Podcast
Questions and Answers
What color is associated with the Cresyl violet acetate method when staining H.pylori?
What color is associated with the Cresyl violet acetate method when staining H.pylori?
Which staining method would you use to identify spirochetes in a histological preparation?
Which staining method would you use to identify spirochetes in a histological preparation?
What is the characteristic color of the Warthin-Starry method when staining spirochetes?
What is the characteristic color of the Warthin-Starry method when staining spirochetes?
Which color indicates the presence of maturating RBCs when using the Grocott Methenamine Silver method?
Which color indicates the presence of maturating RBCs when using the Grocott Methenamine Silver method?
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In the Giemsa stain, what color is associated with mast cell granules?
In the Giemsa stain, what color is associated with mast cell granules?
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What is the main color result when using the PAS stain?
What is the main color result when using the PAS stain?
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Which stain is considered obsolete for glycogen staining?
Which stain is considered obsolete for glycogen staining?
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What color does Alcian blue stain acid mucins?
What color does Alcian blue stain acid mucins?
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Which stain is specifically used for Crytococcus neoformans?
Which stain is specifically used for Crytococcus neoformans?
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Which of the following techniques results in a dark blue result for acidic mucins?
Which of the following techniques results in a dark blue result for acidic mucins?
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What is the color result of Sudan IV when staining triglycerides?
What is the color result of Sudan IV when staining triglycerides?
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Which stain is known to have a limited duration of effectiveness, lasting only for 2 hours?
Which stain is known to have a limited duration of effectiveness, lasting only for 2 hours?
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What color result is obtained when staining with gomori’s aldehyde fuchsin for sulfated mucins?
What color result is obtained when staining with gomori’s aldehyde fuchsin for sulfated mucins?
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What color indicates the presence of gangliosides when using the Borohydride-Periodic-Schiff method?
What color indicates the presence of gangliosides when using the Borohydride-Periodic-Schiff method?
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Which method results in a brownish-black deposit indicating the presence of alkaline phosphatase?
Which method results in a brownish-black deposit indicating the presence of alkaline phosphatase?
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What is the resulting color of protamines when stained using the alkaline fast-green method?
What is the resulting color of protamines when stained using the alkaline fast-green method?
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What color is expected when performing Sakaguchi’s test for arginine?
What color is expected when performing Sakaguchi’s test for arginine?
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Which staining method is used to visualize acid phosphatase, and what is its resulting color?
Which staining method is used to visualize acid phosphatase, and what is its resulting color?
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What color is produced when using the Weil’s method on myelin?
What color is produced when using the Weil’s method on myelin?
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Which stain produces a purple coloration when staining lipofuscin?
Which stain produces a purple coloration when staining lipofuscin?
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What is the end result color when using the Gram-Twort stain on Gram (-) organisms?
What is the end result color when using the Gram-Twort stain on Gram (-) organisms?
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Which stain method gives a dark blue result when staining bile pigments?
Which stain method gives a dark blue result when staining bile pigments?
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What color result is typically observed in the Masson Fontana technique when staining melanin?
What color result is typically observed in the Masson Fontana technique when staining melanin?
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What color do bile pigments yield when subjected to the Lindquist modified rhodanine technique?
What color do bile pigments yield when subjected to the Lindquist modified rhodanine technique?
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Which of the following stains identifies hemosiderin with a deep blue color?
Which of the following stains identifies hemosiderin with a deep blue color?
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When using the Gmelin technique, what initial color is observed before it changes to green?
When using the Gmelin technique, what initial color is observed before it changes to green?
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What does the Indoxyl acetate method specifically stain?
What does the Indoxyl acetate method specifically stain?
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Which staining method is known to provide the most reliable identification of DNA?
Which staining method is known to provide the most reliable identification of DNA?
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What color is produced by the Methyl green-pyronin stain for RNA?
What color is produced by the Methyl green-pyronin stain for RNA?
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What does the Gomori’s silver impregnation stain primarily target?
What does the Gomori’s silver impregnation stain primarily target?
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Which stain shows collagen and mucus resulting in a blue color?
Which stain shows collagen and mucus resulting in a blue color?
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What color does the Weigert’s stain produce for elastic fibers?
What color does the Weigert’s stain produce for elastic fibers?
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The Bodian’s stain is primarily used for the diagnosis of which condition?
The Bodian’s stain is primarily used for the diagnosis of which condition?
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What result does the Congo red stain yield when staining amyloid?
What result does the Congo red stain yield when staining amyloid?
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Which staining method is noted for staining muscle fibers red?
Which staining method is noted for staining muscle fibers red?
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Which stain is indicated for staining Nissl granules and nucleoli in deep blue?
Which stain is indicated for staining Nissl granules and nucleoli in deep blue?
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What color result does the Martius-Scarlet-Blue stain yield for muscle?
What color result does the Martius-Scarlet-Blue stain yield for muscle?
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Which staining technique is associated with elastic fibers resulting in a black coloration?
Which staining technique is associated with elastic fibers resulting in a black coloration?
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What type of tissue does the Azocarmine stain primarily target?
What type of tissue does the Azocarmine stain primarily target?
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Which staining method yields a purple result for Nissl substance?
Which staining method yields a purple result for Nissl substance?
