General Biology 2: Genetic Engineering Concepts

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Questions and Answers

What is the purpose of gene pharming in transgenic animals?

  • To produce larger animals for agricultural purposes
  • To create animals with enhanced physical traits
  • To produce pharmaceutical products for therapeutic and diagnostic uses (correct)
  • To investigate the genetic makeup of endangered species

What is a significant challenge associated with animal cloning?

  • Animal cloning has a high success rate
  • Cloned animals have higher genetic diversity
  • Most clones do not survive their early stages of life (correct)
  • Cloning requires less research funding than traditional breeding

Which animal was the first successfully cloned organism?

  • A sheep known as 'Dolly' (correct)
  • A pig named 'Sow'
  • A cow named 'Luna'
  • A goat known as 'Dolly'

How are bioengineered animals used in research?

<p>To study the genetics of human diseases (B)</p> Signup and view all the answers

What is one of the main applications of transgenic animals?

<p>Generating pharmaceutical products (D)</p> Signup and view all the answers

What is the primary goal of recombinant DNA technology?

<p>To clone a specific gene for analysis or protein production (B)</p> Signup and view all the answers

What is the function of restriction enzymes in recombinant DNA technology?

<p>To cut DNA molecules at specific regions (B)</p> Signup and view all the answers

What is the role of DNA ligase in recombinant DNA technology?

<p>To form strong bonds between DNA fragments (C)</p> Signup and view all the answers

Which of the following is NOT a component of the recombinant DNA process?

<p>Synthetic mRNA (A)</p> Signup and view all the answers

What characteristic of plasmids makes them suitable vectors for genetic engineering?

<p>They can replicate independently inside bacterial cells (A)</p> Signup and view all the answers

How do sticky ends facilitate the process of recombinant DNA technology?

<p>They allow complementary DNA fragments to bind together (D)</p> Signup and view all the answers

What technique is used to produce millions of copies of a specific DNA sequence?

<p>Polymerase chain reaction (PCR) (B)</p> Signup and view all the answers

What is a significant outcome of introducing foreign DNA into an organism?

<p>The organism may become transgenic (D)</p> Signup and view all the answers

What is the effect of BT GMOs on hornworms?

<p>They are toxic to hornworms. (C)</p> Signup and view all the answers

What is glyphosate primarily used for in agriculture?

<p>Killing actively growing plants and weeds. (B)</p> Signup and view all the answers

What key nutrient deficiency does the production of Golden Rice aim to address?

<p>Vitamin A deficiency. (B)</p> Signup and view all the answers

What method was used to improve the nutritional value of Golden Rice?

<p>Isolating genes from a daffodil flower. (D)</p> Signup and view all the answers

Which of the following GM foods is noted for its improved taste?

<p>Flavr Savr Tomato. (C)</p> Signup and view all the answers

What did genetic engineers achieve with crops resistant to glyphosate?

<p>They allowed crops to thrive despite herbicide application. (D)</p> Signup and view all the answers

What is one of the main safety concerns regarding GM foods?

<p>They might cause allergic reactions in humans. (A)</p> Signup and view all the answers

What significant hurdle do herbicides like glyphosate present to crops?

<p>They can stop crops from producing essential amino acids. (A)</p> Signup and view all the answers

What is one potential risk associated with Bt corn as mentioned?

<p>Potential harm to Monarch butterfly larvae (D)</p> Signup and view all the answers

What strategy do farmers employ to manage pest resistance when planting Bt crops?

<p>Planting a mixture of 20% non-Bt crops with Bt crops (B)</p> Signup and view all the answers

What is a concern regarding gene flow from GM crops?

<p>Pollen transfer leading to hybridization (D)</p> Signup and view all the answers

What is the primary purpose of gene splicing?

<p>To insert specific genes into the genome of a different organism (A)</p> Signup and view all the answers

What is terminator technology designed to prevent?

<p>Genetic contamination from GM crops (C)</p> Signup and view all the answers

How can transgenic organisms be formed according to the principles of molecular cloning?

<p>By combining any two unrelated DNA molecules with complementary sticky ends (B)</p> Signup and view all the answers

What is a limitation of terminator technology?

<p>It is not 100% effective in rendering GM crops sterile. (B)</p> Signup and view all the answers

What benefit does introducing the Bacillus thuringiensis (Bt) gene provide to cotton plants?

<p>It allows cotton to produce proteins toxic to specific insect pests (B)</p> Signup and view all the answers

Why are genetically modified plants developed that are resistant to drought?

