EMBRYOLOGY-3

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Questions and Answers

What role does hyaluronidase play during the fertilization process?

  • It breaks down the sticky matrix of the corona radiata. (correct)
  • It facilitates the fusion of the sperm with the oocyte.
  • It increases the calcium concentration in the sperm.
  • It assists in the penetration of the zona pellucida.

During which phase of fertilization does the sperm attach to and penetrate the zona pellucida?

  • Pre-fertilization phase
  • Phase 2 (correct)
  • Phase 3
  • Phase 1

Which component of the spermatozoon aids its attachment to the oocyte's plasma membrane?

  • Zonal proteins
  • Antigens on its head (correct)
  • Acrosin enzymes
  • Granulosa cells

What is the result of spermatozoa penetrating the zona pellucida?

<p>Only one spermatozoon attaches to the plasma membrane of the oocyte. (B)</p> Signup and view all the answers

Which phase of fertilization involves the spermatozoa swimming through the granulosa cells?

<p>Phase 1 (D)</p> Signup and view all the answers

What type of proteins do spermatozoa bind to when they reach the zona pellucida?

<p>ZP3 proteins (C)</p> Signup and view all the answers

What happens to the components of the spermatozoon during fusion with the oocyte's plasma membrane?

<p>The head, midpiece, and tail sink into the cytoplasm. (A)</p> Signup and view all the answers

Why is the ampullary region of the Fallopian tube significant for fertilization?

<p>It provides an optimal environment for sperm and oocyte interaction. (B)</p> Signup and view all the answers

What is the primary function of the syncytiotrophoblast during implantation?

<p>To erode the endometrium and create a passage for the blastocyst (A)</p> Signup and view all the answers

What role does hCG play in early pregnancy maintenance?

<p>It maintains the functional activity of the corpus luteum (C)</p> Signup and view all the answers

What is a function of decidual cells in the endometrium?

<p>They produce substances that can either support or inhibit implantation (B)</p> Signup and view all the answers

What does the term 'decidual reaction' refer to?

<p>Changes in the lamina propria of the endometrium (A)</p> Signup and view all the answers

Which of the following best describes the cytotrophoblast?

<p>It is the internal part of the trophoblast (B)</p> Signup and view all the answers

What impact does progesterone have during early pregnancy?

<p>It regulates endometrial functions and trophoblast activity (B)</p> Signup and view all the answers

What process occurs when the syncytiotrophoblast contacts maternal blood vessels?

<p>Establishment of nutrient supply through lacunae (C)</p> Signup and view all the answers

During implantation, which structure is primarily responsible for providing nutrients to the embryo?

<p>Lacunae filled with maternal blood (B)</p> Signup and view all the answers

What major change occurs in the male nucleus during the formation of the male pronucleus?

<p>Decondensation of genetic material (C)</p> Signup and view all the answers

Which of the following is NOT a role of the zona pellucida?

<p>Facilitates differentiation of inner blastomeres (C)</p> Signup and view all the answers

What is the outcome of fertilisation regarding the genetic composition of the zygote?

<p>Zygote is restored to a diploid number with 23 maternal and 23 paternal chromosomes (C)</p> Signup and view all the answers

Which stage follows cleavage in early embryonic development?

<p>Compaction (D)</p> Signup and view all the answers

How long after fertilisation does cleavage typically begin?

<p>30 hours (D)</p> Signup and view all the answers

What differentiates outer blastomeres from inner blastomeres during compaction?

<p>Outer blastomeres are in contact with the zona pellucida (C)</p> Signup and view all the answers

What triggers the acrosomal reaction during fertilisation?

<p>Contact with the zona pellucida (C)</p> Signup and view all the answers

What is the primary function of the blastomeres during cleavage?

<p>Increase the number of cells within the same cytoplasm (A)</p> Signup and view all the answers

What is the primary role of the trophoblast in early embryonic development?

<p>Creation of the placenta (B)</p> Signup and view all the answers

Which type of junctions primarily hold the outer blastomeres together?

<p>Tight junctions (A)</p> Signup and view all the answers

What initiates the formation of the blastocoele in the morula?

<p>Flow of fluids mediated by the zona pellucida (A)</p> Signup and view all the answers

What is the primary function of the Early Pregnancy Factor produced by the trophoblast?

