Immunology: Ag-Ab Reactions and Blood Components

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What is the principle behind the Complement Fixation Test?

Toxins are labeled with detector molecules
Antibodies neutralize viruses
Antigens bond with enzymes
Complement proteins bind to Ag-Ab complex (correct)

What is the Wasserman test used to diagnose?

Diphtheria
Toxoplasmosis
Syphilis (correct)
Rabies

What is the purpose of Neutralization Antibodies in serum?

To detect tumor markers
To diagnose rabies
To track disease outbreaks
To neutralize Ag on the surface of viruses (correct)

What is the principle behind ELISA?

Enzyme linked Ig detects Ag/Ab (B) Signup and view all the answers

What is the main advantage of Competitive ELISA?

Less sample purification (B) Signup and view all the answers

What is the Sandwich ELISA used for?

To screen Ag (A) Signup and view all the answers

What is the term for the fluid portion of blood with no clotting factors?

Serum (D) Signup and view all the answers

Which type of Ag-Ab reaction involves the formation of an insoluble precipitate band?

Precipitation reaction (C) Signup and view all the answers

What is the term for the test where Ag is layered over antiserum in a test tube?

Ring test (A) Signup and view all the answers

Which procedure involves the formation of a precipitin band in a single diffusion in one dimension?

Oudin procedure (A) Signup and view all the answers

What is the term for the test where a drop of Ag is added to a drop of patient's serum on a cavity slide?

Flocculation test (D) Signup and view all the answers

Which type of electroimmunodiffusion test is used for the quantitative estimation of Ag?

Rocket electrophoresis (B) Signup and view all the answers

What is the main difference between lateral flow assay and western blot?

Lateral flow assay is a rapid test, while western blot is labor intensive (A) Signup and view all the answers

What is the purpose of the control line in lateral flow assay?

To verify the binding of Ab specific to the labeled Ab (B) Signup and view all the answers

Which technique is commonly used for the detection of infectious diseases?

Western blot (A) Signup and view all the answers

What is the main advantage of flow through assay?

It has good sensitivity to Ab (B) Signup and view all the answers

What is the main limitation of lateral flow assay?

It has low sensitivity (B) Signup and view all the answers

What is the primary function of ELFA in biological samples?

To detect and quantify proteins (D) Signup and view all the answers

What is the main difference between flow through assay and western blot?

Flow through assay is a semi-dry dot-blot, while western blot is an electrophoresis technique (B) Signup and view all the answers

Which type of immunofluorescence assay uses two antibodies, one labeled with a fluorophore?

Indirect Immunofluorescence (B) Signup and view all the answers

What is the purpose of radioactively labeled antibodies or antigens in Radioimmunoassay?

To compete with patient's unlabeled antibodies or antigens for binding sites (A) Signup and view all the answers

Which of the following is a limitation of Radioimmunoassay?

Limited use due to radioisotopes (C) Signup and view all the answers

What is the function of the spectroflurometer in ELFA?

To measure the fluorescent product (D) Signup and view all the answers

Which of the following assays is known for its high sensitivity and specificity?

Immunoradiometric assay (C) Signup and view all the answers

What is a characteristic of Rapid Diagnostic Tests?

They are simple to perform with a low risk of incorrect results (B) Signup and view all the answers

What type of analyte is detected in a Dengue rapid test?

Antigen (B) Signup and view all the answers

Which of the following is an example of a multiplex molecular panel?

CNS, GI, and RT infections panel (C) Signup and view all the answers

What is the purpose of incorporating molecular methods in scientific research?

To detect and study infectious pathogens (D) Signup and view all the answers

What is an example of a nucleic acid amplification test?

RT-PCR for SARS-CoV2 (B) Signup and view all the answers

What is the advantage of molecular methods in diagnostics?

They provide short performance times for clinical decisions (B) Signup and view all the answers