<p>To ensure survival in changing environmental conditions (A)</p> Signup and view all the answers

How can a lack of genetic diversity impact agriculture?

<p>It may contribute to agricultural problems. (C)</p> Signup and view all the answers

What is the main concern about the interaction of GM crops with non-GM crops?

<p>Potential cross-pollination resulting in unwanted traits (B)</p> Signup and view all the answers

Which of the following is NOT a characteristic of genetically modified plants mentioned?

<p>Resistance to diseases caused by viruses (C)</p> Signup and view all the answers

What role do sticky ends play in molecular cloning?

<p>They facilitate the binding of DNA fragments from different sources (B)</p> Signup and view all the answers

Why is biodiversity important in agriculture?

<p>It enhances the stability and resilience of ecosystems. (B)</p> Signup and view all the answers

What is one advantage of having plants that are resistant to insect pests?

<p>They reduce the dependency on chemical pesticides (A)</p> Signup and view all the answers

What is the consequence of using 40% of chemical pesticides on cotton plants?

<p>It leads to environmental pollution and health risks (B)</p> Signup and view all the answers

Flashcards

Recombinant DNA Technology

A technique that combines DNA sequences from different sources to create a new DNA molecule.

Polymerase Chain Reaction (PCR)

The process of making multiple copies of a DNA fragment, often a gene.

Plasmid

A small, circular DNA molecule found in bacteria that can be used to carry and replicate genes.

Restriction Enzymes

Enzymes that cut DNA molecules at specific sequences, creating 'sticky ends' for joining.

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Sticky Ends

Short, single-stranded sequences of DNA that can bind to complementary sequences.

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DNA Ligase

An enzyme that joins two DNA fragments together, forming a strong bond between them.

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Transgenic Organisms

Organisms that contain DNA from another species.

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Molecular Cloning

The process of inserting a gene into a vector for replication and expression.

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Gene splicing

A process where specific gene sequences are inserted into the genome of another organism.

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Pest-resistant plants

Plants that have been genetically modified to resist pests without relying on pesticides.

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Bacillus thuringiensis (Bt)

A bacterium that produces a protein toxic to crop pests, used to create pest-resistant plants.

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Sticky ends in DNA

The ability of DNA with complementary sticky ends to join together, even if they are from different organisms.

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Drought-resistant plants

A type of genetic engineering that modifies plants to make them more resistant to drought.

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Genetically modified fruits and vegetables

Plants that have been genetically engineered to improve their taste, texture, size, or color.

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Genetically modified grains

Plants that have been genetically modified to have higher protein content.

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Transgenic Animals

A technique that involves introducing genes into animal eggs to produce offspring with desired traits, like larger size or enhanced characteristics.

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Gene Pharming

The process of using transgenic animals to manufacture pharmaceutical products for therapeutic and diagnostic purposes.

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Cloning

The creation of genetically identical copies of an organism, often used in research and potentially for therapeutic purposes.

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Bioengineered Animals in Research

The use of genetically engineered animals to study gene functions and their impact on human health.

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Genetic Engineering for Personal Enhancement

The ability to alter one's genetic makeup to achieve a 'perfect' version of oneself, potentially encompassing desired traits and eliminating undesirable traits.

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Harm to Non-Pest Organisms

The potential for Bt crops to negatively impact non-pest insects, like Monarch butterflies, causing biodiversity loss.

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Eliciting Pest Resistance

Bt crops can lead to the development of pest resistance, making them less effective over time. To mitigate this, farmers must plant non-Bt crops alongside Bt crops to slow down resistance.

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Gene Flow Disruption

The possibility of GM genes transferring from crops to wild relatives through pollen, potentially disrupting natural ecosystems and genetic diversity.

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Terminator Technology

Genetically modified crops that produce sterile seeds, designed to prevent unwanted genetic contamination in nature.

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Environmental Impact

The potential for GM crops to harm other organisms, including non-target insects like Monarch butterflies, and to disrupt gene flow in natural ecosystems.

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Pest Resistance

The process by which pests evolve resistance to pesticides or insecticides, becoming less susceptible to their effects.

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Genetic Diversity

The variety of different genes within a population of organisms, contributing to their ability to adapt to changing environments.

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Genetic Modification

The intentional introduction of genes from one organism to another, often for specific traits like pest resistance or herbicide tolerance.

