Histological Techniques and Staining Methods Quiz

Calcium Detection

• Alizarin red staining is used to detect and visualize calcium deposits in tissues.

Tissue Preparation

• Liquid paraffin is utilized to preserve and embed tissues for examination under a microscope.
• OCT (Optimal Cutting Temperature) compound aids in freezing tissues for cryosectioning, maintaining cellular morphology.

PERL'S Stain

• PERL'S stain specifically identifies and visualizes iron deposits within tissues, essential for diagnosing conditions like hemochromatosis.

RNA Degradation Window

• The RNA degradation window refers to the timeframe within which RNA begins to degrade, which can affect downstream applications and analyses.

Tissue Microarrays

• The main purpose of tissue microarrays is to enable high-throughput analysis and comparison of multiple tissue samples on a single slide, facilitating research and diagnostics.

Singleplex Testing

• Singleplex testing is characterized by the analysis of one target at a time, providing specific information but limiting the breadth of data compared to multiplex testing.

RNA Scope Technique

• The RNA Scope technique is based on in situ hybridization, allowing for the detection of RNA within cells while preserving tissue architecture.

Calibrator Placement

• For standardization purposes, the calibrator should be placed in a consistent location within the assay to ensure accurate and reproducible results.

Difficult Biopsy Types

• Bone marrow biopsy is considered one of the most difficult types of biopsy to perform due to its invasive nature and complexity.

Compatible Analysis Packages

• Leica Aperio AT2 and Akoya Phenoimager are compatible with various pathology analysis packages, facilitating the evaluation of pathological images in research and clinical settings.