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Study Notes
Staining Techniques and Results
- PAS: Stains CHO, glycogen, mucins, bacteria, and fungi. Positive result is magenta red.
- PAS with diastase control: Stains glycogen, showing a positive result as red if glycogen is present.
- Best Carmine: Stains glycogen, giving a bright red result.
- Langhan's iodine method: Stains glycogen, producing a mahogany brown result.
- Alcian Blue: Stains acid mucins, resulting in a blue stain.
- Alcian Blue-PAS: Differentiates between acid and neutral mucins. Acid mucins stain blue, and neutral mucins magenta.
- Gomori's aldehyde fuchsin stain: Stains mucins, showing a red result for mucins, and purple for sulfated mucins, and blue for carboxylated mucins.
- Cryptococcus neoformans stain: Results in a red stain.
- Colloidal (Dialyzed) iron technique: Stains acid mucins, resulting in dark blue.
- Acridine orange: Stains acid mucins/MPS dark blue & fungi give a greenish red fluorescence.
- Sudan black B: Stains lipids, appearing as blue-black.
- Sudan IV (Scharchlach R): Stains lipids, appearing red.
- Oil red O: Stains lipids, appearing red.
- Osmium tetroxide: Stains lipids, producing a brilliant red product.
- Nile blue sulfate method: Stains neutral fat, cholesterol esters, fatty acids, and cerebrosides. Results in different colors for different kinds of lipids (pinkish red, light red, deep blue to violet, and light blue).
- Toluidine blue-acetone method: Differentiates sulfatide, showing a metachromatic red-brown color.
- Borohydride-Periodic Schiff method: Stains gangliosides.
- Alkaline fast green method: Stains histones, giving a green result.
- Peracetic acid-Alcian blue: Stains histones, protamines, and cystines.
- Sakaguchi’s test: Stains arginine, appearing red.
- Gomori calcium method, Gomori lead method, lead method, metal precipitation, Calcium cobalt method, and a-naphthyl acetate method: Various stains for different components, ranging in color results, from black to reddish brown.
Additional Staining Techniques and Results
- Indoxyl acetate method: Stains nonspecific esterase, appearing bluish-black.
- Tetrazolium method: Stains monoamine oxidase, showing a purplish-red result.
- Feulgen technique: Stains DNA, showing a reddish-purple result.
- Methyl green-pyronin: Stains RNA, showing red for nucleoli and green for chromatin.
- Gomori's silver impregnation stain: Stains reticulin fibers, giving a black result.
- Van Gieson's stain: Stains collagen and mucus, appearing pink/deep red and yellow.
- Masson's trichrome stain: Stains collagen, mucus, cytoplasm fibers, and RBC, resulting in different colors for the different components (blue, red).
- Mallory's aniline blue: Differentiates collagen, cytoplasm and fibroglia , staining them red, giving an excellent stain showing CT fibers,
- Azocarmine: Stains elastic fibers, appearing pale pink/yellow, and RBC with a yellow result as well.
- Weigert's, Verhoeff's, Taenzer-Unna-Orcein, and Krajian's technique: Stains elastic fibers, resulting in various colors including dark blue, black, dark-brown, and bright red,
- Martius-Scarlet-Blue: Stains muscle and collagen, including RBCs. showing blue, red, and other colors.
- Mallory’s PTAH: Stains fibrin, showing either dark-blue or blue on the stained solution, as well as muscle striations, neuroglia, and amoebas, appearing as different shades of blue.
- Congo red: Stains amyloid, appearing red or purplish red.
- Methyl violet-crystal violet method: Stains amyloid, resulting in a purplish red color.
- Thioflavin-T fluorescent staining: Stains amyloid, showing a yellowish fluorescence.
- Modified Gomori's trichrome stain and Lissamine fast red: Staining different components for different muscle and collagen fibers, showing red and green results for various components.
- Schmorl's Picro-Thionin method and Bielschowsky's technique: Stains lacunae and canaliculi in the bone matrix, showing dark-brown/black and yellow/brownish yellow colors. Stains neurofibrils, axons, and dendrites also with different results depending on the component (black on a grayish background or lightly strained).
- Bodian's, Sevier-Munger, Toluidine blue, polychrome methylene blue, Thionine, Cresyl fast violet, Weigert-Pal, Luxol fast blue, Cajal's gold sublimate, Perl’s and Gomori’s Prussian blue, Turnbull’s blue , Benzidine-nitroprusside stain, Mod. Fouchet's technique, Gmelin technique, Stein's iodine, Schmorl's ferric... and many other stains: Various techniques and stains are used for a large variety of components in the tissue samples, with various results, such as darkening, discoloration or different hues.
- Gram-Twort, Brown & Brenn, Wade-Fite, Toluidine blue..., Cresyl violet acetate, Dieterle, etc. Various techniques and stains target bacteria, spirochetes, etc., with different colors.
- Lendrum's phloxine-tartrazine, Orcein, Giemsa: Various stains for different cellular components, showing different results such as brown black or bright red.
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Description
Test your knowledge on the various histological staining techniques and their results. This quiz covers essential stains like PAS, Alcian Blue, and Best Carmine, providing insight into how they identify specific components in tissue samples. Enhance your understanding of histology with this informative quiz!
