30 Questions
What is the principle behind the Complement Fixation Test?
Complement proteins bind to Ag-Ab complex
What is the Wasserman test used to diagnose?
Syphilis
What is the purpose of Neutralization Antibodies in serum?
To neutralize Ag on the surface of viruses
What is the principle behind ELISA?
Enzyme linked Ig detects Ag/Ab
What is the main advantage of Competitive ELISA?
Less sample purification
What is the Sandwich ELISA used for?
To screen Ag
What is the term for the fluid portion of blood with no clotting factors?
Serum
Which type of Ag-Ab reaction involves the formation of an insoluble precipitate band?
Precipitation reaction
What is the term for the test where Ag is layered over antiserum in a test tube?
Ring test
Which procedure involves the formation of a precipitin band in a single diffusion in one dimension?
Oudin procedure
What is the term for the test where a drop of Ag is added to a drop of patient's serum on a cavity slide?
Flocculation test
Which type of electroimmunodiffusion test is used for the quantitative estimation of Ag?
Rocket electrophoresis
What is the main difference between lateral flow assay and western blot?
Lateral flow assay is a rapid test, while western blot is labor intensive
What is the purpose of the control line in lateral flow assay?
To verify the binding of Ab specific to the labeled Ab
Which technique is commonly used for the detection of infectious diseases?
Western blot
What is the main advantage of flow through assay?
It has good sensitivity to Ab
What is the main limitation of lateral flow assay?
It has low sensitivity
What is the primary function of ELFA in biological samples?
To detect and quantify proteins
What is the main difference between flow through assay and western blot?
Flow through assay is a semi-dry dot-blot, while western blot is an electrophoresis technique
Which type of immunofluorescence assay uses two antibodies, one labeled with a fluorophore?
Indirect Immunofluorescence
What is the purpose of radioactively labeled antibodies or antigens in Radioimmunoassay?
To compete with patient's unlabeled antibodies or antigens for binding sites
Which of the following is a limitation of Radioimmunoassay?
Limited use due to radioisotopes
What is the function of the spectroflurometer in ELFA?
To measure the fluorescent product
Which of the following assays is known for its high sensitivity and specificity?
Immunoradiometric assay
What is a characteristic of Rapid Diagnostic Tests?
They are simple to perform with a low risk of incorrect results
What type of analyte is detected in a Dengue rapid test?
Antigen
Which of the following is an example of a multiplex molecular panel?
CNS, GI, and RT infections panel
What is the purpose of incorporating molecular methods in scientific research?
To detect and study infectious pathogens
What is an example of a nucleic acid amplification test?
RT-PCR for SARS-CoV2
What is the advantage of molecular methods in diagnostics?
They provide short performance times for clinical decisions
Learn about the different types of Ag-Ab reactions, including precipitation reactions, and the difference between serum and plasma in the blood. Understand the concepts of sensitivity and specificity in immunology.
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