welder's
    • Transmission route determines the type of anthrax.

    Case Study: Pathologies and Transmission: Bacillus anthracis

    • Different transmission routes result in distinct pathologies:
      • Cutaneous anthrax: wound contamination; ~95% cases
      • Gastrointestinal anthrax: Spores from infected meat; >50% fatality if untreated.
      • Inhalation anthrax: Breathing contaminated spores; 100% mortality if untreated
    • Injection anthrax: Needles with contaminated soil/material.
    • Welder's anthrax: similar to infection, with pneumonia as diagnosis

    Pathogenesis of Infectious Diseases (General)

    • Agents cause disease through various mechanisms, including:
      • Toxin production (destroying cells/tissues)
      • Direct invasion/destruction of host cells
      • Triggering immune responses in the host (e.g., fever, sneezing, vomiting).

    Steps in Infection

    • Entry and attachment to the body
    • Colonization (local/general spread)
    • Replication
    • Exit (shedding from the body)
    • The pathology of infectious diseases results from these steps.

    Pathogenesis of Infectious Diseases (Skin and Soft Tissue)

    • Mucocutaneous lesions
    • Abscess formation
    • Spreading infections
    • Necrotizing infections
    • Skin manifestations from systemic infections
    • Different skin structures can be affected in various ways; i.e. epidermis, dermis, hair follicles.

    Insight to Site-Specific Pathogenesis (Skin Infections)

    • Superficial to invasive skin infections have different causative agents and site of infection.

    Classification of Infectious Diseases

    • By location: Local (confined to an area of the body), Systemic (multiple body sites/tissues)

    • By timing: Primary (initial infection in a healthy person), Secondary (infection in a weakened person).

    Case Study: Influenza Syndrome

    • Causative agent: Influenza virus (segmented negative-sense RNA genome).
    • Symptoms: fever, cough, sore throat, congestion, muscle/body aches, fatigue, vomiting, diarrhea.

    Clinical Signs and Symptoms (Covid-19, Flu & Cold)

    • A table comparing symptom frequency in Covid-19, Flu, and Cold. (using a chart with different icons for each disease)

    Characteristics of Infectious Diseases (By Duration)

    • Acute: Develops and progresses quickly
    • Chronic: Develops slowly, less severe, but long-lasting
    • Latent: No symptoms between outbreak and illness

    Case Study: Enteric Fever (S. Typhi)

    • Gram-negative bacteria
    • Human-specific—high fever, fatigue, headache, nausea, abdominal pain, and constipation or diarrhea
    • Host tropism—Vi capsule attaches to human cells.
    • Primary infection; secondary infection; carrier state
    • Low initial immunological response, regulation of Vi capsule

    Reducing the Spread of Infectious Diseases

    • Removing the infectious agent (e.g., good hygiene/sanitation, handwashing, vector control).
    • Prevention against vectors.
    • Quarantine of infected individuals.

    Diagnosis of Infectious Diseases

    • Clinical presentation and symptoms
    • Detection methods include signs & symptoms, detection of microorganism, pathology (diagnostic histopathology, molecular pathology, immunohistochemistry, in situ hybridization).
    • Determining the type of inflammatory response.

    Diagnosis using Microscopy

    • Staining methods—staining microbes for visualization (A-Leprosy, B-Histoplasmosis, C-Cryptococcus, D-Syphilis).
    • Immunohistochemistry: tissue staining with antibodies for various microbes.
    • Other methods—use of in situ hybridization with electron microscopy, molecular biology (PCR)-infection localization, types of inflammatory reaction.

    Diagnosis of Infectious Diseases (Emerging)

    • Diagnostic histopathology is useful in emerging infections, like Zika, HIV, and others.
    • Limitations include antibody availability and the potential for genomics replacement.

    Emerging Infectious Diseases

    • Recent diseases with increasing incidence.
    • Examples: Gonorrhea (antibiotic resistance), Measles (vaccine inefficacy), and Zika virus

    Use of Infectious Disease Epidemiology

    • Epidemiology is the study of disease patterns, helping to understand and control infectious diseases.
    • Epidemiologists use several methods: Disease etiology, outbreak investigation, screening and surveillance, and comparisons of treatments.

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    Description

    Test your knowledge on infectious diseases, specifically focusing on enteric fever and typhoid fever. This quiz covers key symptoms, mechanisms of infection, laboratory findings, and diagnostic methods related to various pathogens. Perfect for students studying microbiology and infectious disease pathology.

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