Histology Staining Techniques Quiz
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Questions and Answers

What color is associated with the Cresyl violet acetate method when staining H.pylori?

  • Golden yellow
  • Blue-violet (correct)
  • Dark blue
  • Brown-black

Which staining method would you use to identify spirochetes in a histological preparation?

  • Grocott Methenamine Silver
  • Toluidine blue
  • Levaditi’s method (correct)
  • Orcein method

What is the characteristic color of the Warthin-Starry method when staining spirochetes?

  • Bright red
  • Gray-black
  • Black (correct)
  • Red

Which color indicates the presence of maturating RBCs when using the Grocott Methenamine Silver method?

<p>Yellow (D)</p> Signup and view all the answers

In the Giemsa stain, what color is associated with mast cell granules?

<p>Deep blue (A)</p> Signup and view all the answers

What is the main color result when using the PAS stain?

<p>Magenta red (A)</p> Signup and view all the answers

Which stain is considered obsolete for glycogen staining?

<p>Langhan’s iodine method (D)</p> Signup and view all the answers

What color does Alcian blue stain acid mucins?

<p>Blue (D)</p> Signup and view all the answers

Which stain is specifically used for Crytococcus neoformans?

<p>Mucicarmine stain (B)</p> Signup and view all the answers

Which of the following techniques results in a dark blue result for acidic mucins?

<p>Colloidal iron technique (C)</p> Signup and view all the answers

What is the color result of Sudan IV when staining triglycerides?

<p>Brilliant red (C)</p> Signup and view all the answers

Which stain is known to have a limited duration of effectiveness, lasting only for 2 hours?

<p>Acridine orange (D)</p> Signup and view all the answers

What color result is obtained when staining with gomori’s aldehyde fuchsin for sulfated mucins?

<p>Purple (B)</p> Signup and view all the answers

What color indicates the presence of gangliosides when using the Borohydride-Periodic-Schiff method?

<p>Red (D)</p> Signup and view all the answers

Which method results in a brownish-black deposit indicating the presence of alkaline phosphatase?

<p>Gomori calcium method (A), Gomori calcium method (D)</p> Signup and view all the answers

What is the resulting color of protamines when stained using the alkaline fast-green method?

<p>Green (C)</p> Signup and view all the answers

What color is expected when performing Sakaguchi’s test for arginine?

<p>Orange-red (C)</p> Signup and view all the answers

Which staining method is used to visualize acid phosphatase, and what is its resulting color?

<p>Gomori lead method; Black (A)</p> Signup and view all the answers

What color is produced when using the Weil’s method on myelin?

<p>Black (B)</p> Signup and view all the answers

Which stain produces a purple coloration when staining lipofuscin?

<p>Gomori’s aldehyde fuchsin (D)</p> Signup and view all the answers

What is the end result color when using the Gram-Twort stain on Gram (-) organisms?

<p>Pink-red (B)</p> Signup and view all the answers

Which stain method gives a dark blue result when staining bile pigments?

<p>Schmorl’s ferric ferricyanide method (D)</p> Signup and view all the answers

What color result is typically observed in the Masson Fontana technique when staining melanin?

<p>Black (A)</p> Signup and view all the answers

What color do bile pigments yield when subjected to the Lindquist modified rhodanine technique?

<p>Red to orange-red (A)</p> Signup and view all the answers

Which of the following stains identifies hemosiderin with a deep blue color?

<p>Perl’s Prussian blue (C)</p> Signup and view all the answers

When using the Gmelin technique, what initial color is observed before it changes to green?

<p>Blue-purple (A)</p> Signup and view all the answers

What does the Indoxyl acetate method specifically stain?

<p>Nonspecific esterase (A)</p> Signup and view all the answers

Which staining method is known to provide the most reliable identification of DNA?

<p>Feulgen technique (C)</p> Signup and view all the answers

What color is produced by the Methyl green-pyronin stain for RNA?

<p>Red (C)</p> Signup and view all the answers

What does the Gomori’s silver impregnation stain primarily target?

<p>Reticulin fibers (B)</p> Signup and view all the answers

Which stain shows collagen and mucus resulting in a blue color?

<p>Masson’s trichrome stain (B)</p> Signup and view all the answers

What color does the Weigert’s stain produce for elastic fibers?

<p>Dark-blue/blue-black (C)</p> Signup and view all the answers

The Bodian’s stain is primarily used for the diagnosis of which condition?

<p>Alzheimer’s disease (C)</p> Signup and view all the answers

What result does the Congo red stain yield when staining amyloid?

<p>Red (C)</p> Signup and view all the answers

Which staining method is noted for staining muscle fibers red?

<p>Modified Gomori’s Trichrome stain (C)</p> Signup and view all the answers

Which stain is indicated for staining Nissl granules and nucleoli in deep blue?

<p>Toluidine blue (A)</p> Signup and view all the answers

What color result does the Martius-Scarlet-Blue stain yield for muscle?

