Hemoglobin Electrophoresis: Identification of Hemoglobin C and S

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Questions and Answers

What is the primary basis for the separation of hemoglobin molecules in an electric field?

Differences in molecular size
Differences in beta chain function
Variations in alpha chain structure
Differences in total molecular charge (correct)

What is the direction of migration in Hemoglobin Electrophoresis?

From anode to cathode
Randomized migration
From cathode to anode (correct)
No migration occurs

What is the purpose of performing Hemoglobin Electrophoresis in a citrate agar medium?

To confirm the presence of Hemoglobin C and S (correct)
To identify Hemoglobin F
To confirm the presence of Hemoglobin A
To diagnose sickle cell disease

Which of the following hemoglobin types is associated with sickle cell disease?

<p>Hb S and Hb A (A)</p> Signup and view all the answers

What is the charge of the alpha beta chains at the cathode electrode?

<p>Negatively charged (A)</p> Signup and view all the answers

Flashcards

Hemoglobin Electrophoresis Separation Basis

The difference in the total electrical charge of the hemoglobin molecules.

Hemoglobin Electrophoresis Migration Direction

From the negatively charged cathode to the positively charged anode.

Purpose of Citrate Agar in Hemoglobin Electrophoresis

Citrate agar medium is used to specifically identify Hemoglobin C and S. This medium provides optimal conditions for these variants to separate and be clearly recognized.

Hemoglobin Types Associated with Sickle Cell Disease

Hb S (sickle cell hemoglobin) is the primary hemoglobin variant associated with sickle cell disease. Hb A is the normal adult hemoglobin, but in sickle cell disease, it's often present along with Hb S.

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