Human Histology Unit 1 Quiz

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Questions and Answers

Within the context of the provided material, what is the primary focus of the 'Prayer before study'?

  • To request assistance for effective communication and clarity of thinking. (correct)
  • To express gratitude for past knowledge and academic achievements.
  • To seek divine guidance for physical health and well-being.
  • To invoke blessings for financial abundance during studies.

According to the provided document, what is the primary focus of the first unit of the Human Histology course?

  • Acquiring advanced knowledge of cellular pathology identification.
  • Hands-on experience with complex electron microscopy procedures.
  • Detailed examination of specialized staining techniques.
  • Understanding core principles and the significance of human histology in relation to the Medical Technology profession. (correct)

What is the listed learning objective regarding the end of the first unit?

  • Being able to perform advanced in-vivo imaging studies.
  • Developing the ability to lead a research project in medical microbiology.
  • Comprehending the application of histology within the Medical Technology field and the methodology in tissue preparation. (correct)
  • Mastering the art of tissue dissection with anatomical precision.

Which of the following items is NOT a listed course content topic?

<p>Advanced Molecular Biology Techniques (A)</p> Signup and view all the answers

What is the academic year and semester for the presented course?

<p>Second Semester A.Y. 2024-2025 (B)</p> Signup and view all the answers

What does the term 'histology' primarily refer to?

<p>The scientific study of microscopic structures of tissues and organs. (A)</p> Signup and view all the answers

In the context of the Philippines, what is the primary role of Registered Medical Technologists (RMTs) regarding histology?

<p>Assisting pathologists by preparing and processing tissue samples. (C)</p> Signup and view all the answers

What is the main difference between a histotechnician and a histotechnologist in the United States?

<p>Histotechnicians focus on routine techniques, while histotechnologists perform both routine and complex techniques. (D)</p> Signup and view all the answers

What is the primary purpose of the embedding step in tissue processing?

<p>To provide support to the tissue and permit thin sectioning. (A)</p> Signup and view all the answers

What is the primary goal of fixation in tissue processing?

<p>To preserve the tissue in a state as close to living as possible. (B)</p> Signup and view all the answers

Which of the following is a commonly used medium for embedding tissue?

<p>Paraffin wax (A)</p> Signup and view all the answers

Why is dehydration a crucial step in tissue processing?

<p>To remove water molecules from the tissue (A)</p> Signup and view all the answers

What instrument is used to cut tissue specimens into thin sections during the sectioning step?

<p>Microtome (B)</p> Signup and view all the answers

In tissue processing, what is the purpose of using a water bath?

<p>To flatten the tissue ribbon before mounting. (B)</p> Signup and view all the answers

Which of the following is the main purpose of clearing in tissue processing?

<p>To remove dehydrating agents and improve microscopic examination. (B)</p> Signup and view all the answers

What is the common fixative used in histology?

<p>Formalin (D)</p> Signup and view all the answers

What is Meyer's egg albumin commonly used for in the tissue processing procedure?

<p>As an adhesive to attach tissue to a slide. (C)</p> Signup and view all the answers

Which of these statements is true regarding the levels of organization in the human body, according to normal hierarchical order?

<p>Molecules combined to form cells, which form tissues and then organs before forming organ systems. (B)</p> Signup and view all the answers

Which of the following is commonly used as a stain in histology?

<p>Hematoxylin and Eosin (B)</p> Signup and view all the answers

What is the purpose of mounting a tissue specimen with Canada balsam and a coverslip?

<p>To secure the specimen and prevent damage. (C)</p> Signup and view all the answers

What are artifacts in the context of histology?

<p>Errors in the tissue preparation process. (D)</p> Signup and view all the answers

Flashcards

Human Histology

The study of the microscopic structure of human tissues.

Tissue Processing

Methods to prepare tissue samples for microscopic examination.

Histologic Slide Examination

Analyzing prepared tissue slides under a microscope.