<p>Red (C)</p> Signup and view all the answers

Which staining technique is associated with elastic fibers resulting in a black coloration?

<p>Verhoeff’s stain (A)</p> Signup and view all the answers

What type of tissue does the Azocarmine stain primarily target?

<p>Connective tissue (C)</p> Signup and view all the answers

Which staining method yields a purple result for Nissl substance?

<p>Cresyl fast violet (B)</p> Signup and view all the answers

Flashcards

PAS Stain

A staining technique used to identify carbohydrates like glycogen, mucins, and components of bacteria and fungi, and basement membrane by reacting to the aldehyde groups in these substances.

PAS with Diastase Control

A modified PAS staining procedure used to differentiate glycogen from other polysaccharides by removing glycogen with diastase before staining, thus highlighting just the glycogen.

Best Carmine

A specific stain used for highlighting glycogen. It's selective and highly specific for glycogen.

Alcian Blue

A stain used primarily to highlight acid mucins, resulting in a blue color.

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Sudan Black

A stain used to visualize lipids, appearing as dark blue-black.

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Oil Red O

A stain specifically for lipids, producing a brilliant red color.

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Nile Blue Sulfate

A stain used to identify different types of lipids by their varying colors under light (neutral fat, cholesterol esters, fatty acids).

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Acridine Orange

A stain used for acid mucins and MPSs (mucopolysaccharides) and fungi; shows fluorescence under UV light. Fungi show a greenish fluorescence.

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Cerebrosides Stain

Cerebrosides are stained light blue using neutral lipid stains.

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Sulfatide Stain

Sulfatides are stained with toluidine blue-acetone, showing a metachromatic red-brown or yellow color.

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Gangliosides Stain

Gangliosides are stained red using a borohydride-periodic-Schiff method.

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Histones Stain

Histones are stained green using alkaline fast-green.

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Acid Phosphatase Stain

Acid phosphatase is stained black using the Gomori lead method.

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Neurons Stain

A staining method used to visualize neurons using pale purple-blue colors, highlighting their structure.

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Myelin Sheath Staining

A staining method used to reveal myelin sheaths through blue-black, blue-green, or black colors, showcasing their structure, thickness, and location.

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Hemosiderin Staining

A staining technique to detect hemosiderin, a golden-brown iron-containing pigment, appearing as deep blue when stained.

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Bile Pigment Staining

A set of staining methods to detect bile pigments, resulting in staining colors ranging from emerald, blue-green, blue-purple, and green, depending on the method, oxidation, and pigment.

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Gram-Twort Stain

Used to differentiate bacteria based on their cell wall composition: gram-positive appear blue-black, gram-negative appear pink-red.

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Lipofuscin Staining

Used to visualize lipofuscin (age pigment) with a purple color, using Gomori's aldehyde fuchsin method.

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Melanin Staining

Staining of melanin pigment; appear black when stained with Fontana-Masson techniques because melanin reduces ammoniacal silver solutions.

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Calcium Staining

Detection of calcium using Von Kossa; stained tissue appears black

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Levaditi's method

A staining technique used to visualize spirochetes, appearing as black on a yellowish background.

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Grocott Methenamine Silver

Used for staining fungi, resulting in sharply outlined black staining.

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Toluidine blue

A stain that highlights H. pylori with a dark blue color against a blue background.

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Cresyl violet acetate method

Used to stain H. pylori, resulting in a blue-violet color.

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Warthin-Starry method

Staining method that highlights spirochetes in black.

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Feulgen technique

A staining technique used to specifically identify DNA.

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Methyl green-pyronin

A stain used to differentiate RNA and DNA.

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Gomori's silver impregnation

A stain that highlights reticulin fibers in tissues.

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Van Gieson's Stain

A stain that highlights collagen, and other components like muscle and RBCs.

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Masson's trichrome

A stain used to differentiate collagen, muscle, and other tissue components.

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Mallory's aniline blue

Stain showing connective tissue fibers.

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Azocarmine

A stain used to highlight connective tissue (CT).

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Weigert's stain

A stain that specifically targets elastic fibers.

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Verhoeff's stain

A method for staining elastic tissue a black color.

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Congo red

A stain used to identify amyloid.

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Modified Gomori's trichrome

A stain used primarily to highlight muscle fibers.

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Lissamine fast red

Highlights specific muscle and RBC types after staining.

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Schmorl's Picro-Thionin

Staining technique for bone lacunae and canaliculi.

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Bielschowsky's technique

A stain that helps visualize neurofibrils, axons, and dendrites.

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Bodian's stain

Diagnoses Alzheimer's disease by highlighting nerve fibers and nerve endings in tissue sections.

