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Questions and Answers
Which staining technique is used to detect and visualize calcium deposits in tissues?
What is the purpose of using Liquid paraffin and OCT compound in tissue preparation?
What is the purpose of PERL'S stain?
What is the RNA degradation window?
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What is the main purpose of tissue microarrays?
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Which of the following is true about singleplex testing?
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What is the basis of the RNA Scope technique?
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Where should the calibrator be placed for standardization purposes?
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Which type of biopsy is considered one of the most difficult to work on?
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Which packages are compatible with Leica Aperio AT2 and Akoya Phenoimager for analyzing pathological images?
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Study Notes
Calcium Detection
- Alizarin red staining is used to detect and visualize calcium deposits in tissues.
Tissue Preparation
- Liquid paraffin is utilized to preserve and embed tissues for examination under a microscope.
- OCT (Optimal Cutting Temperature) compound aids in freezing tissues for cryosectioning, maintaining cellular morphology.
PERL'S Stain
- PERL'S stain specifically identifies and visualizes iron deposits within tissues, essential for diagnosing conditions like hemochromatosis.
RNA Degradation Window
- The RNA degradation window refers to the timeframe within which RNA begins to degrade, which can affect downstream applications and analyses.
Tissue Microarrays
- The main purpose of tissue microarrays is to enable high-throughput analysis and comparison of multiple tissue samples on a single slide, facilitating research and diagnostics.
Singleplex Testing
- Singleplex testing is characterized by the analysis of one target at a time, providing specific information but limiting the breadth of data compared to multiplex testing.
RNA Scope Technique
- The RNA Scope technique is based on in situ hybridization, allowing for the detection of RNA within cells while preserving tissue architecture.
Calibrator Placement
- For standardization purposes, the calibrator should be placed in a consistent location within the assay to ensure accurate and reproducible results.
Difficult Biopsy Types
- Bone marrow biopsy is considered one of the most difficult types of biopsy to perform due to its invasive nature and complexity.
Compatible Analysis Packages
- Leica Aperio AT2 and Akoya Phenoimager are compatible with various pathology analysis packages, facilitating the evaluation of pathological images in research and clinical settings.
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Description
Test your knowledge of histological techniques and staining methods with this quiz. Learn about the use of liquid paraffin and OCT compound, special stains like PERL'S and Van Kossa, and the RNA degradation window. Explore the world of histoenzymology and its role in revealing proteins in cellular compartments.
