Podcast
Questions and Answers
Which of the following stains is NOT used for the demonstration of reticulocytes?
Which of the following stains is NOT used for the demonstration of reticulocytes?
What is the N:C ratio of a rubriblast?
What is the N:C ratio of a rubriblast?
Which of the following statements best describes the cytoplasm of a rubriblast?
Which of the following statements best describes the cytoplasm of a rubriblast?
Which feature does NOT characterize the maturation of erythroid precursors?
Which feature does NOT characterize the maturation of erythroid precursors?
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Basophilia in cells is mainly attributed to which factor?
Basophilia in cells is mainly attributed to which factor?
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What happens to the nuclear diameter during the maturation of reticulocyte precursors?
What happens to the nuclear diameter during the maturation of reticulocyte precursors?
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What is the visual assessment used to determine the N:C ratio?
What is the visual assessment used to determine the N:C ratio?
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Which term describes the pinkness of a cell due to the accumulation of basic components?
Which term describes the pinkness of a cell due to the accumulation of basic components?
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Study Notes
Supravital Stains for Reticulocytes
- Supravital stains are used to identify reticulocytes in a Wright-stained smear.
- New methylene blue and brilliant cresyl blue are examples of supravital stains.
Erythroid Precursor Maturation
- As erythroid precursors mature, the overall and nuclear cell diameters decrease.
- Chromatin condenses and clumps.
- Nucleoli disappear.
- Cytoplasm changes from blue to gray-blue then to salmon pink.
N:C Ratio
- N:C ratio is a morphological feature to identify and stage RBC and WBC precursors.
- It's a visual estimate of the nucleus area relative to the cytoplasm area.
- If areas are equal N:C ratio is 1:1
- If nucleus is less than 50% of the cell, N:C ratio is lower (e.g., 1:5).
- If nucleus is more than 50% of the cell, N:C ratio is higher (e.g., 3:1).
Basophilia
- Basophilia refers to the blueness of a particular part of the cell.
- It's due to acidic components attracting basic stains like methylene blue.
- Degree of cytoplasmic basophilia correlates with ribosomal RNA quantity.
Eosinophilia/Acidophilia
- Eosinophilia/Acidophilia is the pinkness of a particular part of a cell.
- It's linked to the accumulation of basic components that attract acid stains like eosin.
- As RBC matures, eosinophilia correlates with hemoglobin accumulation, which decreases basophilia.
Rubriblast (Pronormoblast) Characteristics
- Size: 12-19 μm
- Nucleus: round to slightly oval, thin nuclear membrane, centrally or slightly eccentric.
- Nucleoli: 1-2
- Cytoplasm: small, dark blue, homogenous (due to ribosome concentration).
- N:C ratio: 4:1 (Turgeon)
- This cell (rubriblast) gives rise to prorubricytes.
- Rodak (8:1): very far away, not yet wed.
- Earliest recognizable precursor using light microscopy; not the youngest precursor.
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Description
Explore the essential techniques and concepts in hematology related to supravital stains and erythroid precursor maturation. This quiz covers the characteristics of reticulocytes, the significance of N:C ratio, and the role of basophilia in cell identification. Test your knowledge and understanding of these vital concepts in blood cell analysis.
