Hematology Blood Exam Quiz
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Questions and Answers

What is the conversion factor used to convert from g/dL to g/l?

  • x10 (correct)
  • x100
  • ÷100
  • ÷10
  • What is the normal hematocrit range for adult males?

  • 120-160 g/l
  • 100-140 g/l
  • 130-170 g/l (correct)
  • 160-230 g/l
  • What is the primary use of a hemoglobinometer?

  • Assessing the amount of hemoglobin in a blood sample (correct)
  • Analysing the coagulation time of blood
  • Measuring the volume of red blood cells in a sample of blood
  • Determining the number of white blood cells in blood
  • Which of the following is NOT a method used to determine hematocrit?

    <p>Buffy Coat analysis (A)</p> Signup and view all the answers

    What are two key applications of hemoglobinometer readings?

    <p>Identifying potential blood donors and diagnosing anemia (B)</p> Signup and view all the answers

    When using a macromethod of hematocrit determination, what aspect of the centrifuged blood sample is directly measured?

    <p>The volume of packed red blood cells (A)</p> Signup and view all the answers

    According to the table, which condition could potentially be associated with a higher than normal hematocrit?

    <p>Dehydration (C)</p> Signup and view all the answers

    Hemoglobinometers are utilized in the evaluation of anemia therapies. How does this work?

    <p>Hemoglobinometers track the progression of anemia by monitoring the amount of hemoglobin in the blood (A)</p> Signup and view all the answers

    What is the relationship between hematocrit and hemoglobin?

    <p>Hematocrit is roughly three times the amount of hemoglobin (A)</p> Signup and view all the answers

    Why might a pregnant woman have low hemoglobin but high hematocrit levels?

    <p>Increased blood volume during pregnancy dilutes the hemoglobin concentration causing lower hemoglobin and higher hematocrit levels (C)</p> Signup and view all the answers

    When counting RBCs in the large center square, which of the following directions for counting should be used?

    <p>Snake-like direction from left to right (D)</p> Signup and view all the answers

    What is the depth factor used in the calculation of RBC count?

    <p>10 (C)</p> Signup and view all the answers

    Which of the following is NOT a precaution to take when using a pipette to draw blood?

    <p>Use a mouth pipette (D)</p> Signup and view all the answers

    What is the dilution factor used for calculating the total number of RBCs?

    <p>200 (B)</p> Signup and view all the answers

    When counting WBCs, which of the following squares are counted?

    <p>4 corner squares and center square (B)</p> Signup and view all the answers

    According to the content, what is the function of the RBC pipette and WBC pipette?

    <p>To dilute blood for counting cells (D)</p> Signup and view all the answers

    When counting WBCs, why should cells touching boundary lines be excluded?

    <p>To avoid counting the same cell twice (D)</p> Signup and view all the answers

    What is the purpose of the 'snake-like' direction mentioned when counting WBCs?

    <p>To ensure a consistent counting pattern (A)</p> Signup and view all the answers

    Which of the following best describes the MCV?

    <p>The volume of the average red cell in a given sample of blood, expressed in femtoliters (fl) (B)</p> Signup and view all the answers

    A patient has an MCV of 70 fl. What can be concluded about the patient's red blood cells?

    <p>The patient's red blood cells are smaller than normal. (A)</p> Signup and view all the answers

    Which of the following is NOT a characteristic of red blood cells (RBCs) that is typically measured in a complete blood count (CBC)?

    <p>Mean Platelet Volume (MPV) (A)</p> Signup and view all the answers

    What is the primary function of red blood cells?

    <p>To transport oxygen from the lungs to the tissues and remove carbon dioxide from the tissues to the lungs (B)</p> Signup and view all the answers

    What is the formula used to calculate RBCs per liter?

    <p>N x D/A x 10 x 10^6 (C)</p> Signup and view all the answers

    Which of the following is NOT a condition affecting WBC count?

