Questions and Answers
What is the typical color of the 10x objective lens?
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Which of the following WBCs is a granulocyte?
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What is the average lifespan of a red blood cell?
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Which type of microscope is used to study the surface of solid objects?
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What is the name of the study of cells?
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What is the wavelength range for visible light?
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What type of microscope is commonly used by students in microscopy?
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What type of microscope is preferred for observing translucent and colorless specimens?
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What type of microscope uses fluorophore to generate fluorescence in a biological sample?
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What instrument is used for tissue sectioning?
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What color is 4x objective lens?
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What color is 40x objective lens?
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What color is 100x objective lens?
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Which of the following WBC is not a granulocyte?
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It is used to view detailed structures of cells.
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9it controls the amount of light reaching the specimen.
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It is used to collect and focus the light from the illuminator on to the specimen.
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What is the average fixation time for most specimens?
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What type of microscope is used to observe more defined ultrastructural intracellular details of individual cells?
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What microscope is used to identify the causative agent of syphilis Treponema pallidum?
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The image appears dark against a bright contrast/background
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What is the common fixative agent?
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What is the dehydrating agent?
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What is the clearing agent?
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What is the agent used for both infiltration and embedding?
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What stains lipids?
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What is the method used for detecting the presence of a specific protein that can assist with tumor classification and diagnosis?
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What antigens diagnose tumors of epithelial origin?
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Breast duct cell tumors
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Preserves the tissue structure & prevent degradation by enzymes released from the cells or microorganisms
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The process of removing the dehydrating agent and is replaced with an organic solvent miscible with both alcohol and the embedding medium
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At what temperature does melted paraffin wax is placed in the oven?
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Acid dyes dyes the ___ part of the cell.
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It is the method for localizing cellular structures using a specific enzymatic activity present in those structures.
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It gives rise to all tissue types of the fetus.
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the process where the cells express sets of genes that mediate specific cytoplasmic activities, becoming efficiently organized in tissues with specialized functions and usually changing their shape accordingly.
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Cell eating
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cell drinking
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Carbohydrate metabolism
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Protein synthesis
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Completes posttranslational modifications of proteins produced in RER then packages and addresses these proteins to their proper destinations
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Synthesizes most ATP during aerobic cellular respiration
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Maintains intracellular structural support and organization of cells and participates in cell division and facilitates movement.
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Maintain cell shape; support microvilli
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Maintain cell shape and rigidity; organize and move organelles
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The stage where cell copies all the DNA in its nucleus.
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The stage where the cell divides its copied DNA and cytoplasm to make TWO new cells.
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Has abundant ECM
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Small amount of ECM
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Very small amount of ECM
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Where does most papillae found?
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Specialized structure used for absorption
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Most other cell types have at least 1 short projection
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Lining of vessels
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Covering thyroid
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Lining of intestine
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Epidermis
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Bladder, urethers
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Conjunctiva
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Nasal cavity
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Urethra
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Salivary glands
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Study Notes
Microscope Objectives
- 4x objective lens is colored yellow
- 10x objective lens is colored blue
- 40x objective lens is colored red
- 100x objective lens is colored blue
White Blood Cells (WBCs)
- Lymphocyte is not an agranulocyte
- Lymphocyte is not a granulocyte
- Eosinophil is a granulocyte
- Monocyte is not a granulocyte
- Basophil is a granulocyte
Red Blood Cells (RBCs)
- RBCs have a lifespan of 120 days
Microscopy
- Bright-field microscope is not used to identify the causative agent of syphilis Treponema pallidum
- Dark-field microscope is used to identify the causative agent of syphilis Treponema pallidum
- Phase-contrast microscope is not used to identify the causative agent of syphilis Treponema pallidum
- Average thickness of tissue sectioning is unknown
- Histology is the study of tissues
- Cytology is the study of cells
Electron Microscopy
- Scanning Electron Microscope (SEM) is used to directly study surfaces of solid objects
- Transmission Electron Microscope (TEM) makes use of a beam of electrons transmitted through a specimen to produce a 2D image
- SEM is not used to produce a 2D image of a specimen
Microscope Types
- Bright-field microscope produces an image that appears dark against a bright contrast/background
- Dark-field microscope produces an image that appears bright against a dark contrast/background
- Phase-contrast microscope produces an image that appears with a different phase contrast
- Confocal microscope uses a pinhole to focus light onto a definite spot in the sample and reject out-of-focus fluorescent glare
- Confocal microscope produces a nearly 3D shadowed image under very oblique illumination
Light and Microscopy
- Visible light has a range of wavelength of 400-700 nm
- Bright-field microscope is the most commonly used by students in microscopy
- Dark-field microscope is preferred when observing translucent and colorless specimens
- Fluorescence microscope uses fluorophore to generate fluorescence in a biological sample
- Electron microscope is used to observe more defined ultrastructural intracellular details of individual cells
- Polarizing microscope is not used to observe more defined ultrastructural intracellular details of individual cells
- Confocal microscope is not used to observe more defined ultrastructural intracellular details of individual cells
Laboratory Procedures
- Average fixation time for most specimens is 24 hours
- Microtome is used for tissue sectioning
- Microscope is not used for tissue sectioning
- Paraffin wax is not used for tissue sectioning
- Condenser is used to collect and focus the light from the illuminator onto the specimen
- Iris diaphragm controls the amount of light reaching the specimen
- Objective lens is not used to control the amount of light reaching the specimen
- HPO (High Power Objective) is used to view detailed structures of cells
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