Podcast
Questions and Answers
What role does hyaluronidase play during the fertilization process?
What role does hyaluronidase play during the fertilization process?
- It breaks down the sticky matrix of the corona radiata. (correct)
- It facilitates the fusion of the sperm with the oocyte.
- It increases the calcium concentration in the sperm.
- It assists in the penetration of the zona pellucida.
During which phase of fertilization does the sperm attach to and penetrate the zona pellucida?
During which phase of fertilization does the sperm attach to and penetrate the zona pellucida?
- Pre-fertilization phase
- Phase 2 (correct)
- Phase 3
- Phase 1
Which component of the spermatozoon aids its attachment to the oocyte's plasma membrane?
Which component of the spermatozoon aids its attachment to the oocyte's plasma membrane?
- Zonal proteins
- Antigens on its head (correct)
- Acrosin enzymes
- Granulosa cells
What is the result of spermatozoa penetrating the zona pellucida?
What is the result of spermatozoa penetrating the zona pellucida?
Which phase of fertilization involves the spermatozoa swimming through the granulosa cells?
Which phase of fertilization involves the spermatozoa swimming through the granulosa cells?
What type of proteins do spermatozoa bind to when they reach the zona pellucida?
What type of proteins do spermatozoa bind to when they reach the zona pellucida?
What happens to the components of the spermatozoon during fusion with the oocyte's plasma membrane?
What happens to the components of the spermatozoon during fusion with the oocyte's plasma membrane?
Why is the ampullary region of the Fallopian tube significant for fertilization?
Why is the ampullary region of the Fallopian tube significant for fertilization?
What is the primary function of the syncytiotrophoblast during implantation?
What is the primary function of the syncytiotrophoblast during implantation?
What role does hCG play in early pregnancy maintenance?
What role does hCG play in early pregnancy maintenance?
What is a function of decidual cells in the endometrium?
What is a function of decidual cells in the endometrium?
What does the term 'decidual reaction' refer to?
What does the term 'decidual reaction' refer to?
Which of the following best describes the cytotrophoblast?
Which of the following best describes the cytotrophoblast?
What impact does progesterone have during early pregnancy?
What impact does progesterone have during early pregnancy?
What process occurs when the syncytiotrophoblast contacts maternal blood vessels?
What process occurs when the syncytiotrophoblast contacts maternal blood vessels?
During implantation, which structure is primarily responsible for providing nutrients to the embryo?
During implantation, which structure is primarily responsible for providing nutrients to the embryo?
What major change occurs in the male nucleus during the formation of the male pronucleus?
What major change occurs in the male nucleus during the formation of the male pronucleus?
Which of the following is NOT a role of the zona pellucida?
Which of the following is NOT a role of the zona pellucida?
What is the outcome of fertilisation regarding the genetic composition of the zygote?
What is the outcome of fertilisation regarding the genetic composition of the zygote?
Which stage follows cleavage in early embryonic development?
Which stage follows cleavage in early embryonic development?
How long after fertilisation does cleavage typically begin?
How long after fertilisation does cleavage typically begin?
What differentiates outer blastomeres from inner blastomeres during compaction?
What differentiates outer blastomeres from inner blastomeres during compaction?
What triggers the acrosomal reaction during fertilisation?
What triggers the acrosomal reaction during fertilisation?
What is the primary function of the blastomeres during cleavage?
What is the primary function of the blastomeres during cleavage?
What is the primary role of the trophoblast in early embryonic development?
What is the primary role of the trophoblast in early embryonic development?
Which type of junctions primarily hold the outer blastomeres together?
Which type of junctions primarily hold the outer blastomeres together?
What initiates the formation of the blastocoele in the morula?
What initiates the formation of the blastocoele in the morula?
What is the primary function of the Early Pregnancy Factor produced by the trophoblast?
What is the primary function of the Early Pregnancy Factor produced by the trophoblast?
During which phase does the embryo begin to depend on its own genetic material?
During which phase does the embryo begin to depend on its own genetic material?
Which term describes the phenomenon where the subdivision of chromosomes during cleavage does not occur correctly?
Which term describes the phenomenon where the subdivision of chromosomes during cleavage does not occur correctly?
What is the significance of the polarized structure of the blastocyst?
What is the significance of the polarized structure of the blastocyst?
Where does the cleavage stage of embryo development predominantly take place?
Where does the cleavage stage of embryo development predominantly take place?
What role do JUNO receptors play during the process of fertilization?
What role do JUNO receptors play during the process of fertilization?
What is one way the paternal mitochondria are eliminated after fertilization?
What is one way the paternal mitochondria are eliminated after fertilization?
What is the purpose of the fast block to polyspermy?
What is the purpose of the fast block to polyspermy?
What triggers the permanent block to polyspermy?
What triggers the permanent block to polyspermy?
What is the significance of calcium changes during fertilization?
What is the significance of calcium changes during fertilization?
What occurs to the oocyte once it is fertilized?
What occurs to the oocyte once it is fertilized?
How many viable cells result from female meiosis?
How many viable cells result from female meiosis?
What happens to mRNA after the fertilization process begins?
What happens to mRNA after the fertilization process begins?
What describes the relationship between the epiblast and the amniotic cavity?
