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Questions and Answers
What percentage of normal hemoglobin is typically Hgb A?
What percentage of normal hemoglobin is typically Hgb A?
In a person with sickle cell trait (AS), what is expected in the hemoglobin electrophoresis pattern?
In a person with sickle cell trait (AS), what is expected in the hemoglobin electrophoresis pattern?
What might you observe in a hemoglobin electrophoresis for a person with sickle cell disease (SS)?
What might you observe in a hemoglobin electrophoresis for a person with sickle cell disease (SS)?
What is true of individuals with hemoglobin C disease (CC)?
What is true of individuals with hemoglobin C disease (CC)?
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Individuals with hemoglobin SC disease demonstrate which of the following in their electrophoresis pattern?
Individuals with hemoglobin SC disease demonstrate which of the following in their electrophoresis pattern?
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What is the relationship between the appearance of bands in hemoglobin electrophoresis and the genes present?
What is the relationship between the appearance of bands in hemoglobin electrophoresis and the genes present?
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When assessing hemoglobin electrophoresis, what is a fundamental characteristic of a normal hemoglobin pattern?
When assessing hemoglobin electrophoresis, what is a fundamental characteristic of a normal hemoglobin pattern?
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Why is it important to memorize the characteristics of hemoglobin electrophoresis patterns?
Why is it important to memorize the characteristics of hemoglobin electrophoresis patterns?
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What does the presence of one large band adjacent to the anode indicate?
What does the presence of one large band adjacent to the anode indicate?
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How many bands would you expect to see for a patient with Hemoglobin SC Disease (SC)?
How many bands would you expect to see for a patient with Hemoglobin SC Disease (SC)?
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At what pH level does electrophoresis run to determine hemoglobin types according to the provided content?
At what pH level does electrophoresis run to determine hemoglobin types according to the provided content?
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Which hemoglobin type would show a separation pattern indicating two bands located adjacent to each other?
Which hemoglobin type would show a separation pattern indicating two bands located adjacent to each other?
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If a patient demonstrates only one band at the location where Hemoglobin A is expected, what is the likely diagnosis?
If a patient demonstrates only one band at the location where Hemoglobin A is expected, what is the likely diagnosis?
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What key information can be deduced from the absence of multiple bands for a patient suspected of Sickle Cell Disease?
What key information can be deduced from the absence of multiple bands for a patient suspected of Sickle Cell Disease?
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What role does the position of Hgb F during electrophoresis play in diagnosis?
What role does the position of Hgb F during electrophoresis play in diagnosis?
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In the context provided, what does a band positioned differently in an electrophoresis diagram suggest?
In the context provided, what does a band positioned differently in an electrophoresis diagram suggest?
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What is the primary conclusion drawn from the electrophoresis pattern observed in a patient with Hemoglobin C Disease?
What is the primary conclusion drawn from the electrophoresis pattern observed in a patient with Hemoglobin C Disease?
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Why can Sickle Cell Trait (AS) be suspected in an athlete with no symptoms when tested at pH 8.4?
Why can Sickle Cell Trait (AS) be suspected in an athlete with no symptoms when tested at pH 8.4?
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Which finding allows for the elimination of Sickle Cell Disease (SS) as a diagnosis for the patient in the case?
Which finding allows for the elimination of Sickle Cell Disease (SS) as a diagnosis for the patient in the case?
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At pH 6.2, how would Hemoglobin SC Disease be represented on an electrophoresis pattern?
At pH 6.2, how would Hemoglobin SC Disease be represented on an electrophoresis pattern?
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What characteristic finding differentiates Hemoglobin C Disease from other hemoglobinopathies?
What characteristic finding differentiates Hemoglobin C Disease from other hemoglobinopathies?
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What is the significance of performing hemoglobin electrophoresis at different pH levels?
What is the significance of performing hemoglobin electrophoresis at different pH levels?
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What is the expected electrophoresis pattern for a patient with Sickle Cell Trait at pH 8.4?
What is the expected electrophoresis pattern for a patient with Sickle Cell Trait at pH 8.4?
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In diagnosing hemoglobinopathies, what is primarily assessed through hemoglobin electrophoresis?
In diagnosing hemoglobinopathies, what is primarily assessed through hemoglobin electrophoresis?
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Study Notes
Hemoglobin Electrophoresis Study Notes
- Electrophoresis separates unknown molecules into constituent parts.
- The distinctive pattern created serves as a "fingerprint" for identification.
- Electrophoresis is used in scientific research and daily testing.
- Memorize CSFA+ (pH 8.4) and +CSAF-(pH 6.2) labels for electrophoresis.
- Normal hemoglobin produces one band at the Hgb A position.
- Sickle cell trait (AS) displays equal sized bands at A and S positions.
- Sickle cell disease (SS) shows one fat band at the S position and a thin band at the F position.
- Hemoglobin C disease (CC) demonstrates a large band at the C position.
- Hemoglobin SC disease (SC) displays two equal-sized bands at the S and C positions.
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Description
This quiz covers the principles of hemoglobin electrophoresis, including the identification patterns produced by various hemoglobin types. You'll learn about the distinct bands for normal hemoglobin, sickle cell traits, and other hemoglobinopathies. Understand the significance of pH levels in the separation process.