    <p>Dehydration (D)</p> Signup and view all the answers

    What is the primary reason for the increase in WBC count during exercise?

    <p>Immune system stimulation (A)</p> Signup and view all the answers

    What is the primary reason for leukopenia during viral infections?

    <p>Suppression of the immune system (A)</p> Signup and view all the answers

    What is the normal RBC count range for adult females in RBCs per liter?

    <p>4.0-5.5 x 10^6 (B)</p> Signup and view all the answers

    What does the symbol "N" represent in the formula for RBCs per liter?

    <p>Total number of red blood cells counted (A)</p> Signup and view all the answers

    What is the meaning of "Leukocytopenia"?

    <p>Decrease in white blood cell count (D)</p> Signup and view all the answers

    What is the hematocrit level range for a 6-year-old child?

    <p>34-42% (C)</p> Signup and view all the answers

    What is the hematocrit level range for a male adult?

    <p>42-52% (B)</p> Signup and view all the answers

    What is the purpose of a hemocytometer in a complete blood count (CBC)?

    <p>To count the number of blood cells in a sample (B)</p> Signup and view all the answers

    What is a hemoctyometer used for?

    <p>All of the above (D)</p> Signup and view all the answers

    What does a hemocytometer look like?

    <p>A small, rectangular chamber with a grid pattern (B)</p> Signup and view all the answers

    Flashcards

    Hemoglobinometer

    A device used to measure hemoglobin concentration in blood.

    Hematocrit

    The percentage of blood volume occupied by red blood cells.

    Rule of 3

    Hematocrit value is usually three times the hemoglobin concentration.

    Physiological changes in pregnancy

    Pregnancy may alter hemoglobin and hematocrit levels; often low hemoglobin and high hematocrit.

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    Anemia therapy evaluation

    The process of assessing treatment effectiveness for anemia using hemoglobin measurement.

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    Platelet Count

    A measure of the number of platelets in blood, important for clotting.

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    Hematocrit Ranges

    The percentage of red blood cells in blood, varies by age and sex.

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    Hemocytometer

    A device used for counting blood cells and other cells under a microscope.

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    Sperm and Bacterial Count

    Uses hemocytometer for counting sperm and bacteria in samples.

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    Cerebrospinal Fluid Examination

    Hemocytometer can also evaluate cells in CSF but is referred differently.

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    Normal Hematocrit Range for Newborns

    16-23 g/dL or 160-230 g/L. Typical range for newborns.

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    Normal Hematocrit Range for Children

    10-14 g/dL or 100-140 g/L. Range for children.

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    Normal Hematocrit Range for Adult Males

    13-17 g/dL or 130-170 g/L. Typical range for adult males.

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    Manual Wintrobe Macromethod

    A method for determining hematocrit using a tube and naked eye.

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    High-power objective

    A microscope lens used for detailed viewing, specifically for counting cells.

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    WBC counting method

    Count WBCs in four large corner squares using a specific snake-like approach.

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    RBC counting area

    RBCs are counted in the center square and its corners, usually with a specific pattern.

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    No mouth pipetting

    Safety precaution to prevent ingesting biological samples while using pipettes.

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    Counting direction for WBCs

    Count WBCs from left to right, moving down to the next row in a snake-like direction.

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    Boundary line counting rule

    Cells on the upper and left lines are counted, while cells on bottom and right lines are not.

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    RBC pipette function

    Used to absorb blood and mix it with a diluent for CBC analysis.

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    RBC count reporting formula

    RBC count is the average of counted RBCs multiplied by dilution, depth factor, and area.

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    Mean Corpuscular Volume (MCV)

    Volume of the average red blood cell in blood, expressed in femtoliters.

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    Normal MCV Range

    Normal values for MCV are between 80 to 100 fl or μm3.

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    Mean Corpuscular Hemoglobin (MCH)

    Average amount of hemoglobin per red blood cell, measured in picograms.

