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Questions and Answers
What is the typical color of the 10x objective lens?
What is the typical color of the 10x objective lens?
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Which of the following WBCs is a granulocyte?
Which of the following WBCs is a granulocyte?
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What is the average lifespan of a red blood cell?
What is the average lifespan of a red blood cell?
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Which type of microscope is used to study the surface of solid objects?
Which type of microscope is used to study the surface of solid objects?
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What is the name of the study of cells?
What is the name of the study of cells?
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What is the wavelength range for visible light?
What is the wavelength range for visible light?
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What type of microscope is commonly used by students in microscopy?
What type of microscope is commonly used by students in microscopy?
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What type of microscope is preferred for observing translucent and colorless specimens?
What type of microscope is preferred for observing translucent and colorless specimens?
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What type of microscope uses fluorophore to generate fluorescence in a biological sample?
What type of microscope uses fluorophore to generate fluorescence in a biological sample?
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What instrument is used for tissue sectioning?
What instrument is used for tissue sectioning?
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What color is 4x objective lens?
What color is 4x objective lens?
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What color is 40x objective lens?
What color is 40x objective lens?
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What color is 100x objective lens?
What color is 100x objective lens?
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Which of the following WBC is not a granulocyte?
Which of the following WBC is not a granulocyte?
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It is used to view detailed structures of cells.
It is used to view detailed structures of cells.
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9it controls the amount of light reaching the specimen.
9it controls the amount of light reaching the specimen.
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It is used to collect and focus the light from the illuminator on to the specimen.
It is used to collect and focus the light from the illuminator on to the specimen.
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What is the average fixation time for most specimens?
What is the average fixation time for most specimens?
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What type of microscope is used to observe more defined ultrastructural intracellular details of individual cells?
What type of microscope is used to observe more defined ultrastructural intracellular details of individual cells?
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What microscope is used to identify the causative agent of syphilis Treponema pallidum?
What microscope is used to identify the causative agent of syphilis Treponema pallidum?
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The image appears dark against a bright contrast/background
The image appears dark against a bright contrast/background
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What is the common fixative agent?
What is the common fixative agent?
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What is the dehydrating agent?
What is the dehydrating agent?
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What is the clearing agent?
What is the clearing agent?
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What is the agent used for both infiltration and embedding?
What is the agent used for both infiltration and embedding?
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What stains lipids?
What stains lipids?
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What is the method used for detecting the presence of a specific protein that can assist with tumor classification and diagnosis?
What is the method used for detecting the presence of a specific protein that can assist with tumor classification and diagnosis?
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What antigens diagnose tumors of epithelial origin?
What antigens diagnose tumors of epithelial origin?
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Breast duct cell tumors
Breast duct cell tumors
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Preserves the tissue structure & prevent degradation by enzymes released from the cells or microorganisms
Preserves the tissue structure & prevent degradation by enzymes released from the cells or microorganisms
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The process of removing the dehydrating agent and is replaced with an organic solvent miscible with both alcohol and the embedding medium
The process of removing the dehydrating agent and is replaced with an organic solvent miscible with both alcohol and the embedding medium
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At what temperature does melted paraffin wax is placed in the oven?
At what temperature does melted paraffin wax is placed in the oven?
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Acid dyes dyes the ___ part of the cell.
Acid dyes dyes the ___ part of the cell.
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It is the method for localizing cellular structures using a specific enzymatic activity present in those structures.
It is the method for localizing cellular structures using a specific enzymatic activity present in those structures.
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It gives rise to all tissue types of the fetus.
It gives rise to all tissue types of the fetus.
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the process where the cells express sets of genes that mediate specific
cytoplasmic activities, becoming efficiently organized in tissues with specialized functions and usually changing their shape accordingly.
the process where the cells express sets of genes that mediate specific cytoplasmic activities, becoming efficiently organized in tissues with specialized functions and usually changing their shape accordingly.
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Cell eating
Cell eating
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cell drinking
cell drinking
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Carbohydrate metabolism
Carbohydrate metabolism
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Protein synthesis
Protein synthesis
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Completes posttranslational modifications of proteins produced in RER then packages and addresses these proteins to their proper destinations
Completes posttranslational modifications of proteins produced in RER then packages and addresses these proteins to their proper destinations
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Synthesizes most ATP during aerobic cellular respiration
Synthesizes most ATP during aerobic cellular respiration
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Maintains intracellular structural support and organization of cells and participates in cell division and facilitates movement.
