15 Questions
What are the properties of a good host organism for genetic engineering?
A good host organism should be easy to grow and transform, not hinder replication of recombinant vector, not have restriction and methylase activities, be deficient in recombination function, easily retrievable from the transformed host, and be suitable for the specific goal of the genetic engineering process.
What are the different types of transformation vectors mentioned in the text?
The two types of transformation vectors mentioned are cloning vector, used to increase the number of copies of a cloned DNA fragment, and expression vector, used for expression of foreign genes into proteins.
What is a shuttle vector?
A shuttle vector is a vector designed to perform equally in two different hosts.
What are eukaryotic systems favored for in genetic engineering?
Eukaryotic systems are favored for the expression of recombinant proteins that require post-translational modification and if they can grow easily in continuous culture.
What is the role of a vector in genetic engineering?
A vector is an autonomously replicating DNA molecule designed to carry foreign DNA inside a host cell for cloning or expression of foreign genes.
What is a shuttle vector used for in genetic engineering?
Performing equally in two different hosts
Why are eukaryotic systems favored for expression of recombinant proteins?
They require post translational modification for protein expression
What is the main purpose of a cloning vector in genetic engineering?
Increasing the number of copies of a cloned DNA fragment
Why are bacterial hosts favored for genetic engineering over eukaryotic systems?
Bacterial hosts are easy to grow and transform
What type of vector is used for expression of foreign genes into proteins?
Expression vector
Which property is NOT important for a good host organism in genetic engineering?
Ability to hinder replication of recombinant vector
What type of vector is designed to perform equally in two different hosts?
Shuttle vector
In genetic engineering, which type of vector is used for increasing the number of copies of a cloned DNA fragment?
Cloning vector
Why are eukaryotic systems favored for expression of recombinant proteins that require post-translational modification?
They can grow easily in continuous culture
What is the main purpose of an expression vector in genetic engineering?
To express a foreign gene into a protein
Learn about the important properties of a good host organism for genetic engineering, including ease of growth and transformation, lack of hindrance to vector replication, absence of restriction and methylase activities, and deficiency in recombination function. This lecture is presented by Professor Mohamed Abou-Ellail, an expert in Genetics at Aswan University.
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