Unit 1_ Intro to Human Histology PDF

Prayer before study Lord, true source of light and wisdom, give me a keen sense of understanding, a retentive memory and the capacity to grasp things correctly. Grant me the grace to be accurate in my expositions and the skill to express myself with thoroughness and clarity. Be with me at the start...

Prayer before study Lord, true source of light and wisdom, give me a keen sense of understanding, a retentive memory and the capacity to grasp things correctly. Grant me the grace to be accurate in my expositions and the skill to express myself with thoroughness and clarity. Be with me at the start of my work, guide its progress and bring it to completion. Grant this through Christ our Lord. Amen.  Human Histology (Lecture) MT120225 UNIT 1: INTRODUCTION TO HISTOLOGY UST General Santos - School of Health Sciences Department of Medical Technology Second Semester A.Y. 2024-2025 Course Content I. INTRODUCTION TO HUMAN HISTOLOGY II. TISSUE PROCESSING III. EXAMINATION OF HISTOLOGIC SLIDE Unit Intended Learning Outcomes: At the end of the unit, the student must be able to: Understand the fundamental concepts of human histology and its relevance to the Medical Technology profession. Describe the various methods involved in tissue processing. 01 | Introduction to Human Histology  Histology ○ Histo = tissue + logia = science ○ Microscopic anatomy ○ Scientific study of microscopic structures of tissues and organs of the body  Significance of Histology to MedTech Profession In the Philippines RMTs assist pathologists in the laboratory Preparing and processing tissue sample  Significance of Histology to MedTech Profession In the United States Can be histotechnician HT(ASCP) or histotechnologist HTL(ASCP) ○ Histotechnician - Prepares and processes tissue samples for routine histologic techniques (fixation, processing, embedding, microtomy, staining) ○ Histotechnologist - Performs both routine and complex histologic techniques (enzyme histochemistry, immunohistochemistry, in-situ hybridization, immunofluorescence)  Significance of Histology to MedTech Profession In the United States Can be histotechnician HT(ASCP) or histotechnologist HTL(ASCP) ○ Requirements Education: obtaining associate or bachelor’s degree or completing an accredited histologic technician program Certification: certification form ASCP REVIEW Levels of Organization in the Human Body O_______ O____ ______ O____ T_____ C___ C_______ 02 | Tissue Processing  1. FIXATION Small pieces of tissue are placed in solutions of chemical that cross-link proteins and inactivate degradative enzymes, which preserve cell and tissue structure. Aim: preserve the tissue nearest to its living state Commonly used fixative: formalin  2. DEHYDRATION The tissue is transferred through a series of increasingly concentrated alcohol solutions, ending in 100% alcohol which removes all water. Aim: remove water molecule of the tissue Commonly used dehydrating agent: ethanol  3. CLEARING Dehydrating agent is removed and replaced by an organic solvent to facilitate the transition of dehydration and infiltration stage. Aim: removal of dehydrating agent, make tissue clear and improve microscopic exam Commonly used clearing agent: Xylene  4. INFILTRATION The tissue is then places in melted medium until it becomes completely infiltrated. AIM: to provide support to the tissue, permit thin sectioning Commonly used medium: Paraffin wax  5. EMBEDDING The infiltrated tissue is placed in a small mold with melted media and allowed to harden. AIM: to provide support to the tissue, permit thin sectioning Commonly used medium: Paraffin wax  6. TRIMMING The resulting paraffin block is trimmed to expose the tissue for sectioning on a microtome.  7. SECTIONING Uses microtome that cuts tissue specimen into thin paraffin ribbon that can be placed on a slide for microscopy. 7. SECTIONING  8. WATER BATH The tissue ribbon is floated on a water bath to flatten it, then fished onto a microscope slide coated with Meyer’s egg albumin as an adhesive.  9. STAINING The tissue will subsequently be stained to visualize different tissue components. Commonly used stain: Hematoxylin and Eosin  10. MOUNTING A mounting agent will then be added to the specimen and a thin glass coverslip placed over the specimen to secure it and prevent damage. Commonly used agent: Canada balsam https://youtu.be/P0cZKCfyUwE?feature=shared https://youtu.be/KnMdSgd5mts?feature=shared MEMORY CHECK!! Steps of Tissue Processing… Tissue Processing “FeDe Ca? Important Event To Sa Waterfront and Sa Moa” 1. Fixation 2. Dehydration 3. Clearing 4. Infiltration 5. Embedding 6. Trimming 7. Sectioning 8. Water Bath 9. Staining 10. Mounting 03 | Examination of Histologic Tissue Examination of a Histological Slide in Light Microscope Examination of a Histological Slide in Light Microscope Artifacts: error in the preparation process  Human Histology (Lecture) MT120225 UNIT 1: INTRODUCTION TO HISTOLOGY UST General Santos - School of Health Sciences Department of Medical Technology Second Semester A.Y. 2024-2025 References Mescher, A. (2023). Junqueira’s Basic Histology Text and Atlas (17th ed). McGraw Hill. Ross, M. and Pawlina, W. (2023). Histology: A Text and Atlas: With Correlated Cell and Molecular Biology. LWW. Eroschenko, V, P. (2013). difiore's Atlas of Histology : With Functional Correlations (12th ed). New Delhi:Wolters Kluwer. Dominican Blessing May God the Father bless us May God the Son heal us May God the Holy Spirit enlighten us Give us eyes to see with, ears to hear with, hands to do the work of God with, feet to walk with, and mouth to preach the word of salvation with; And may the angel of peace watch over us, and lead us at last by the Lord’s gift of the kingdom. Amen.