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Genetics and Genetic Engineering A - Karyotyping Outlines

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Genetics and Genetic Engineering A - Karyotyping Outlines

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Questions and Answers

What effect does treating onion root tips with colchicine have on chromosomes?

Lengthens the chromosomes and enhances spindle formation

Shortens the chromosomes and prevents spindle formation (correct)

Causes chromosomes to duplicate and split

Induces chromosome mutation

How do the chromosomes in colchicine-treated cells differ from those in non-colchicine-treated cells?

Absent during metaphase

Clumped together and irregular in shape

Compact and difficult to distinguish

Spread-out and easily countable (correct)

What is the purpose of using 0.04% colchicine solution in the experiment?

To accelerate cell division

To promote spindle formation

To arrest mitosis (correct)

To induce chromosome replication

How is Colchicine prepared for use in the experiment?

Dissolve 0.04 g of colchicine powder in 100 ml water to create a saturated solution (C) Signup and view all the answers

What is the chromosome number in Allium cepa after being treated with colchicine?

2n = 16 (A) Signup and view all the answers

What is the main effect of Colchicine on mitosis in cells?

Arrests mitosis (C) Signup and view all the answers

How long should the onion bulbs be left in 0.04% colchicine solution during the experiment?

4 hours (C) Signup and view all the answers

Why is it important to fix the onion bulbs after treating them with colchicine?

To preserve cell structure and prevent degradation (C) Signup and view all the answers

What do spread-out chromosomes in a metaphase provide that clumped chromosomes do not?

Easily countable chromosomes (B) Signup and view all the answers