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    Normal MCH Range

    Normal values for MCH are between 27 to 32 pg or μg.

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    Mean Corpuscular Hemoglobin Concentration (MCHC)

    Concentration of hemoglobin in a given volume of packed red blood cells, expressed as a percentage.

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    RBC Count Calculation

    Total RBC count is calculated by counting cells and multiplying by 10,000.

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    Leukocytosis

    An increase in WBC count due to infection, leukemia, or exercise.

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    Causes of Leukocytosis

    May occur due to pathologic conditions or physiological responses.

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    Leukopenia

    A decrease in the total number of WBCs, also called leukocytopenia.

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    Causes of Leukopenia

    Can result from viral infections like HIV or chemotherapy.

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    Blood Dilution Factor (D)

    D = dilution of blood in the RBC count formula.

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    Total Area Counted (A)

    A = total area counted in mm² for RBC calculation.

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    Normal RBC Count Range (Females)

    Normal RBC count for females is 4.0-5.5 x 10^6 RBCs/mm³.

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    Study Notes

    Hematologic Exams

    • Hematology is the study of blood and blood-forming tissues, including causes, prognoses, and treatments of blood-related disorders.
    • Blood comprises 6-8% of normal adult body weight, approximately 5 liters.
    • Blood components include plasma (55%), cellular/formed elements (45%), and the buffy coat (less than 1%).
      • Plasma, the liquid portion, is mostly water (91%), containing dissolved gasses, salts, glucose, proteins (like albumin, antibodies, amino acids, and lipids), vitamins, and electrolytes.
      • Cellular/formed elements include Red Blood Cells (RBCs), White Blood Cells (WBCs), and Platelets.
      • Buffy coat: leukocytes (white blood cells) and platelets, less than 1%.

    Hemoglobin Tests

    • Hemoglobin, a protein, gives blood its red color. It carries oxygen via oxyhemoglobin and releases it as deoxyhemoglobin.
    • Hemoglobin measurements assess the oxygen-carrying capacity of the blood.
    • Hemoglobin is an important indicator of blood loss and anemia.
    • Methods for measurement include chemical methods (e.g., cyanmethemoglobin), Sahli method, and others.

    Hematocrit Determination

    • Hematocrit measures the percentage of blood volume occupied by RBCs.
    • It's used to diagnose conditions such as anemia (low hematocrit) and polycythemia (high hematocrit).
    • Methods include manual Wintrobe method and microhematocrit methods.

    Complete Blood Count (CBC)

    • CBC examines blood components including RBCs, WBCs, and platelets.
    • CBC tests include RBC count, WBC count, hemoglobin, hematocrit, and RBC indices.
    • RBC count measures the number of RBCs per unit volume.
    • WBC count measures the number of WBCs per unit volume.

    Red Blood Cell (RBC) Indices

    • Indices like MCV (mean corpuscular volume), MCH (mean corpuscular hemoglobin) and MCHC (mean corpuscular hemoglobin concentration) provide information about the size and hemoglobin content of RBCs.
    • These indices are used in the diagnosis and monitoring of anemias.

    White Blood Cell (WBC) Count

    • WBCs are important components of the immune system.
    • WBC counts are used in diagnosing infections and other conditions.
    • WBC counts should be within a specific range, with deviations indicating possible issues.

    Platelets

    • Platelets are essential for blood clotting and hemostasis.
    • Their low or high counts can indicate conditions requiring medical attention.

    Other Important Information

    • Blood donation removes RBC wastes, causing the body to produce new blood cells.
    • Blood samples are often diluted prior to counting cells. Specialized fluids are used in diluting the blood to avoid harming the cells.
    • Correct methods for counting cells are required to avoid mistakes and errors.

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    Description

    Test your knowledge on hematology, focusing on the study of blood components, their functions, and hemoglobin tests. This quiz covers essential concepts about blood's role in the body, including structures and their significance in health and disease.

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