Histopathology Laboratory Procedures Quiz
48 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What does the accession number represent for a patient?

  • A unique identifier for the patient's billing information
  • A code that indicates the type of specimen collected
  • A unique identifier for the patient, modified for multiple specimens (correct)
  • A number assigned to track laboratory test results

    • When more than one cassette is required for a specimen, what additional information is included?

  • The date and time of specimen collection
  • The pathologist’s signature for approval
  • An additional distinguishing digit for identification (correct)
  • The patient’s full name and address

    • Which of the following tasks is assigned to the laboratory technologist during gross dissection?

  • Selecting the pathologist's slides for review
  • Performing diagnostic analyses on the specimens
  • Updating the LIS system with case notes (correct)
  • Conducting follow-up tests on previous specimens

    • What is the purpose of a computer-generated worksheet in the laboratory?

    <p>To guide the work related to a specific specimen</p> Signup and view all the answers

    What is a critical consequence of errors in specimen identification?

    <p>Inaccurate diagnostic findings</p> Signup and view all the answers

    What might the technologist be required to do for simple cases during grossing?

    <p>Assist in the block selection process</p> Signup and view all the answers

    Which of the following is typically included in the updates made to the LIS after gross dissection?

    <p>Number of blocks selected for each specimen</p> Signup and view all the answers

    What role does the pathologist play during the grossing of specimens?

    <p>Describing the case and selecting relevant blocks</p> Signup and view all the answers

    What is the primary focus of histopathology?

    <p>The study of disease through histological abnormalities</p> Signup and view all the answers

    Which of the following best describes the role of a histotechnologist?

    <p>Preparing tissue specimens for microscopic examination</p> Signup and view all the answers

    What is a key requirement for the accessioning of a tissue specimen?

    <p>Accurate labeling and documentation are essential</p> Signup and view all the answers

    Which safety precaution is critical during gross dissection?

    <p>Wearing gloves and goggles</p> Signup and view all the answers

    What is the purpose of tissue cassettes in laboratory procedures?

    <p>To safely transport and hold tissue samples during processing</p> Signup and view all the answers

    Which equipment is typically used for gross dissection in a laboratory?

    <p>Scalpel and forceps</p> Signup and view all the answers

    What aspect of tissue specimens is primarily described in gross description?

    <p>The visible characteristics and sizes of tissues</p> Signup and view all the answers

    Why are specimens sent to the laboratory in a clinical setting?

    <p>To provide essential diagnostic information</p> Signup and view all the answers

    What is a key feature of a Laboratory Information System (LIS) in modern laboratories?

    <p>It replaces physical record-keeping ledgers.</p> Signup and view all the answers

    Which of the following is NOT a major requirement for specimen labeling?

    <p>Test procedures ordered</p> Signup and view all the answers

    What should be verified by the laboratory worker during specimen accessioning?

    <p>The specimen contains all required clinical data.</p> Signup and view all the answers

    Which unique identifier is NOT mentioned as mandatory for specimen labeling?

    <p>Social Security Number</p> Signup and view all the answers

    Why might pathology samples not be rejected despite not meeting the minimum acceptance criteria?

    <p>They may still be usable for certain types of testing.</p> Signup and view all the answers

    Which of the following is considered a minor requirement for specimen documentation?

    <p>Date of Birth (DOB)</p> Signup and view all the answers

    Which statement about specimen labeling is correct?

    <p>Label and requisition information must match exactly.</p> Signup and view all the answers

    What is the purpose of documenting the fixation time of a specimen?

    <p>It provides crucial information regarding specimen handling.</p> Signup and view all the answers

    What is the primary purpose of using a mechanical pencil in a potentially infectious area?

    <p>To ensure a sharp, fine point is always available</p> Signup and view all the answers

    Which of the following is part of the standard protocol for labeling cassettes?

    <p>S surgical - year - case # - block #</p> Signup and view all the answers

    What additional designation may be used if there is more than one specimen taken?

    <p>An additional digit after the block number</p> Signup and view all the answers

    What should be done if an error is made on a cassette label?

    <p>Completely erase the error</p> Signup and view all the answers

    What are disposable blades designated for after use?

    <p>Biohazardous waste disposal</p> Signup and view all the answers

    What additional information may occasionally be recorded on the long side of the cassette?

