Podcast
Questions and Answers
What is the purpose of gene pharming in transgenic animals?
What is the purpose of gene pharming in transgenic animals?
- To produce larger animals for agricultural purposes
- To create animals with enhanced physical traits
- To produce pharmaceutical products for therapeutic and diagnostic uses (correct)
- To investigate the genetic makeup of endangered species
What is a significant challenge associated with animal cloning?
What is a significant challenge associated with animal cloning?
- Animal cloning has a high success rate
- Cloned animals have higher genetic diversity
- Most clones do not survive their early stages of life (correct)
- Cloning requires less research funding than traditional breeding
Which animal was the first successfully cloned organism?
Which animal was the first successfully cloned organism?
- A sheep known as 'Dolly' (correct)
- A pig named 'Sow'
- A cow named 'Luna'
- A goat known as 'Dolly'
How are bioengineered animals used in research?
How are bioengineered animals used in research?
What is one of the main applications of transgenic animals?
What is one of the main applications of transgenic animals?
What is the primary goal of recombinant DNA technology?
What is the primary goal of recombinant DNA technology?
What is the function of restriction enzymes in recombinant DNA technology?
What is the function of restriction enzymes in recombinant DNA technology?
What is the role of DNA ligase in recombinant DNA technology?
What is the role of DNA ligase in recombinant DNA technology?
Which of the following is NOT a component of the recombinant DNA process?
Which of the following is NOT a component of the recombinant DNA process?
What characteristic of plasmids makes them suitable vectors for genetic engineering?
What characteristic of plasmids makes them suitable vectors for genetic engineering?
How do sticky ends facilitate the process of recombinant DNA technology?
How do sticky ends facilitate the process of recombinant DNA technology?
What technique is used to produce millions of copies of a specific DNA sequence?
What technique is used to produce millions of copies of a specific DNA sequence?
What is a significant outcome of introducing foreign DNA into an organism?
What is a significant outcome of introducing foreign DNA into an organism?
What is the effect of BT GMOs on hornworms?
What is the effect of BT GMOs on hornworms?
What is glyphosate primarily used for in agriculture?
What is glyphosate primarily used for in agriculture?
What key nutrient deficiency does the production of Golden Rice aim to address?
What key nutrient deficiency does the production of Golden Rice aim to address?
What method was used to improve the nutritional value of Golden Rice?
What method was used to improve the nutritional value of Golden Rice?
Which of the following GM foods is noted for its improved taste?
Which of the following GM foods is noted for its improved taste?
What did genetic engineers achieve with crops resistant to glyphosate?
What did genetic engineers achieve with crops resistant to glyphosate?
What is one of the main safety concerns regarding GM foods?
What is one of the main safety concerns regarding GM foods?
What significant hurdle do herbicides like glyphosate present to crops?
What significant hurdle do herbicides like glyphosate present to crops?
What is one potential risk associated with Bt corn as mentioned?
What is one potential risk associated with Bt corn as mentioned?
What strategy do farmers employ to manage pest resistance when planting Bt crops?
What strategy do farmers employ to manage pest resistance when planting Bt crops?
What is a concern regarding gene flow from GM crops?
What is a concern regarding gene flow from GM crops?
What is the primary purpose of gene splicing?
What is the primary purpose of gene splicing?
What is terminator technology designed to prevent?
What is terminator technology designed to prevent?
How can transgenic organisms be formed according to the principles of molecular cloning?
How can transgenic organisms be formed according to the principles of molecular cloning?
What is a limitation of terminator technology?
What is a limitation of terminator technology?
What benefit does introducing the Bacillus thuringiensis (Bt) gene provide to cotton plants?
What benefit does introducing the Bacillus thuringiensis (Bt) gene provide to cotton plants?
Why are genetically modified plants developed that are resistant to drought?
Why are genetically modified plants developed that are resistant to drought?
How can a lack of genetic diversity impact agriculture?
How can a lack of genetic diversity impact agriculture?
What is the main concern about the interaction of GM crops with non-GM crops?
What is the main concern about the interaction of GM crops with non-GM crops?
Which of the following is NOT a characteristic of genetically modified plants mentioned?
Which of the following is NOT a characteristic of genetically modified plants mentioned?
What role do sticky ends play in molecular cloning?
What role do sticky ends play in molecular cloning?
Why is biodiversity important in agriculture?
Why is biodiversity important in agriculture?
What is one advantage of having plants that are resistant to insect pests?
What is one advantage of having plants that are resistant to insect pests?
What is the consequence of using 40% of chemical pesticides on cotton plants?
What is the consequence of using 40% of chemical pesticides on cotton plants?
Flashcards
Recombinant DNA Technology
Recombinant DNA Technology
A technique that combines DNA sequences from different sources to create a new DNA molecule.
Polymerase Chain Reaction (PCR)
Polymerase Chain Reaction (PCR)
The process of making multiple copies of a DNA fragment, often a gene.
Plasmid
Plasmid
A small, circular DNA molecule found in bacteria that can be used to carry and replicate genes.
