Recent Lessons

Show all results for ""

Genetic Engineering and Plant-Based Products

Choose a study mode

Play Quiz

Study Flashcards

Spaced Repetition

Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of using restriction endonucleases in the production of recombinant DNA?

To increase the pore sizes of the plasma membranes
To identify the specific DNA fragment of interest
To cleave the source DNA and plasmid or viral vectors at the same sites (correct)
To join the DNA fragments to the plasmid or viral vector

What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?

To concentrate the plasmids near the cells
To increase the pore sizes of the plasma membranes
To select for cells that have taken up the plasmid
To allow the plasmid DNA to enter the cells (correct)

What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?

To allow only 'transformed' cells to survive in the presence of the antibiotic (correct)
To increase the pore sizes of the plasma membranes
To concentrate the plasmids near the cells
To select for cells that have taken up the plasmid

What is the purpose of the 'hybridization' step in the screening of recombinant cells?

To identify the cloned genes that form base-pairs with complementary sequences on another nucleic acid (A) Signup and view all the answers

Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?

Biolistics (C) Signup and view all the answers

Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?

Electroporation (A) Signup and view all the answers

What is the main purpose of genetic engineering?

To modify the traits of an organism by introducing new genes or altering gene expression (C) Signup and view all the answers

What is the first stage in the genetic engineering process?

Cleaving the source DNA using a restriction endonuclease (C) Signup and view all the answers

How does genetic engineering differ from classical breeding?

Classical breeding focuses on mating organisms with desirable traits, while genetic engineering modifies traits at the molecular level (A) Signup and view all the answers

Which industries benefit from the application of genetic engineering?

The pharmaceutical, industrial, agricultural, medical, and other industries (A) Signup and view all the answers

What is the purpose of gel electrophoresis in the genetic engineering process?

To separate DNA fragments based on their size (C) Signup and view all the answers

What is a potential challenge faced by researchers in genetic engineering?

Proving the significance of genetically modified organisms (GMOs) as a scientific breakthrough (C) Signup and view all the answers

Flashcards are hidden until you start studying