Relevance to Medical Technology

The importance of histology in diagnosing and treating diseases.

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Learning Outcomes

Expected competencies after studying human histology.

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Histology

Scientific study of microscopic structures of tissues and organs.

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Histotechnician

Prepares and processes tissue samples for histologic techniques.

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Histotechnologist

Performs both routine and complex histologic techniques.

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Fixation

Process of preserving tissue structure by cross-linking proteins.

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Dehydration

Removal of water from tissue using increasing alcohol concentration.

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Clearing

Removes dehydrating agent and replaces it with an organic solvent.

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Infiltration

Filling tissue with a melted medium for embedding.

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Common Fixatives

Substances like formalin used to preserve tissues during fixation.

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Embedding

Infiltrated tissue is placed in a mold with media and allowed to harden.

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Trimming

The paraffin block is trimmed to expose the tissue for sectioning.

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Sectioning

Cuts tissue into thin ribbons for microscopy using a microtome.

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Water Bath

Tissue ribbons are floated to flatten, then placed on slides.

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Staining

Applying stains to visualize different tissue components.

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Mounting

Adding a mounting agent and coverslip to protect the specimen.

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Common Stain: Hematoxylin and Eosin

These stains are commonly used to visualize tissue architecture.

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Artifacts in Histology

Errors that occur in the preparation process of tissue samples.

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Study Notes

Prayer Before Study

  • A prayer for guidance and understanding during study sessions.
  • Acknowledges God as the source of light and wisdom.
  • Requests a keen understanding, good memory, and the capacity to grasp concepts correctly.
  • Asks for accuracy and clarity in expressing ideas.
  • Seeks God's presence at the start, guidance throughout, and completion of the work.
  • Finalizes with a request for God's will, through Christ, and a concluding "Amen".

Human Histology (Lecture) MT120225 - Unit 1: Introduction to Histology

  • Course offered at UST General Santos - School of Health Sciences, Department of Medical Technology.
  • This is a second-semester course, AY 2024-2025.

Course Content

  • Introduction to Human Histology
  • Tissue Processing
  • Examination of Histologic Slide

Unit Intended Learning Outcomes

  • Students will comprehend fundamental concepts of human histology and how they relate to medical technology.
  • Students will be able to describe different tissue processing methods.

Introduction to Human Histology

  • Histology is the science of microscopic anatomy.
  • It examines the microscopic structures of tissues and organs in the body.

Significance of Histology to MedTech Profession (Philippines)

  • RMTs assist pathologists in the lab.
  • Tissue samples are prepared and processed.

Significance of Histology to MedTech Profession (United States)

  • Can be a histotechnician HT(ASCP) or histotechnologist HTL(ASCP)
  • Histotechnician prepares and processes tissue samples using routine techniques.
  • Histotechnologist performs both routine and complex histologic techniques.
  • Requirements for a career (in the US): obtaining an associate/bachelor's degree or completing a histologic technician program; certification from ASCP.

Levels of Organization in the Human Body

  • (Note: The provided image has a diagram showing the levels of organization.)
  • The order of progressively more complex organization is provided in the slide but without text.

Steps of Tissue Processing

  • A procedure that prepares tissues for microscopic examination.
  • The specific steps are listed below.
    • Fixation
    • Dehydration
    • Clearing
    • Infiltration
    • Embedding
    • Trimming
    • Sectioning
    • Water Bath
    • Staining
    • Mounting

Examination of Histologic Tissue

  • Techniques in handling histology samples for microscope examination.
  • Types of sections (including longitudinal, transverse, oblique)

Examination of a Histological Slide in Light Microscope

  • Artifacts, errors in preparation, are visible on specimen images.

References

  • Specific Histology Textbooks by Mescher, Ross & Pawlina, and Eroschenko are cited.

Dominican Blessing

  • A blessing, or prayer, to invoke God's guidance and blessings for a future undertaking.

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