12 Questions
What is the purpose of using restriction endonucleases in the production of recombinant DNA?
To cleave the source DNA and plasmid or viral vectors at the same sites
What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?
To allow the plasmid DNA to enter the cells
What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?
To allow only 'transformed' cells to survive in the presence of the antibiotic
What is the purpose of the 'hybridization' step in the screening of recombinant cells?
To identify the cloned genes that form base-pairs with complementary sequences on another nucleic acid
Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?
Biolistics
Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?
Electroporation
What is the main purpose of genetic engineering?
To modify the traits of an organism by introducing new genes or altering gene expression
What is the first stage in the genetic engineering process?
Cleaving the source DNA using a restriction endonuclease
How does genetic engineering differ from classical breeding?
Classical breeding focuses on mating organisms with desirable traits, while genetic engineering modifies traits at the molecular level
Which industries benefit from the application of genetic engineering?
The pharmaceutical, industrial, agricultural, medical, and other industries
What is the purpose of gel electrophoresis in the genetic engineering process?
To separate DNA fragments based on their size
What is a potential challenge faced by researchers in genetic engineering?
Proving the significance of genetically modified organisms (GMOs) as a scientific breakthrough
Learn about how genetic engineering enables the creation of a wide range of plant-based products and fortified formulas by transferring DNA from one organism to another. Contrasting classical breeding with genetic engineering, this quiz explores the different techniques used to modify the traits of organisms.
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