Podcast
Questions and Answers
How are DNA fragments inserted into plasmids or viral vectors?
How are DNA fragments inserted into plasmids or viral vectors?
- Using a gene gun
- Via heat shock treatment (correct)
- Through biolistics
- By electroporation
What is the purpose of Heat Shock in plasmid insertion?
What is the purpose of Heat Shock in plasmid insertion?
- To cool down the cells
- To prevent DNA uptake
- To increase antibiotic resistance
- To expand membrane pores (correct)
What technique uses electric shock to introduce genes into mammalian cells?
What technique uses electric shock to introduce genes into mammalian cells?
- Plasmid insertion
- Biolistics
- Electroporation (correct)
- Heat Shock Treatment
Why do transformed cells survive in the presence of antibiotics?
Why do transformed cells survive in the presence of antibiotics?
What is the function of a selection marker in inserted plasmid DNA?
What is the function of a selection marker in inserted plasmid DNA?
What type of plasmid allows only transformed cells to survive in the presence of antibiotics?
What type of plasmid allows only transformed cells to survive in the presence of antibiotics?
What is the primary purpose of genetic engineering?
What is the primary purpose of genetic engineering?
What is the key difference between classical breeding and genetic engineering?
What is the key difference between classical breeding and genetic engineering?
Which step in the genetic engineering process involves separating DNA fragments by size?
Which step in the genetic engineering process involves separating DNA fragments by size?
What type of enzyme is used in the DNA Cleavage stage of genetic engineering?
What type of enzyme is used in the DNA Cleavage stage of genetic engineering?
What is one of the main applications of genetically modified organisms (GMOs)?
What is one of the main applications of genetically modified organisms (GMOs)?
What is one of the main challenges faced by researchers regarding GMOs?
What is one of the main challenges faced by researchers regarding GMOs?
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