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BT GMOs (e.g., Corn, Cotton)

Genetically modified organisms (GMOs) which produce toxins that harm specific pests, such as the BT toxin. This toxin is particularly effective against hornworms, a major pest of tomato and cotton.

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Herbicide-Resistant Crops

Plants that are genetically modified to resist herbicides, particularly glyphosate. These crops are not negatively impacted by glyphosate, which kills unwanted weeds.

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Genetically Modified Crops with Enhanced Nutrition

Genetic engineering has enabled scientists to modify crops, like Golden Rice, to increase their nutritional value and combat deficiencies.

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Genetically Modified (GM) Foods

Genetically modified foods are created using modern techniques like genetic engineering. This allows for changes in taste, size, nutritional value, and pest resistance.

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Flavr Savr Tomato

The Flavr Savr Tomato, a classic example of GM food, was engineered for improved flavor and longer shelf life.

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Potential Risks of GM Foods

While there are concerns about potential environmental risks associated with GM foods, current research indicates that those produced in the US do not contain allergens.

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Potential Benefits of GM Foods

GM foods offer potential benefits, such as increased nutritional value, pesticide resistance, and improved crop yield.

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Commercialization of GM Foods

There are over 40 plant varieties approved by the US Department of Agriculture for commercialization through genetic modification.

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Study Notes

General Biology 2, Quarter 3, Week 1: Genetic Engineering

  • This subject enhances understanding of biology principles, focusing on heredity, variation, organism diversity, structure, function, and evolution.
  • Genetic engineering is the artificial manipulation, modification, and recombination of DNA molecules to modify organisms.
  • Classical breeding involves mating or breeding selected organisms with desirable traits.
    • Example: breeding fruit with bigger and sweeter fruit.
    • Example: breeding dogs for specific traits.
  • Recombinant DNA technology introduces new traits, enhances existing ones, or alters trait expression in an organism.
  • Steps in classical breeding:
    • Determine significant traits.
    • Select exemplary parents.
    • Choose best offspring.
    • Repeat the process.
  • Steps in creating recombinant DNA:
    • Isolate plasmid DNA and DNA containing gene of interest.
    • Insert gene into plasmid.
    • Put plasmid into bacterial cell
    • Cells cloned with desired gene
    • Identify desired clone
    • Get copies of gene and protein
  • Steps in creating recombinant DNA (alternative):
    • Cutting or cleaving DNA and plasmid by "restriction enzyme".
    • Inserting gene of interest into the open plasmid by "DNA ligase".
    • Insert recombinant plasmid back into bacterial cell.
    • Isolate copies of the gene for insertion into other organisms.
  • Examples of genetically modified plants:
    • Bt corn: resistant to corn borer disease
    • Bt eggplant: resistant to eggplant fruit and shoot borer.
    • Golden rice: enhanced beta-carotene (Vitamin A precursor) content
  • Other genetic engineering applications:
    • Human insulin production
  • Key concepts:
    • DNA carries genetic information in all living things.
    • RNA is a single-stranded nucleic acid involved in protein synthesis.
    • Enzymes are protein molecules that catalyze biochemical reactions.
  • Gene splicing: Inserting specific genes into another organism's genome, allowing the organism to express new traits.
  • Plants with pest resistance: Genetically modified plants are made resistant to insects.
  • Plants with herbicide resistance: Some plants are now resistant to herbicides, especially glyphosate.
  • Plants with improved nutritional values: Golden rice is an example of a plant with improved vitamin A content.
  • Genetically modified foods: Crop plants created using genetic engineering techniques for human or animal consumption.
  • Potential risks of consuming GM foods: Potential unknown environmental hazards.
  • Potential harm to other organisms: Monarch butterflies, example of potential indirect harm.
  • Eliciting resistance: Developing resistance to insects and herbicides.
  • Disrupting (or affecting) gene flow: The potential for introduced genes to spread, possibly reducing genetic diversity.
  • Terminator technology: Seed sterilization to prevent unwanted genetic dispersal.

Additional Details

  • Gene pharming: Producing pharmaceutical products in transgenic animals.
  • Animal Cloning: Process involved in bioengineered animals.
  • Steps for creating recombinant DNA (alternative 2):
    • Isolate DNA from two sources
    • Cut both DNAs by the “restriction enzyme”.
    • Combine the DNAs and seal using "DNA ligase”.
    • Transfer the recombinant DNA plasmid into the bacterial cell
    • Isolation and insertion to other organisms to confer the desired trait

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