<p>Immunosuppression to avoid maternal immune attack (D)</p> Signup and view all the answers

During which phase does the embryo begin to depend on its own genetic material?

<p>Blastula (D)</p> Signup and view all the answers

Which term describes the phenomenon where the subdivision of chromosomes during cleavage does not occur correctly?

<p>Mosaicism (C)</p> Signup and view all the answers

What is the significance of the polarized structure of the blastocyst?

<p>Establishment of embryonic and abembryonic poles (A)</p> Signup and view all the answers

Where does the cleavage stage of embryo development predominantly take place?

<p>Fallopian tube (D)</p> Signup and view all the answers

What role do JUNO receptors play during the process of fertilization?

<p>They are removed from the egg membrane to prevent additional sperm attachment. (C)</p> Signup and view all the answers

What is one way the paternal mitochondria are eliminated after fertilization?

<p>Through degradation by macrophages. (B)</p> Signup and view all the answers

What is the purpose of the fast block to polyspermy?

<p>To cause membrane depolarization and temporarily prevent further sperm adhesion. (B)</p> Signup and view all the answers

What triggers the permanent block to polyspermy?

<p>Release of JUNO and the secretion of contents from cortical granules. (C)</p> Signup and view all the answers

What is the significance of calcium changes during fertilization?

<p>They are involved in the activation of egg metabolism and prevention of polyspermy. (B)</p> Signup and view all the answers

What occurs to the oocyte once it is fertilized?

<p>It becomes a fertilized oocyte with two pronuclei. (B)</p> Signup and view all the answers

How many viable cells result from female meiosis?

<p>One viable cell and three polar bodies. (D)</p> Signup and view all the answers

What happens to mRNA after the fertilization process begins?

<p>It gets actively recruited to facilitate egg metabolism. (B)</p> Signup and view all the answers

What describes the relationship between the epiblast and the amniotic cavity?

<p>The epiblast is dorsal and forms the amniotic cavity. (A)</p> Signup and view all the answers

What does the hypoblast contribute to in early development?

<p>Development of the parietal endoderm (Heuser's membrane). (B)</p> Signup and view all the answers

Which statement is correct regarding the yolk sac?

<p>The yolk sac is beneficial in oviparous animals but not in humans. (A)</p> Signup and view all the answers

What structure surrounds the amniotic cavity as development progresses?

<p>Chorion. (D)</p> Signup and view all the answers

What role does the body stalk play in embryo development?

<p>It is the first site of blood vessel formation. (C)</p> Signup and view all the answers

What does the extraembryonic coelom develop into during embryonic development?

<p>Chorionic cavity. (B)</p> Signup and view all the answers

At what stage does the vitelline sac significantly decrease in size?

<p>By the 13th day of development. (D)</p> Signup and view all the answers

Which structure is primarily formed from the growth of hypoblast cells?

<p>Yolk sac. (B)</p> Signup and view all the answers

Flashcards

Fertilization

The process of a sperm cell fertilizing an egg cell.

Perivitelline Space

The space surrounding the egg cell, between the zona pellucida and the plasma membrane.

Zona Pellucida

A protein layer surrounding the egg cell, acting as a barrier to sperm.

Hyaluronidase

An enzyme released by the sperm's acrosome, aiding in the breakdown of the egg's outer layer.

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Acrosome

The sperm's head structure containing enzymes vital for fertilization.

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ZP3

A key protein on the zona pellucida, binding to specific sperm proteins, ensuring species compatibility.

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Fusion Phase

The process of the sperm cell fusing with the egg cell membrane and the head, mid piece and tail entering the cytoplasm.

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Polyspermy

The condition where multiple sperm cells penetrate the egg.

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JUNO Receptors

Specialized receptors on the egg membrane that bind to IZUMO antigens on the sperm head.

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IZUMO Antigens

Antigens located on the spermatozoa's head that bind to JUNO receptors on the egg membrane.

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Prevention of Polyspermy

The process of preventing multiple sperm from fertilizing the egg.

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Fast Block

A rapid electrical change in the egg's membrane potential, lasting only a few minutes, to prevent sperm attachment.

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Permanent Block

A long-lasting block to polyspermy involving changes in calcium levels, cortical granule release, and zona pellucida hardening.

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Ootide

A fertilized oocyte with two pronuclei (one from the sperm and one from the egg).