    <p>Embedding and cutting instructions</p> Signup and view all the answers

    What is the significance of the label size for cassettes being 28 mm x 8 mm?

    <p>It is essential for legibility and clear writing</p> Signup and view all the answers

    Which automated system is mentioned for marking cassettes?

    <p>VANTAGE system</p> Signup and view all the answers

    What is required for all specimens that are not specifically exempted?

    <p>They must be submitted to the pathology department for examination.</p> Signup and view all the answers

    When will a microscopic examination be performed?

    <p>When the attending physician requests it or when indicated by findings.</p> Signup and view all the answers

    Which of the following is NOT a source of tissue specimens?

    <p>Blood sample</p> Signup and view all the answers

    What is the first step in the pathway of a surgical specimen?

    <p>Specimen arrival at lab</p> Signup and view all the answers

    What does the touch preparation method examine?

    <p>The architectural arrangement of a tissue specimen.</p> Signup and view all the answers

    Which of the following is part of the technical preparatory steps in specimen handling?

    <p>Specimen preparation</p> Signup and view all the answers

    What happens during the initial pathologist viewing?

    <p>Both gross and microscopic examinations are conducted.</p> Signup and view all the answers

    Which of the following statements regarding specimen filing is incorrect?

    <p>Stained slides are not part of specimen filing.</p> Signup and view all the answers

    What type of forceps are useful for removing specimens from deep containers?

    <p>Extra-long forceps</p> Signup and view all the answers

    Why is it important to rinse or wash forceps between cases?

    <p>To avoid contamination of specimens</p> Signup and view all the answers

    What is the primary purpose of marking resection margins on a specimen?

    <p>To aid the pathologist in reconstruction of the specimen</p> Signup and view all the answers

    Which type of ink is most commonly used for marking specimens?

    <p>Permanent ink</p> Signup and view all the answers

    What should be done to help set the ink on the specimen after marking?

    <p>Put it in Bouin’s fluid or a 4% acetic acid solution</p> Signup and view all the answers

    What is the significance of the weight of a specimen in gross dissection?

    <p>It can provide important diagnostic information</p> Signup and view all the answers

    Which agent may be used to color very tiny biopsy specimens for embedding?

    <p>Eosin</p> Signup and view all the answers

    What happens if the marking ink is still wet when the specimen is cut?

    <p>It may obscure the margins</p> Signup and view all the answers

    Study Notes

    Outcome 3: Preparation of Clinical Specimens for Histological Examination

    • This outcome describes the preparation of clinical specimens for histological examination.
    • The process involves specimen acceptance, gross description, gross dissection, fixation, and tissue processing.
    • Different sections of the document define various aspects of the preparation process, including definitions, specimen sources, pathways, preparation methods, and the role of the histotechnologist.

    Section 1: Specimen Acceptance, Gross Description, and Gross Dissection

    • Definitions: Histology, pathology, histopathology, histotechnical technique, histochemistry, and cytology are defined.
    • Specimen Sources: Specimens may originate from autopsy, surgical tissue (whole organ, partial organ, excised tumor, excised cyst), curettage, amputation, products of conception, biopsy, or exfoliated cells.
    • Pathway of a Surgical Specimen: The specimen's journey through the laboratory is outlined, describing stages like identification, accessioning, gross examination, preparation, pathologist viewing, staining, and consultation.
    • Specimen Preparation: Methods for specimen preparation in the lab are listed .
    • Tissue Examination Methods: Details of tissue examination, such as gross examination and use of brightfield microscopy.
    • Role of the Histotechnologist: The histotechnologist's role in specimen entry, gross description, and gross dissection is explained.
    • Specimen Accessioning: The steps involved in accurately recording specimen information to ensure proper identification and tracking are described.
    • Gross Description: The process of gross examination and description of tissue specimens in the clinical laboratory are discussed with details.
    • Gross Dissection: Details of the general procedure for gross dissection, relevant procedures, safety precautions, equipment, and specimen transportation are described.
    • Equipment for Gross Dissection: A description and function of equipment like safety equipment, boards/trays, dissecting tools (scalpels, knives, scissors), and the use of marking reagents for dissection are noted.
    • Safety Precautions: Safety precautions, such as proper use of protective gear and ventilation procedures are noted to avoid accidents and prevent exposure to harmful chemicals.

