18 Questions
What is the primary consequence of incomplete dehydration on tissue specimens?
Tissue becomes soft and non receptive to infiltration
What is the typical concentration range of ethanol used in the initial stage of dehydration in the paraffin wax method?
60%-70% ethanol
What is the primary factor that determines the choice of dehydrant in tissue preparation?
The nature of the task and economic factors
What is the consequence of using a dehydrant that causes excessive tissue shrinkage?
Impaired tissue morphology
What is the purpose of minimizing the duration of dehydration in tissue preparation?
To minimize the risk of tissue damage
What is the exception to the requirement for dehydration in tissue preparation?
Tissues externally supported by an aqueous embedding medium
What is the primary use of gelatin in histology?
In frozen sectioning and production of whole organ sections
Why is the use of celloidin or LVN discouraged in neuropathology?
Due to special requirements needed to house the processing reagents
What is the temperature requirement for holding paraffin wax?
2-4°C above its melting point
What is the purpose of infiltrating tissues with a supporting medium in double embedding?
To support the tissue during embedding
What is the purpose of the cold plate in the embedding process?
To rapidly cool the wax
What is the purpose of the paraffin dispenser?
To dispense the wax automatically into a mold
What is the primary purpose of adding a small drop of detergent to the water bath during paraffin sectioning?
To allow the section to flatten out with greater ease
What is the ideal temperature of the water in the flotation bath during paraffin sectioning?
10°C below the melting point of the paraffin
Which of the following is NOT a type of section adhesive used in histology?
Paraffin wax
What is the purpose of the microtome in histological specimen preparation?
To cut the tissue block into thin sections
What is the primary advantage of using 100% alcohol in the overnight processing schedule?
It allows for faster dehydration of the tissue
What is the most commonly used size of slides in histology?
76 × 25 mm
This quiz covers the steps involved in the histopathology laboratory process, including tissue preparation, dehydration, and paraffin embedding. It also covers the equipment required for microtome cutting and the overnight processing schedule. Test your knowledge of the histopathology lab protocol!
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