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Glycolysis Net Reaction

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Questions and Answers

What is the purpose of the first step in glycolysis, which involves phosphorylation of glucose?

To prevent glucose from being transported out of the cell (correct)

To trap glucose in the mitochondria

To generate two molecules of ATP

To convert glucose-6-P to fructose-6-P

Why is the conversion of glucose-6-P to fructose-6-P considered an isomerization reaction?

It leads to the release of carbon dioxide

It results in the formation of water molecules

It involves a change in the arrangement of atoms without losing any atoms (correct)

It involves the generation of energy

Which enzyme is responsible for converting glucose-6-P to fructose-6-P in glycolysis?

Aldolase

Phosphofructokinase

Phosphoglucose isomerase (correct)

Hexokinase

What does the term 'kinases' typically do in biochemical reactions?

Transfer a phosphate to their substrate from ATP Signup and view all the answers

Which enzyme catalyzes the irreversible phosphorylation of fructose-6-P at the second carbon to form fructose-1,6bisphosphate?

Phosphofructokinase Signup and view all the answers

What is the significance of the prefix 'bis' in bisphosphate?

It signifies two separate monophosphates are present. Signup and view all the answers

Which molecule is formed by the cleavage of fructose-1,6-bisphosphate by aldolase?

Glyceraldehyde-3-phosphate and dihydroxyacetone phosphate Signup and view all the answers

What happens to dihydroxyacetone phosphate in the glycolytic pathway?

It is converted to glyceraldehyde-3-phosphate by an isomerase. Signup and view all the answers

What is the significance of a rise in fructose-1,6-bisP in the context of glycolysis?

Clear indication of an increased flux through the pathway Signup and view all the answers

Why does the liver perform glycolysis despite not needing energy for contraction?

To maintain glucose levels in the blood Signup and view all the answers

How does pyruvate kinase activity respond to an increased flux through glycolysis?

Increases to handle the higher flux Signup and view all the answers

What is one major role of glycolysis in the liver according to the text?

To generate reducing power in the form of NADPH Signup and view all the answers

What is the net reaction for the entire glycolysis pathway?

Glucose + 2 Pi +2 ADP + 2 NAD+ 2 pyruvate + 2 ATP + 2 NADH + 2 H+ + 2 H2O Signup and view all the answers

In the formula ΔG = ΔG° + RT ln [C][D]/[A][B], what does ΔG° represent?

The free energy change under standard conditions Signup and view all the answers

Why is glycolysis considered irreversible as a pathway?

Glycolysis only flows in one direction towards pyruvate production Signup and view all the answers

What additional term is adopted for biochemical reactions to refer to the standard free energy change at pH 7?

ΔG°' Signup and view all the answers