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Study Notes

Staining Techniques and Results

  • PAS: Stains CHO, glycogen, mucins, bacteria, and fungi. Positive result is magenta red.
  • PAS with diastase control: Stains glycogen, showing a positive result as red if glycogen is present.
  • Best Carmine: Stains glycogen, giving a bright red result.
  • Langhan's iodine method: Stains glycogen, producing a mahogany brown result.
  • Alcian Blue: Stains acid mucins, resulting in a blue stain.
  • Alcian Blue-PAS: Differentiates between acid and neutral mucins. Acid mucins stain blue, and neutral mucins magenta.
  • Gomori's aldehyde fuchsin stain: Stains mucins, showing a red result for mucins, and purple for sulfated mucins, and blue for carboxylated mucins.
  • Cryptococcus neoformans stain: Results in a red stain.
  • Colloidal (Dialyzed) iron technique: Stains acid mucins, resulting in dark blue.
  • Acridine orange: Stains acid mucins/MPS dark blue & fungi give a greenish red fluorescence.
  • Sudan black B: Stains lipids, appearing as blue-black.
  • Sudan IV (Scharchlach R): Stains lipids, appearing red.
  • Oil red O: Stains lipids, appearing red.
  • Osmium tetroxide: Stains lipids, producing a brilliant red product.
  • Nile blue sulfate method: Stains neutral fat, cholesterol esters, fatty acids, and cerebrosides. Results in different colors for different kinds of lipids (pinkish red, light red, deep blue to violet, and light blue).
  • Toluidine blue-acetone method: Differentiates sulfatide, showing a metachromatic red-brown color.
  • Borohydride-Periodic Schiff method: Stains gangliosides.
  • Alkaline fast green method: Stains histones, giving a green result.
  • Peracetic acid-Alcian blue: Stains histones, protamines, and cystines.
  • Sakaguchi’s test: Stains arginine, appearing red.
  • Gomori calcium method, Gomori lead method, lead method, metal precipitation, Calcium cobalt method, and a-naphthyl acetate method: Various stains for different components, ranging in color results, from black to reddish brown.

Additional Staining Techniques and Results

  • Indoxyl acetate method: Stains nonspecific esterase, appearing bluish-black.
  • Tetrazolium method: Stains monoamine oxidase, showing a purplish-red result.
  • Feulgen technique: Stains DNA, showing a reddish-purple result.
  • Methyl green-pyronin: Stains RNA, showing red for nucleoli and green for chromatin.
  • Gomori's silver impregnation stain: Stains reticulin fibers, giving a black result.
  • Van Gieson's stain: Stains collagen and mucus, appearing pink/deep red and yellow.
  • Masson's trichrome stain: Stains collagen, mucus, cytoplasm fibers, and RBC, resulting in different colors for the different components (blue, red).
  • Mallory's aniline blue: Differentiates collagen, cytoplasm and fibroglia , staining them red, giving an excellent stain showing CT fibers,
  • Azocarmine: Stains elastic fibers, appearing pale pink/yellow, and RBC with a yellow result as well.
  • Weigert's, Verhoeff's, Taenzer-Unna-Orcein, and Krajian's technique: Stains elastic fibers, resulting in various colors including dark blue, black, dark-brown, and bright red,
  • Martius-Scarlet-Blue: Stains muscle and collagen, including RBCs. showing blue, red, and other colors.
  • Mallory’s PTAH: Stains fibrin, showing either dark-blue or blue on the stained solution, as well as muscle striations, neuroglia, and amoebas, appearing as different shades of blue.
  • Congo red: Stains amyloid, appearing red or purplish red.
  • Methyl violet-crystal violet method: Stains amyloid, resulting in a purplish red color.
  • Thioflavin-T fluorescent staining: Stains amyloid, showing a yellowish fluorescence.
  • Modified Gomori's trichrome stain and Lissamine fast red: Staining different components for different muscle and collagen fibers, showing red and green results for various components.
  • Schmorl's Picro-Thionin method and Bielschowsky's technique: Stains lacunae and canaliculi in the bone matrix, showing dark-brown/black and yellow/brownish yellow colors. Stains neurofibrils, axons, and dendrites also with different results depending on the component (black on a grayish background or lightly strained).
  • Bodian's, Sevier-Munger, Toluidine blue, polychrome methylene blue, Thionine, Cresyl fast violet, Weigert-Pal, Luxol fast blue, Cajal's gold sublimate, Perl’s and Gomori’s Prussian blue, Turnbull’s blue , Benzidine-nitroprusside stain, Mod. Fouchet's technique, Gmelin technique, Stein's iodine, Schmorl's ferric... and many other stains: Various techniques and stains are used for a large variety of components in the tissue samples, with various results, such as darkening, discoloration or different hues.
  • Gram-Twort, Brown & Brenn, Wade-Fite, Toluidine blue..., Cresyl violet acetate, Dieterle, etc. Various techniques and stains target bacteria, spirochetes, etc., with different colors.
  • Lendrum's phloxine-tartrazine, Orcein, Giemsa: Various stains for different cellular components, showing different results such as brown black or bright red.

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Description

Test your knowledge on the various histological staining techniques and their results. This quiz covers essential stains like PAS, Alcian Blue, and Best Carmine, providing insight into how they identify specific components in tissue samples. Enhance your understanding of histology with this informative quiz!

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