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Completion of Meiosis II

The process where the female nucleus completes meiosis II, resulting in a second polar body.

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Egg Activation

The activation of the egg's metabolic processes, including mRNA recruitment and increased respiration.

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Male pronucleus formation

The process where the sperm cell's genetic material (DNA) becomes less tightly packed, allowing for the formation of the male pronucleus.

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Zygote formation

The fusion of the male and female pronuclei, restoring the zygote to its normal diploid number of chromosomes (23 from each parent).

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Cleavage

The process of repeated cell division in the zygote, increasing the number of cells without increasing the overall size. This is like dividing a cake but keeping the total amount of cake the same.

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Outer blastomeres

The outer blastomeres (cells) in the morula, which will eventually contribute to the trophoblast, an essential part of the placenta.

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Inner blastomeres

The inner blastomeres (cells) in the morula, which will eventually give rise to the embryo itself, the baby you see!

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Compaction

The process by which blastomeres tightly connect to each other, increasing cell-cell contact. This is like a team of people holding hands tightly together.

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Morula

The stage of development characterized by the formation of 16 blastomeres, a solid ball of cells.

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Trophoblast

The outer layer of cells in a blastocyst that will contribute to the formation of the placenta.

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Inner Cell Mass

The inner group of cells in a blastocyst that will develop into the embryo itself.

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Blastocoele

The fluid-filled cavity that forms within the blastocyst, separating the inner cell mass from the trophoblast.

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Early Pregnancy Factor

A protein produced by the trophoblast that helps suppress the mother's immune system, preventing rejection of the embryo.

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Blastula Period

The stage in early embryonic development where the embryo starts relying on its own genetic information for growth.

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Mosaicism

A condition where cells within the early embryo have different genetic material due to errors in chromosome division during cleavage.

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Polar Trophoblast

The outer layer of the trophoblast facing the inner cell mass.

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Epiblast vs. Hypoblast

The epiblast is the dorsal layer of the bilaminar germ disc and develops into the amniotic cavity. The hypoblast is the ventral layer and develops into the yolk sac.

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Amniotic Cavity

The amniotic cavity forms within the epiblast and is filled with amniotic fluid, protecting the developing embryo.

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Yolk Sac

The yolk sac is a membrane-bound sac that develops from the hypoblast. It plays a role in transferring nutrients and gases to the embryo in some species.

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Parietal Endoderm

The parietal endoderm is a layer of cells that lines the yolk sac. It's formed by the proliferation of hypoblast cells.

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Extraembryonic Mesoderm

The extraembryonic mesoderm is a layer of tissue derived from the parietal endoderm. It supports the amniotic sac, yolk sac, and chorionic villi.

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Chorion

The chorion is the outer shell of the blastocyst, comprised of the extraembryonic mesoderm and the cytotrophoblast. It plays a role in the formation of the placenta.

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Extraembryonic Coelom

The extraembryonic coelom (or chorionic cavity) is a fluid-filled cavity that develops within the chorion, surrounding the amniotic sac and yolk sac.

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Body Stalk

The body stalk is a cord of tissue connecting the embryo to the chorion through the yolk sac and amniotic sac. It serves as the starting point for blood vessel formation.

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Syncytiotrophoblast

The outer layer of the trophoblast, highly invasive, erodes the endometrium creating a passage for the blastocyst, comes into contact with maternal blood vessels and is filled with maternal blood.

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Cytotrophoblast

The inner part of the trophoblast, less invasive, contributes to the formation of the placenta.

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Implantation

The process by which the trophoblast erodes the endometrium to allow the implantation of the blastocyst.

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Lacunae

The space within the syncytiotrophoblast filled with maternal blood, providing nutrients to the developing embryo.

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Progesterone

A hormone produced by the corpus luteum, vital for regulating endometrial functions and the proteolytic activity of the trophoblast.

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hCG (human Chorionic Gonadotropin)

A hormone produced by the trophoblast, maintains the functionality of the corpus luteum, detectable in maternal blood by day 8 and urine by day 10.

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Decidual reaction

The changes happening in the lamina propria of the endometrium, involving the secretion of interleukins, growth factors.

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Immuno-tolerance

The process by which the maternal immune system tolerates the presence of the embryo, a semi-foreign entity.