Maintains intracellular structural support and organization of cells and participates in cell division and facilitates movement.
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Maintain cell shape; support microvilli
Maintain cell shape; support microvilli
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Maintain cell shape and rigidity; organize and move organelles
Maintain cell shape and rigidity; organize and move organelles
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The stage where cell copies all the DNA in its nucleus.
The stage where cell copies all the DNA in its nucleus.
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The stage where the cell divides its copied DNA and cytoplasm to make TWO new cells.
The stage where the cell divides its copied DNA and cytoplasm to make TWO new cells.
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Has abundant ECM
Has abundant ECM
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Small amount of ECM
Small amount of ECM
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Very small amount of ECM
Very small amount of ECM
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Where does most papillae found?
Where does most papillae found?
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Specialized structure used for absorption
Specialized structure used for absorption
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Most other cell types have at least 1 short projection
Most other cell types have at least 1 short projection
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Lining of vessels
Lining of vessels
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Covering thyroid
Covering thyroid
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Lining of intestine
Lining of intestine
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Epidermis
Epidermis
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Bladder, urethers
Bladder, urethers
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Conjunctiva
Conjunctiva
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Nasal cavity
Nasal cavity
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Urethra
Urethra
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Salivary glands
Salivary glands
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Study Notes
Microscope Objectives
- 4x objective lens is colored yellow
- 10x objective lens is colored blue
- 40x objective lens is colored red
- 100x objective lens is colored blue
White Blood Cells (WBCs)
- Lymphocyte is not an agranulocyte
- Lymphocyte is not a granulocyte
- Eosinophil is a granulocyte
- Monocyte is not a granulocyte
- Basophil is a granulocyte
Red Blood Cells (RBCs)
- RBCs have a lifespan of 120 days
Microscopy
- Bright-field microscope is not used to identify the causative agent of syphilis Treponema pallidum
- Dark-field microscope is used to identify the causative agent of syphilis Treponema pallidum
- Phase-contrast microscope is not used to identify the causative agent of syphilis Treponema pallidum
- Average thickness of tissue sectioning is unknown
- Histology is the study of tissues
- Cytology is the study of cells
Electron Microscopy
- Scanning Electron Microscope (SEM) is used to directly study surfaces of solid objects
- Transmission Electron Microscope (TEM) makes use of a beam of electrons transmitted through a specimen to produce a 2D image
- SEM is not used to produce a 2D image of a specimen
Microscope Types
- Bright-field microscope produces an image that appears dark against a bright contrast/background
- Dark-field microscope produces an image that appears bright against a dark contrast/background
- Phase-contrast microscope produces an image that appears with a different phase contrast
- Confocal microscope uses a pinhole to focus light onto a definite spot in the sample and reject out-of-focus fluorescent glare
- Confocal microscope produces a nearly 3D shadowed image under very oblique illumination
Light and Microscopy
- Visible light has a range of wavelength of 400-700 nm
- Bright-field microscope is the most commonly used by students in microscopy
- Dark-field microscope is preferred when observing translucent and colorless specimens
- Fluorescence microscope uses fluorophore to generate fluorescence in a biological sample
- Electron microscope is used to observe more defined ultrastructural intracellular details of individual cells
- Polarizing microscope is not used to observe more defined ultrastructural intracellular details of individual cells
- Confocal microscope is not used to observe more defined ultrastructural intracellular details of individual cells
Laboratory Procedures
- Average fixation time for most specimens is 24 hours
- Microtome is used for tissue sectioning
- Microscope is not used for tissue sectioning
- Paraffin wax is not used for tissue sectioning
- Condenser is used to collect and focus the light from the illuminator onto the specimen
- Iris diaphragm controls the amount of light reaching the specimen
- Objective lens is not used to control the amount of light reaching the specimen
- HPO (High Power Objective) is used to view detailed structures of cells
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Description
Review questions for the Human Histology Laboratory Prelims exam, covering topics such as microscope types and visible light wavelengths. Not a cheat sheet, but a study aid for exam preparation. Useful for students of histology and laboratory sciences.
