Podcast
Questions and Answers
What is the recommended volume of formalin for specimen fixation?
What is the recommended volume of formalin for specimen fixation?
20 times the size of the specimen
A patient’s name can be used as a unique accession number.
A patient’s name can be used as a unique accession number.
False
What should be done if a specimen is contaminated or leaking?
What should be done if a specimen is contaminated or leaking?
What is the maximum duration recommended for tissue fixation?
What is the maximum duration recommended for tissue fixation?
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The ____ should be checked for completeness of the sample and compatibility with the surgery done.
The ____ should be checked for completeness of the sample and compatibility with the surgery done.
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What is the primary purpose of logging a specimen?
What is the primary purpose of logging a specimen?
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Which types of specimens should ideally be placed in a wide mouth container or thick plastic bag?
Which types of specimens should ideally be placed in a wide mouth container or thick plastic bag?
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When sending a specimen for culture, it should be placed in a ____ container.
When sending a specimen for culture, it should be placed in a ____ container.
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Study Notes
The Histopathology Laboratory
- Aims to:
- Process tissue samples
- Provide accurate diagnoses
- Archive tissue samples and reports
- Requirements:
- Equipment and technical support for maintenance
- Skilled histotechnologists
- Anatomic Pathologists
- Safety measures and waste disposal systems
Receiving of Specimen
- Complete patient and clinical data is essential for:
- Timely evaluation and reporting
- Contacting patient and doctor
- Outpatient referrals require the submission of:
- Tissue samples, paraffin blocks, slides and histopathology report
- Doctor's request for clinical impression and special preparation or fixation
- Charges are assessed based on the specimen submitted.
Specimen Rejection
- Specimens are rejected for:
- Discrepancies between requisition and specimen label
- Unlabeled or mislabeled specimens
- Contaminated specimens or leaking containers
- Lack of clinical data
- Inappropriately identified specimens
Checking of Specimen
- Check the completeness of specimen:
- Compare type and number of samples with surgery details
- Check laterality (left or right side)
- Check fixation:
- If unfixed: add formalin (surgical) or alcohol (cytology)
- If inadequate or incorrect fixative: add or replace fixative
Specimen Collection and Containers
- Large samples:
- Place in a wide-mouthed container or thick plastic bag with 10% neutral buffered formalin immediately.
- Use a formalin volume 20 times the size of the specimen.
- Very large specimens:
- Send immediately to the laboratory
- Specimens requiring cultures:
- Put 1 part in a sterile container and send to the laboratory immediately
- Immerse the other part in 10% neutral buffered formalin.
Logging of Specimen
- Provide a unique histopathology accession number for each case:
- Surgical cases: S-07-000X
- Cytology cases: C-07-000X
- Avoid using patient's name as the accession number to maintain confidentiality.
- All samples from one surgery should have the same accession number.
- This ensures homogenous evaluation and reporting.
Gross Evaluation of Specimen
- Ideally, tissue should be fixed for 6-48 hours before sectioning for optimal fixative penetration.
- Collect information on the accession number, number of sections and blocks taken per case, and embedding method.
- Samples for gross description:
- Submitted for documentation purposes
- Microscopic examination and histopathologic diagnoses are not performed.
- Special requests:
- Return abortus or calculi to patient
Tissue for Gross Description Only
- Prepuce/foreskin
- Vaginal mucosa
- Scars/cicatrix
- Foreign bodies (e.g., bullets, orthopaedic implants, medical devices)
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Description
This quiz covers essential aspects of the histopathology laboratory, including the processing of tissue samples, accurate diagnoses, and specimen requirements. Understand the importance of proper specimen handling and patient data for effective laboratory evaluation. Explore the key roles and safety measures necessary for maintaining a high-quality histopathology service.
