Histopathology
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Questions and Answers

What are the fixatives good for nucleic acid?

Carnoy and acetic alcohol

At which temperature formaline can fix DNA ?

  • 45
  • 50
  • 65 (correct)
  • 90
  • How formalin can fix the nucleic acid ?

    By fixing the nuclear protein

    The additive fixative can preserve the secondary structure of proteins

    <p>False</p> Signup and view all the answers

    Why don’t we use formalin for fixing the lipids

    <p>Because formalin can make the lipids soluble so the can be easily lost during the next process</p> Signup and view all the answers

    Formalin can dissolve lipids

    <p>False</p> Signup and view all the answers

    Which of the following can fix lipids

    <p>Gluteraldehyde</p> Signup and view all the answers

    How carbohydrates can be fixed ?

    <p>By entrapping them during protein fixation like glycogen</p> Signup and view all the answers

    Fixative colour may change the staining colour which make it difficult to diagnose

    <p>True</p> Signup and view all the answers

    What are the type of fixatives !

    <p>Immersion , perfusion, vapour, freezing , microwave , coating</p> Signup and view all the answers

    Perfusion disadvantage

    <p>Subjects die and highly cost with large volumes</p> Signup and view all the answers

    How would the temperature affect fixation

    <p>As the temperature increases the fixation rate will increase</p> Signup and view all the answers

    Prolonged fixation is better than under fixation

    <p>True</p> Signup and view all the answers

    The delay in fixation cause

    <p>Post Mortum changes</p> Signup and view all the answers

    What is the optimal time for 2mm section

    <p>8-12 hr</p> Signup and view all the answers

    For glycogen fixation formalin is enough

    <p>False</p> Signup and view all the answers

    The penetration rate of the fixative doesn’t depend on fixative concentration but the heat ?

    <p>True</p> Signup and view all the answers

    Tolerant fixative can cause antigen mask if exposed prolonged to tissue

    <p>True</p> Signup and view all the answers

    Which one is better 10%NBF or block cassettes for tissue storage !

    <p>It depends on the situation because NBF is good to preserve the whole tissue for long time but it can cause hardening and mask the antigen , block cassettes are goof for examinations of small tissue and it doesn’t cause antigen mask</p> Signup and view all the answers

    Prolonged fixation can introduce formalin pigment

    <p>True</p> Signup and view all the answers

    What is the characteristic of formalin

    <p>High penetration</p> Signup and view all the answers

    Which of the following formaline fixative is isotonic

    <p>10% Formailn saline</p> Signup and view all the answers

    Which of the following is good for lipid fixation

    <p>Calcium formalin</p> Signup and view all the answers

    Which of the following is good for glycogen fixation

    <p>Alcoholic formaline</p> Signup and view all the answers

    Which of the fixative good for neurons examination

    <p>Formalin ammonium bromide</p> Signup and view all the answers

    Tolerance material mask antigen without harm

    <p>True</p> Signup and view all the answers

    DNA is good for dna extraction

    <p>False</p> Signup and view all the answers

    Solid formaldehyde is called paraformaldehyde

    <p>True</p> Signup and view all the answers

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