Glycolysis Part 1: The 10 Reactions Lecture 20 BCMB 401 Spring Reading: Chapter 16.1-2
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Questions and Answers

What is the main fuel source that almost all organisms use?

  • Lipids
  • Fats
  • Proteins
  • Glucose (correct)

How many transmembrane α-helices do glucose transporters possess?

  • 8
  • 6
  • 12 (correct)
  • 10

In glycolysis, what is the function of NADH?

  • Generate glucose molecules
  • Produce enzyme catalyzed reactions
  • Carry electrons to produce ATP (correct)
  • Transport glucose into the cell

During which phase of glycolysis is glucose converted to glyceraldehyde 3-phosphate (GAP)?

<p>Investment Phase (B)</p> Signup and view all the answers

How many ATP molecules are invested in the conversion of glucose to glyceraldehyde 3-phosphate (GAP) in glycolysis?

<p>2 ATP (D)</p> Signup and view all the answers

What is the end product generated from the conversion of two molecules of GAP during the Payoff Phase of glycolysis?

<p>Pyruvate (D)</p> Signup and view all the answers

How many molecules of NADH are produced from the entire process of glycolysis?

<p>2 (C)</p> Signup and view all the answers

Which type of energy is carried by NADH in glycolysis?

<p>'Chemical' energy (B)</p> Signup and view all the answers

What is the function of Hexokinase in glycolysis?

<p>Phosphorylates glucose to form glucose 6-phosphate (D)</p> Signup and view all the answers

What is the role of Phosphoglucose Isomerase?

<p>Converts aldose sugars to ketose sugars (C)</p> Signup and view all the answers

Why is Phosphofructokinase considered a committed step in glycolysis?

<p>Utilizes the second investment of ATP in stage 1 (C)</p> Signup and view all the answers

What does Aldolase do in glycolysis?

<p>Cleaves a six-carbon molecule into two three-carbon isomers (A)</p> Signup and view all the answers

Why is Triose phosphate isomerase considered important in glycolysis?

<p>Catalyzes a key regulated step in glycolysis (C)</p> Signup and view all the answers

Which enzyme employs substrate-binding induced fit in glycolysis?

<p>Hexokinase (A)</p> Signup and view all the answers

What is the fate of glyceraldehyde 3-phosphate in glycolysis?

<p>Harvests energy in stage 2 (D)</p> Signup and view all the answers

Why is the kcat /KM ratio close to the diffusion-controlled limit for Triose phosphate isomerase?

<p>It catalyzes a reaction that proceeds towards GAP formation (C)</p> Signup and view all the answers

Which enzyme catalyzes the reaction that produces ATP through substrate-level phosphorylation in glycolysis?

<p>Phosphoglycerate Kinase (D)</p> Signup and view all the answers

What is the main purpose of the final three reactions in glycolysis?

<p>To generate high phosphoryl transfer potential compounds (B)</p> Signup and view all the answers

What compound is phosphorylated to make ATP through substrate-level phosphorylation?

<p>ADP (A)</p> Signup and view all the answers

Which process involves the direct transfer of a phosphate to ADP from a phosphorylated intermediate compound?

<p>Substrate-level phosphorylation (D)</p> Signup and view all the answers

What is the net result of glucose catabolism through glycolysis?

<p>Conversion to pyruvate and ATP (A)</p> Signup and view all the answers

Which enzyme catalyzes a thermodynamically favorable reaction in glycolysis?

<p>Glyceraldehyde 3-phosphate Dehydrogenase (A)</p> Signup and view all the answers

What decreases the activation energy barrier in the second half of the Glyceraldehyde 3-phosphate Dehydrogenase-catalyzed reaction?

<p>$ ext{ADP}$ phosphorylation (C)</p> Signup and view all the answers

Which compound has a higher phosphoryl transfer potential than ATP?

<p>$ ext{Thioester}$ (A)</p> Signup and view all the answers

What happens during the formation of ATP by substrate-level phosphorylation in glycolysis?

<p>$ ext{Phosphate}$ is transferred to $ ext{ADP}$ from a phosphorylated compound (C)</p> Signup and view all the answers

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