Podcast
Questions and Answers
What is the purpose of using restriction endonucleases in the production of recombinant DNA?
What is the purpose of using restriction endonucleases in the production of recombinant DNA?
- To increase the pore sizes of the plasma membranes
- To identify the specific DNA fragment of interest
- To cleave the source DNA and plasmid or viral vectors at the same sites (correct)
- To join the DNA fragments to the plasmid or viral vector
What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?
What is the purpose of the 'heat shock' step in the plasmid insertion by heat shock treatment method?
- To concentrate the plasmids near the cells
- To increase the pore sizes of the plasma membranes
- To select for cells that have taken up the plasmid
- To allow the plasmid DNA to enter the cells (correct)
What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?
What is the purpose of including an antibiotic resistance gene in the plasmid DNA sequence?
- To allow only 'transformed' cells to survive in the presence of the antibiotic (correct)
- To increase the pore sizes of the plasma membranes
- To concentrate the plasmids near the cells
- To select for cells that have taken up the plasmid
What is the purpose of the 'hybridization' step in the screening of recombinant cells?
What is the purpose of the 'hybridization' step in the screening of recombinant cells?
Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?
Which of the following techniques uses a 'gene gun' to fire DNA-coated pellets on plant tissues?
Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?
Which of the following techniques uses electric shock to expand the membrane pores for the insertion of genes into mammalian cells?
What is the main purpose of genetic engineering?
What is the main purpose of genetic engineering?
What is the first stage in the genetic engineering process?
What is the first stage in the genetic engineering process?
How does genetic engineering differ from classical breeding?
How does genetic engineering differ from classical breeding?
Which industries benefit from the application of genetic engineering?
Which industries benefit from the application of genetic engineering?
What is the purpose of gel electrophoresis in the genetic engineering process?
What is the purpose of gel electrophoresis in the genetic engineering process?
What is a potential challenge faced by researchers in genetic engineering?
What is a potential challenge faced by researchers in genetic engineering?
