Histopathology
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Questions and Answers

What are the fixatives good for nucleic acid?

Carnoy and acetic alcohol

At which temperature formaline can fix DNA ?

  • 45
  • 50
  • 65 (correct)
  • 90

How formalin can fix the nucleic acid ?

By fixing the nuclear protein

The additive fixative can preserve the secondary structure of proteins

<p>False (B)</p> Signup and view all the answers

Why don’t we use formalin for fixing the lipids

<p>Because formalin can make the lipids soluble so the can be easily lost during the next process</p> Signup and view all the answers

Formalin can dissolve lipids

<p>False (B)</p> Signup and view all the answers

Which of the following can fix lipids

<p>Gluteraldehyde (C), Osmium tetroxide (D)</p> Signup and view all the answers

How carbohydrates can be fixed ?

<p>By entrapping them during protein fixation like glycogen</p> Signup and view all the answers

Fixative colour may change the staining colour which make it difficult to diagnose

<p>True (A)</p> Signup and view all the answers

What are the type of fixatives !

<p>Immersion , perfusion, vapour, freezing , microwave , coating</p> Signup and view all the answers

Perfusion disadvantage

<p>Subjects die and highly cost with large volumes</p> Signup and view all the answers

How would the temperature affect fixation

<p>As the temperature increases the fixation rate will increase</p> Signup and view all the answers

Prolonged fixation is better than under fixation

<p>True (A)</p> Signup and view all the answers

The delay in fixation cause

<p>Post Mortum changes</p> Signup and view all the answers

What is the optimal time for 2mm section

<p>8-12 hr</p> Signup and view all the answers

For glycogen fixation formalin is enough

<p>False (B)</p> Signup and view all the answers

The penetration rate of the fixative doesn’t depend on fixative concentration but the heat ?

<p>True (A)</p> Signup and view all the answers

Tolerant fixative can cause antigen mask if exposed prolonged to tissue

<p>True (A)</p> Signup and view all the answers

Which one is better 10%NBF or block cassettes for tissue storage !

<p>It depends on the situation because NBF is good to preserve the whole tissue for long time but it can cause hardening and mask the antigen , block cassettes are goof for examinations of small tissue and it doesn’t cause antigen mask</p> Signup and view all the answers

Prolonged fixation can introduce formalin pigment

<p>True (A)</p> Signup and view all the answers

What is the characteristic of formalin

<p>High penetration (A), Slow cross links formation (B)</p> Signup and view all the answers

Which of the following formaline fixative is isotonic

<p>10% Formailn saline (B)</p> Signup and view all the answers

Which of the following is good for lipid fixation

<p>Calcium formalin (C)</p> Signup and view all the answers

Which of the following is good for glycogen fixation

<p>Alcoholic formaline (A)</p> Signup and view all the answers

Which of the fixative good for neurons examination

<p>Formalin ammonium bromide (D)</p> Signup and view all the answers

Tolerance material mask antigen without harm

<p>True (A)</p> Signup and view all the answers

DNA is good for dna extraction

<p>False (B)</p> Signup and view all the answers

Solid formaldehyde is called paraformaldehyde

<p>True (A)</p> Signup and view all the answers

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