Genetic Engineering Lecture 7: Choice of Host Organism

A good host organism should be easy to grow and transform, not hinder replication of recombinant vector, not have restriction and methylase activities, be deficient in recombination function, easily retrievable from the transformed host, and be suitable for the specific goal of the genetic engineering process.

The two types of transformation vectors mentioned are cloning vector, used to increase the number of copies of a cloned DNA fragment, and expression vector, used for expression of foreign genes into proteins.

A shuttle vector is a vector designed to perform equally in two different hosts.

<p>Eukaryotic systems are favored for the expression of recombinant proteins that require post-translational modification and if they can grow easily in continuous culture.</p> Signup and view all the answers

<p>A vector is an autonomously replicating DNA molecule designed to carry foreign DNA inside a host cell for cloning or expression of foreign genes.</p> Signup and view all the answers

<p>Performing equally in two different hosts</p> Signup and view all the answers

<p>They require post translational modification for protein expression</p> Signup and view all the answers

<p>Increasing the number of copies of a cloned DNA fragment</p> Signup and view all the answers

<p>Bacterial hosts are easy to grow and transform</p> Signup and view all the answers

<p>Expression vector</p> Signup and view all the answers

<p>Ability to hinder replication of recombinant vector</p> Signup and view all the answers

<p>Shuttle vector</p> Signup and view all the answers

<p>Cloning vector</p> Signup and view all the answers

<p>They can grow easily in continuous culture</p> Signup and view all the answers

<p>To express a foreign gene into a protein</p> Signup and view all the answers