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Genetic Engineering and Plant-Based Products

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Genetic Engineering and Plant-Based Products

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Questions and Answers

What is the purpose of using restriction endonucleases in the production of recombinant DNA?

To increase the pore sizes of the plasma membranes

To identify the specific DNA fragment of interest

To cleave the source DNA and plasmid or viral vectors at the same sites (correct)

To join the DNA fragments to the plasmid or viral vector

What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?

To concentrate the plasmids near the cells

To increase the pore sizes of the plasma membranes

To select for cells that have taken up the plasmid

To allow the plasmid DNA to enter the cells (correct)

What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?

To allow only 'transformed' cells to survive in the presence of the antibiotic (correct)

To increase the pore sizes of the plasma membranes

To concentrate the plasmids near the cells

To select for cells that have taken up the plasmid

What is the purpose of the 'hybridization' step in the screening of recombinant cells?

To identify the cloned genes that form base-pairs with complementary sequences on another nucleic acid Signup and view all the answers

Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?

Biolistics Signup and view all the answers

Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?

Electroporation Signup and view all the answers

What is the main purpose of genetic engineering?

To modify the traits of an organism by introducing new genes or altering gene expression Signup and view all the answers

What is the first stage in the genetic engineering process?

Cleaving the source DNA using a restriction endonuclease Signup and view all the answers

How does genetic engineering differ from classical breeding?

Classical breeding focuses on mating organisms with desirable traits, while genetic engineering modifies traits at the molecular level Signup and view all the answers

Which industries benefit from the application of genetic engineering?

The pharmaceutical, industrial, agricultural, medical, and other industries Signup and view all the answers

What is the purpose of gel electrophoresis in the genetic engineering process?

To separate DNA fragments based on their size Signup and view all the answers

What is a potential challenge faced by researchers in genetic engineering?

Proving the significance of genetically modified organisms (GMOs) as a scientific breakthrough Signup and view all the answers

Genetic Engineering and Plant-Based Products

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Podcast

Questions and Answers

What is the purpose of using restriction endonucleases in the production of recombinant DNA?

What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?

What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?

What is the purpose of the 'hybridization' step in the screening of recombinant cells?

Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?

Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?

What is the main purpose of genetic engineering?

What is the first stage in the genetic engineering process?

How does genetic engineering differ from classical breeding?

Which industries benefit from the application of genetic engineering?

What is the purpose of gel electrophoresis in the genetic engineering process?

What is a potential challenge faced by researchers in genetic engineering?

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