Podcast
Questions and Answers
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
- Amplification (correct)
- Probe binding
- Fluorescence emission
- Primer synthesis
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
- Template
- Primer (correct)
- Amplification
- Probe
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
- Template
- Probe (correct)
- Primer
- Amplification
What is the target sequence/region to be amplified called?
What is the target sequence/region to be amplified called?
Which type of amplification involves making many copies of a specific DNA sequence?
Which type of amplification involves making many copies of a specific DNA sequence?
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
Which process involves increasing the number of nucleic acid copies in a sample to millions within a short period?
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
What is a short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region called?
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
What is a nucleic acid with a detectable signal that specifically binds to a complementary sequence called?
What is the target sequence/region to be amplified called?
What is the target sequence/region to be amplified called?
Which method is the first and prototypical method for amplifying target nucleic acid?
Which method is the first and prototypical method for amplifying target nucleic acid?
Which probe amplification procedure involves the amplification of probe sequences using Ligase Chain Reaction (LCR)?
Which probe amplification procedure involves the amplification of probe sequences using Ligase Chain Reaction (LCR)?
Which signal amplification procedure is inherently better at quantifying the amount of target sequences present in the clinical sample?
Which signal amplification procedure is inherently better at quantifying the amount of target sequences present in the clinical sample?
Which method was prototypical for amplifying target nucleic acid and was visualized by Kary Mullis in 1983?
Which method was prototypical for amplifying target nucleic acid and was visualized by Kary Mullis in 1983?
Which probe amplification procedure involves the amplification of probe sequences using Strand Displacement Amplification (SDA)?
Which probe amplification procedure involves the amplification of probe sequences using Strand Displacement Amplification (SDA)?
Which signal amplification procedure involves the binding of large amounts of a signal to the target sequences in the sample?
Which signal amplification procedure involves the binding of large amounts of a signal to the target sequences in the sample?
Study Notes
Nucleic Acid Amplification
- PCR (Polymerase Chain Reaction) is a process that involves increasing the number of nucleic acid copies in a sample to millions within a short period.
Primer Synthesis
- A short, single-stranded DNA fragment designed for primer synthesis of a specific DNA region is called a primer.
Probes
- A nucleic acid with a detectable signal that specifically binds to a complementary sequence is called a probe.
Target Amplification
- The target sequence/region to be amplified is the specific DNA sequence being amplified.
Amplification Methods
- PCR (Polymerase Chain Reaction) is a type of amplification that involves making many copies of a specific DNA sequence.
- PCR is the first and prototypical method for amplifying target nucleic acid.
- Other probe amplification procedures include Ligase Chain Reaction (LCR) and Strand Displacement Amplification (SDA).
- PCR was visualized by Kary Mullis in 1983.
Signal Amplification
- Signal amplification procedures include:
- Ligase Chain Reaction (LCR)
- Strand Displacement Amplification (SDA)
- Binding of large amounts of a signal to the target sequences in the sample (inherently better at quantifying the amount of target sequences present in the clinical sample)
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Description
Test your knowledge on terms related to nucleic acid amplification, including primer, probe, DNA template, and fluorophore. Learn about the process of increasing nucleic acid copies and the use of specific DNA fragments.
