15 Questions
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
Amplification
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
Primer
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
Probe
What is the target sequence/region to be amplified called?
Template
Which type of amplification involves making many copies of a specific DNA sequence?
Target Amplification
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
Amplification
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
Primer
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
Probe
What is the target sequence/region to be amplified called?
Template
Which method is the first and prototypical method for amplifying target nucleic acid?
PCR
Which probe amplification procedure involves the amplification of probe sequences using Ligase Chain Reaction (LCR)?
Ligase Chain Reaction (LCR)
Which signal amplification procedure is inherently better at quantifying the amount of target sequences present in the clinical sample?
Branch DNA (bDNA)
Which method was prototypical for amplifying target nucleic acid and was visualized by Kary Mullis in 1983?
Polymerase Chain Reaction (PCR)
Which probe amplification procedure involves the amplification of probe sequences using Strand Displacement Amplification (SDA)?
Strand Displacement Amplification (SDA)
Which signal amplification procedure involves the binding of large amounts of a signal to the target sequences in the sample?
Hybrid Capture Assay
Study Notes
Nucleic Acid Amplification
- PCR (Polymerase Chain Reaction) is a process that involves increasing the number of nucleic acid copies in a sample to millions within a short period.
Primer Synthesis
- A short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region is called a primer.
Probes
- A nucleic acid with a detectable signal that specifically binds to a complementary sequence is called a probe.
Target Amplification
- The target sequence/region to be amplified is the specific DNA sequence being amplified.
Amplification Methods
- PCR (Polymerase Chain Reaction) is a type of amplification that involves making many copies of a specific DNA sequence.
- PCR is the first and prototypical method for amplifying target nucleic acid.
- Other probe amplification procedures include Ligase Chain Reaction (LCR) and Strand Displacement Amplification (SDA).
- PCR was visualized by Kary Mullis in 1983.
Signal Amplification
- Signal amplification procedures include:
- Ligase Chain Reaction (LCR)
- Strand Displacement Amplification (SDA)
- Binding of large amounts of a signal to the target sequences in the sample (inherently better at quantifying the amount of target sequences present in the clinical sample)
Test your knowledge on terms related to nucleic acid amplification, including primer, probe, DNA template, and fluorophore. Learn about the process of increasing nucleic acid copies and the use of specific DNA fragments.
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