General Biology 1: Scanning Probe Microscope

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Questions and Answers

Who invented the first compound microscope?

  • Robert Hooke
  • Zacharias Janssen (correct)
  • King Charles II
  • Antonie Van Leeuwenhoek

Robert Hooke published his findings in a book called 'MICROGRAPHIA'.

True (A)

What term did Robert Hooke use to describe the small compartments he observed in cork?

cellula

Match the parts of the microscope with their functions:

<p>Eyepiece = Lens at the top of a microscope that the viewer looks through. Objective Lenses = Collects light passing through the specimen and focuses the light beam. Base = Supports the microscope. Nosepiece = Houses the objective lenses and allows for switching between them.</p> Signup and view all the answers

Which type of microscope is most commonly used in biology?

<p>Light Microscope (B)</p> Signup and view all the answers

The first scientists to observe sperm and egg cells were _____ and _____ cells.

<p>sperm, egg</p> Signup and view all the answers

What is the role of the diopter adjustment in a microscope?

<p>Corrects minor near- and far-sightedness.</p> Signup and view all the answers

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Study Notes

The Invention of Microscope

  • King Charles II of England collaborated with Robert Hooke to study insect anatomy.
  • Romans pioneered lens experimentation, leading to popular eyeglasses in the 13th century with magnification of 6x-10x.
  • The term "lens" comes from the Latin "lentil," reflecting the shape of lentil beans.
  • Zacharias Janssen invented the first compound microscope, enhancing magnification through multiple lenses.
  • Robert Hooke introduced the term "cellula" after examining cork, documenting his findings in 'MICROGRAPHIA'.
  • Antonie Van Leeuwenhoek developed a microscope with greater magnification, discovering bacteria, blood cells, and sperm.

Parts of the Microscope

  • Eyepiece (ocular lens): Standard magnification of 10x; interchangeable for 5x-30x.
  • Eyepiece Tube: Holds the eyepiece in alignment with objective lenses.
  • Diopter Adjustment: Corrects minor vision issues for the viewer.
  • Interpupillary Adjustment: Adjusts the distance between ocular lenses for user comfort.
  • Head: Connects eyepiece to objective lenses.
  • Arm: Connects head to base for structural support.
  • Base: Provides stability for the microscope.
  • Objective Lenses: Critical for magnifying specimens; includes:
    • Scanning Objective Lens (4x): For slide overview.
    • Lower Power Objective Lens (10x): General viewing.
    • High Power Objective Lens (40x): For fine detail observation.
    • Oil Immersion Objective Lens (100x): Highest power; requires immersion oil for clarity.

Types of Microscopes

  • Simple Microscope: Consists of a single lens.
  • Compound Microscope: Most common, utilizes multiple lenses for enhanced magnification.
  • Light Microscope: Primarily used in biology for illumination through light.
  • Electron Microscope: Uses electron beams for high-resolution imaging.
  • Stereomicroscope/Dissecting Microscope: Provides a 3D view for analyzing specimens.

Key Scientific Contributions

  • Robert Remak in 1855 established the concept that all cells arise from preexisting cells (Omnis cellula e cellula) by demonstrating cell division.

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