Genetics and Genetic Engineering A - Karyotyping Outlines
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Questions and Answers

What effect does treating onion root tips with colchicine have on chromosomes?

  • Lengthens the chromosomes and enhances spindle formation
  • Shortens the chromosomes and prevents spindle formation (correct)
  • Causes chromosomes to duplicate and split
  • Induces chromosome mutation

How do the chromosomes in colchicine-treated cells differ from those in non-colchicine-treated cells?

  • Absent during metaphase
  • Clumped together and irregular in shape
  • Compact and difficult to distinguish
  • Spread-out and easily countable (correct)

What is the purpose of using 0.04% colchicine solution in the experiment?

  • To accelerate cell division
  • To promote spindle formation
  • To arrest mitosis (correct)
  • To induce chromosome replication

How is Colchicine prepared for use in the experiment?

<p>Dissolve 0.04 g of colchicine powder in 100 ml water to create a saturated solution (C)</p> Signup and view all the answers

What is the chromosome number in Allium cepa after being treated with colchicine?

<p>2n = 16 (A)</p> Signup and view all the answers

What is the main effect of Colchicine on mitosis in cells?

<p>Arrests mitosis (C)</p> Signup and view all the answers

How long should the onion bulbs be left in 0.04% colchicine solution during the experiment?

<p>4 hours (C)</p> Signup and view all the answers

Why is it important to fix the onion bulbs after treating them with colchicine?

<p>To preserve cell structure and prevent degradation (C)</p> Signup and view all the answers

What do spread-out chromosomes in a metaphase provide that clumped chromosomes do not?

<p>Easily countable chromosomes (B)</p> Signup and view all the answers

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