    Section 2: Fixation

    • Definition and Purpose of Fixation: Fixation is the preservation of tissue to prevent changes after death.
    • Autolysis and Putrefaction: These are defined and their appearance described microscopically.
    • Criteria for a Good Fixative: A good fixative preserves tissue morphology, maintains intracellular components, and prepares the tissue for staining.
    • Causes of Improper Fixation: The document lists causes of improper fixation, such as inadequate fixative volume, incorrect fixative type, or prolonged fixation times
    • Chemical Fixation Methods Different chemical fixation methods, such as vapor fixation, direct immersion, perfusion, are described.
    • Factors Involved in Fixation: Various factors influencing fixation, such as temperature, pH, and speed of penetration are described.
    • Classification of Chemicals: Chemicals used for fixation are classified based on their effect on proteins (additive or non-additive, coagulating or non-coagulating).
    • Specific Fixatives and Their Properties: Properties of various chemicals such as formaldehyde, mercuric chloride, alcohol, picric acid, and acetic acid are described.
    • Properties Unique to Formaldehyde: Formaldehyde's properties, including its use in fixation, tolerance to tissue, and reaction with proteins, are discussed.
    • Compound and Microanatomical Fixatives: Definitions and properties of compound and microanatomical fixatives are provided.
    • Specific Fixative Descriptions: Further detail on neutral buffered formalin, zinc formalin, Lillie's B-5 fixative, and Bouin's fluid, including their ingredients, properties, and applications.
    • Secondary Fixation: Secondary fixation for various purposes and its importance are noted.
    • Fixation Artifacts: The document defines fixation artifacts as structures that are not naturally present in living tissue.
    • Pigments from Fixation: Specific pigments produced by various fixation procedures and how to remove them are identified.

    Section 3: Principles of Tissue Processing

    • Introduction to Processing: Initial steps of processing include dehydration, clearing, and infiltration, are described.
    • Dehydration: Removal of water from tissue specimens
    • Clearing Agents: The removal of alcohol (dehydration) and introduction of wax solvents (clearing).
    • Xylene, Toluene, and Benzene: Comparing clearing agents based on their properties, speed, tolerance, etc.
    • Xylene Substitutes: Alternatives to xylene, relevant information, and hazard data are presented.
    • Universal Solvents: Types of solvents that are miscible with both water and paraffin, relevant information, and hazard data are presented.
    • Factors Affecting Dehydration and Clearing: Various factors, such as the clearing agent, temperature, and the tissue, are noted.
    • Problems in Dehydration and Clearing: Potential issues like incomplete wax infiltration and the brittleness of the tissue.
    • Infiltration: Details on paraffin infiltration and its importance in handling tissue.
    • Commercial Paraffin Waxes: Detailed information on several types of paraffin waxes and their important properties and additives.
    • Factors Affecting Infiltration: Factors that influence the efficiency of infiltration (e.g., tissue size and type, clearing agent used).
    • Vacuum Infiltration: The use of vacuum for more efficient infiltration and advantages of this technique..
    • Automatic Tissue Processing This process is discussed with details on mechanisms like the Fluid Exchange Tissue Processor and modern automatic types.
    • Tissue-Tek V.I.P. Vacuum Infiltration Processor: Specific information about this processor, including its components and functionalities.
    • Recycling Reagents: Materials, procedures, and equipment for recycling tissue-processing reagents are explained.

    Section 4: Decalcification

    • Purpose of Decalcification: Removal of calcium salts from tissue specimens.
    • Criteria for a Good Decalcifying Solution: Requirements for an ideal decalcifying agent.
    • Procedure for Handling Tissue Requiring Decalcification: A step-by-step process for the application and removal of decalcifying agents
    • Types of Bone Specimens: Different types of bone specimens and procedures for handling them.
    • Decalcifying Fluids: Comparison of commercial decalcifying fluids like Nitric Acid, Formic Acid, and EDTA.
    • Endpoint Determination: Testing procedures for decalcification completion .
    • Surface Decalcification: This involves surface-only decalcification, which helps when dealing with tissues having localized calcification.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    Outcome 3 Guide PDF

    Description

    Test your knowledge on essential laboratory procedures in histopathology. This quiz covers topics such as specimen identification, gross dissection roles, and safety precautions. Perfect for students and professionals in the field of histotechnology.

    More Like This

    Use Quizgecko on...
    Browser
    Open
    Browser
    Browser