Restriction Enzymes
Restriction Enzymes
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Sticky Ends
Sticky Ends
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DNA Ligase
DNA Ligase
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Transgenic Organisms
Transgenic Organisms
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Molecular Cloning
Molecular Cloning
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Gene splicing
Gene splicing
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Pest-resistant plants
Pest-resistant plants
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Bacillus thuringiensis (Bt)
Bacillus thuringiensis (Bt)
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Sticky ends in DNA
Sticky ends in DNA
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Drought-resistant plants
Drought-resistant plants
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Genetically modified fruits and vegetables
Genetically modified fruits and vegetables
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Genetically modified grains
Genetically modified grains
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Transgenic Animals
Transgenic Animals
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Gene Pharming
Gene Pharming
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Cloning
Cloning
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Bioengineered Animals in Research
Bioengineered Animals in Research
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Genetic Engineering for Personal Enhancement
Genetic Engineering for Personal Enhancement
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Harm to Non-Pest Organisms
Harm to Non-Pest Organisms
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Eliciting Pest Resistance
Eliciting Pest Resistance
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Gene Flow Disruption
Gene Flow Disruption
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Terminator Technology
Terminator Technology
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Environmental Impact
Environmental Impact
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Pest Resistance
Pest Resistance
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Genetic Diversity
Genetic Diversity
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Genetic Modification
Genetic Modification
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BT GMOs (e.g., Corn, Cotton)
BT GMOs (e.g., Corn, Cotton)
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Herbicide-Resistant Crops
Herbicide-Resistant Crops
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Genetically Modified Crops with Enhanced Nutrition
Genetically Modified Crops with Enhanced Nutrition
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Genetically Modified (GM) Foods
Genetically Modified (GM) Foods
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Flavr Savr Tomato
Flavr Savr Tomato
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Potential Risks of GM Foods
Potential Risks of GM Foods
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Potential Benefits of GM Foods
Potential Benefits of GM Foods
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Commercialization of GM Foods
Commercialization of GM Foods
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Study Notes
General Biology 2, Quarter 3, Week 1: Genetic Engineering
- This subject enhances understanding of biology principles, focusing on heredity, variation, organism diversity, structure, function, and evolution.
- Genetic engineering is the artificial manipulation, modification, and recombination of DNA molecules to modify organisms.
- Classical breeding involves mating or breeding selected organisms with desirable traits.
- Example: breeding fruit with bigger and sweeter fruit.
- Example: breeding dogs for specific traits.
- Recombinant DNA technology introduces new traits, enhances existing ones, or alters trait expression in an organism.
- Steps in classical breeding:
- Determine significant traits.
- Select exemplary parents.
- Choose best offspring.
- Repeat the process.
- Steps in creating recombinant DNA:
- Isolate plasmid DNA and DNA containing gene of interest.
- Insert gene into plasmid.
- Put plasmid into bacterial cell
- Cells cloned with desired gene
- Identify desired clone
- Get copies of gene and protein
- Steps in creating recombinant DNA (alternative):
- Cutting or cleaving DNA and plasmid by "restriction enzyme".
- Inserting gene of interest into the open plasmid by "DNA ligase".
- Insert recombinant plasmid back into bacterial cell.
- Isolate copies of the gene for insertion into other organisms.
- Examples of genetically modified plants:
- Bt corn: resistant to corn borer disease
- Bt eggplant: resistant to eggplant fruit and shoot borer.
- Golden rice: enhanced beta-carotene (Vitamin A precursor) content
- Other genetic engineering applications:
- Human insulin production
- Key concepts:
- DNA carries genetic information in all living things.
- RNA is a single-stranded nucleic acid involved in protein synthesis.
- Enzymes are protein molecules that catalyze biochemical reactions.
- Gene splicing: Inserting specific genes into another organism's genome, allowing the organism to express new traits.
- Plants with pest resistance: Genetically modified plants are made resistant to insects.
- Plants with herbicide resistance: Some plants are now resistant to herbicides, especially glyphosate.
- Plants with improved nutritional values: Golden rice is an example of a plant with improved vitamin A content.
- Genetically modified foods: Crop plants created using genetic engineering techniques for human or animal consumption.
- Potential risks of consuming GM foods: Potential unknown environmental hazards.
- Potential harm to other organisms: Monarch butterflies, example of potential indirect harm.
- Eliciting resistance: Developing resistance to insects and herbicides.
- Disrupting (or affecting) gene flow: The potential for introduced genes to spread, possibly reducing genetic diversity.
- Terminator technology: Seed sterilization to prevent unwanted genetic dispersal.
Additional Details
- Gene pharming: Producing pharmaceutical products in transgenic animals.
- Animal Cloning: Process involved in bioengineered animals.
- Steps for creating recombinant DNA (alternative 2):
- Isolate DNA from two sources
- Cut both DNAs by the “restriction enzyme”.
- Combine the DNAs and seal using "DNA ligase”.
- Transfer the recombinant DNA plasmid into the bacterial cell
- Isolation and insertion to other organisms to confer the desired trait
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