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Study Notes

Fertilization

  • The egg is significantly larger than the sperm cell, approximately 10 million times larger in volume.
  • Fertilization occurs in the ampullary region of the fallopian tube due to the folds which slow the oocyte. Optimal conditions are created for sperm to meet the oocyte.
  • Several changes need to occur for fertilization to take place.

Phase 1 of Fertilization

  • Sperm travel among the granulosa cells of the corona radiata.
  • Hyaluronidase is released, breaking down the hyaluronic acid, allowing the sperm to move.
  • Spermatozoa then rapidly swim to the zona pellucida.
  • Sometimes, enzymes from the uterine tube aid the process.

Phase 2 of Fertilization

  • Sperm reach the zona pellucida and attach.
  • Sperm bind to zona proteins (mainly ZP3), which are species-specific.
  • Acrosome enzymes (like acrosin) digest the membrane.
  • Calcium concentration increases, causing the sperm head to fuse with the zona pellucida.
  • Spermatozoa penetrate the zona pellucida, entering the perivitelline space.
  • Only one sperm will attach to the oocyte's plasma membrane.

Phase 3 of Fertilization

  • One sperm attaches to the oocyte's microvilli via antigens (IZUMO) on the sperm head that bind to receptors (JUNO) on the egg membrane.
  • The sperm head, midpiece, and tail enter the cytoplasm.
  • Mitochondria from the sperm remain outside.
  • Juno receptors are ejected to prevent polyspermy.

Phase 4 of Fertilization

  • Prevention of polyspermy:
    • Fast block: membrane depolarizes within seconds.
    • Permanent Block: Calcium waves trigger cortical granule release, causing swelling and hardening of the zona pellucida.

Phase 5 of Fertilization

  • The female nucleus completes meiosis II.
  • A second polar body is formed.
  • Two pronuclei fuse.

Cleavage

  • Cleavage increases cell number within the zona pellucida without increasing the size of the zygote.
  • Cleavage begins around 30 hours after fertilization.
  • Mammalian cleavage takes several days.
  • Cleavage leads to blastomeres and a morula, which forms a blastocyst in around 3 days.

Compaction

  • Blastomeres become tightly compacted, increasing cell-to-cell contact.

Blastocyst formation

  • Blastomeres move towards one side of the embryo.
  • A cavity called the blastocoele forms within the blastocyst.
  • The embryo is composed of an outer trophoblast layer and an inner cell mass (embryoblast), and the zona pellucida is still present.

Early Pregnancy Factor (EPF)

  • EPF is produced by the trophoblast.
  • It is believed to help avoid a mother's immune responses.

Blastocyst Polarity

  • Blastocysts have a polar side (the inner cell mass) and an opposite (mural), abembryonic side, where the trophoblast covers the surface..

Implantation

  • The blastocyst implants in a prepared uterine environment at the end of the previous menstrual cycle.
  • Three stages:
    • Apposition (approaches the endometrium)
    • Adhesion (close contact with adhesion molecules)
    • Invasion (trophoblasts erode the endometrium)

Zygote, embryonic cells and chromosomal issues

  • The zygote initially depends on maternal genetic material.
  • The embryo generates its own genetic material during blastula.
  • Mishap in chromosomal division during cleavage can lead to mosaicism.

Implantation Window

  • A specific timeframe when the endometrium is receptive to embryo implantation.
  • Implantation begins 4-6 days after the LH surge.

Invasive Trophoblast

  • Syncytiotrophoblast: erosive and invasive.
  • It digests endometrium for implantation passageway

Maintenance of Pregnancy

  • Corpus luteum: produces progesterone, regulating endometrial function and trophoblastic activity.
  • hCG (human chorionic gonadotropin): maintains the corpus luteum.
  • Decidual reaction in the endometrial stroma protects the embryo.

Ectopic Pregnancy

  • Implantation occurs outside of the uterine cavity.
  • Commonly in the uterine tubes.

Second Week

  • The embryo rearranges and forms an epiblast and hypoblast layers.
  • Amniotic and yolk sac development.

Implantation Sites

  • The typical implantation site is the uterine posterior wall.
  • This is not the only implantation site; other locations are possible, but they can lead to complications.

Pregnancy Loss (Miscarriage)

  • Spontaneous abortions frequently occur during the first 12 weeks of pregnancy.
  • Reasons for miscarriage are multiple, including chromosomal abnormalities or maternal and placental or fetal causes.

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