Histopathology Lab Protocol

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What is the primary consequence of incomplete dehydration on tissue specimens?

Tissue becomes more receptive to infiltration
Tissue becomes more susceptible to chemical damage
Tissue becomes hard, brittle and shrunken
Tissue becomes soft and non receptive to infiltration (correct)

What is the typical concentration range of ethanol used in the initial stage of dehydration in the paraffin wax method?

60%-70% ethanol (correct)
100% ethanol
80%-90% ethanol
40%-50% ethanol

What is the primary factor that determines the choice of dehydrant in tissue preparation?

The type of embedding medium used
The properties of the tissue being processed
The nature of the task and economic factors (correct)
The type of fixative employed

What is the consequence of using a dehydrant that causes excessive tissue shrinkage?

Impaired tissue morphology (C) Signup and view all the answers

What is the purpose of minimizing the duration of dehydration in tissue preparation?

To minimize the risk of tissue damage (A) Signup and view all the answers

What is the exception to the requirement for dehydration in tissue preparation?

Tissues externally supported by an aqueous embedding medium (D) Signup and view all the answers

What is the primary use of gelatin in histology?

In frozen sectioning and production of whole organ sections (B) Signup and view all the answers

Why is the use of celloidin or LVN discouraged in neuropathology?

Due to special requirements needed to house the processing reagents (D) Signup and view all the answers

What is the temperature requirement for holding paraffin wax?

2-4°C above its melting point (C) Signup and view all the answers

What is the purpose of infiltrating tissues with a supporting medium in double embedding?

To support the tissue during embedding (B) Signup and view all the answers

What is the purpose of the cold plate in the embedding process?

To rapidly cool the wax (A) Signup and view all the answers

What is the purpose of the paraffin dispenser?

To dispense the wax automatically into a mold (C) Signup and view all the answers

What is the primary purpose of adding a small drop of detergent to the water bath during paraffin sectioning?

To allow the section to flatten out with greater ease (D) Signup and view all the answers

What is the ideal temperature of the water in the flotation bath during paraffin sectioning?

10°C below the melting point of the paraffin (D) Signup and view all the answers

Which of the following is NOT a type of section adhesive used in histology?

Paraffin wax (A) Signup and view all the answers

What is the purpose of the microtome in histological specimen preparation?

To cut the tissue block into thin sections (A) Signup and view all the answers

What is the primary advantage of using 100% alcohol in the overnight processing schedule?

It allows for faster dehydration of the tissue (C) Signup and view all the answers

What is the most commonly used size of slides in histology?

76 × 25 mm (B) Signup and view all the answers