What describes the relationship between the epiblast and the amniotic cavity?
What does the hypoblast contribute to in early development?
What does the hypoblast contribute to in early development?
Which statement is correct regarding the yolk sac?
Which statement is correct regarding the yolk sac?
What structure surrounds the amniotic cavity as development progresses?
What structure surrounds the amniotic cavity as development progresses?
What role does the body stalk play in embryo development?
What role does the body stalk play in embryo development?
What does the extraembryonic coelom develop into during embryonic development?
What does the extraembryonic coelom develop into during embryonic development?
At what stage does the vitelline sac significantly decrease in size?
At what stage does the vitelline sac significantly decrease in size?
Which structure is primarily formed from the growth of hypoblast cells?
Which structure is primarily formed from the growth of hypoblast cells?
Flashcards
Fertilization
Fertilization
The process of a sperm cell fertilizing an egg cell.
Perivitelline Space
Perivitelline Space
The space surrounding the egg cell, between the zona pellucida and the plasma membrane.
Zona Pellucida
Zona Pellucida
A protein layer surrounding the egg cell, acting as a barrier to sperm.
Hyaluronidase
Hyaluronidase
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Acrosome
Acrosome
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ZP3
ZP3
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Fusion Phase
Fusion Phase
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Polyspermy
Polyspermy
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JUNO Receptors
JUNO Receptors
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IZUMO Antigens
IZUMO Antigens
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Prevention of Polyspermy
Prevention of Polyspermy
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Fast Block
Fast Block
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Permanent Block
Permanent Block
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Ootide
Ootide
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Completion of Meiosis II
Completion of Meiosis II
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Egg Activation
Egg Activation
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Male pronucleus formation
Male pronucleus formation
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Zygote formation
Zygote formation
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Cleavage
Cleavage
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Outer blastomeres
Outer blastomeres
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Inner blastomeres
Inner blastomeres
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Compaction
Compaction
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Morula
Morula
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Trophoblast
Trophoblast
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Inner Cell Mass
Inner Cell Mass
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Blastocoele
Blastocoele
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Early Pregnancy Factor
Early Pregnancy Factor
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Blastula Period
Blastula Period
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Mosaicism
Mosaicism
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Polar Trophoblast
Polar Trophoblast
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Epiblast vs. Hypoblast
Epiblast vs. Hypoblast
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Amniotic Cavity
Amniotic Cavity
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Yolk Sac
Yolk Sac
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Parietal Endoderm
Parietal Endoderm
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Extraembryonic Mesoderm
Extraembryonic Mesoderm
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Chorion
Chorion
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Extraembryonic Coelom
Extraembryonic Coelom
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Body Stalk
Body Stalk
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Syncytiotrophoblast
Syncytiotrophoblast
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Cytotrophoblast
Cytotrophoblast
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Implantation
Implantation
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Lacunae
Lacunae
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Progesterone
Progesterone
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hCG (human Chorionic Gonadotropin)
hCG (human Chorionic Gonadotropin)
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Decidual reaction
Decidual reaction
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Immuno-tolerance
Immuno-tolerance
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Study Notes
Fertilization
- The egg is significantly larger than the sperm cell, approximately 10 million times larger in volume.
- Fertilization occurs in the ampullary region of the fallopian tube due to the folds which slow the oocyte. Optimal conditions are created for sperm to meet the oocyte.
- Several changes need to occur for fertilization to take place.
Phase 1 of Fertilization
- Sperm travel among the granulosa cells of the corona radiata.
- Hyaluronidase is released, breaking down the hyaluronic acid, allowing the sperm to move.
- Spermatozoa then rapidly swim to the zona pellucida.
- Sometimes, enzymes from the uterine tube aid the process.
Phase 2 of Fertilization
- Sperm reach the zona pellucida and attach.
- Sperm bind to zona proteins (mainly ZP3), which are species-specific.
- Acrosome enzymes (like acrosin) digest the membrane.
- Calcium concentration increases, causing the sperm head to fuse with the zona pellucida.
- Spermatozoa penetrate the zona pellucida, entering the perivitelline space.
- Only one sperm will attach to the oocyte's plasma membrane.
Phase 3 of Fertilization
- One sperm attaches to the oocyte's microvilli via antigens (IZUMO) on the sperm head that bind to receptors (JUNO) on the egg membrane.
- The sperm head, midpiece, and tail enter the cytoplasm.
- Mitochondria from the sperm remain outside.
- Juno receptors are ejected to prevent polyspermy.
Phase 4 of Fertilization
- Prevention of polyspermy:
- Fast block: membrane depolarizes within seconds.
- Permanent Block: Calcium waves trigger cortical granule release, causing swelling and hardening of the zona pellucida.
Phase 5 of Fertilization
- The female nucleus completes meiosis II.
- A second polar body is formed.
- Two pronuclei fuse.
Cleavage
- Cleavage increases cell number within the zona pellucida without increasing the size of the zygote.
- Cleavage begins around 30 hours after fertilization.
- Mammalian cleavage takes several days.
- Cleavage leads to blastomeres and a morula, which forms a blastocyst in around 3 days.
Compaction
- Blastomeres become tightly compacted, increasing cell-to-cell contact.
Blastocyst formation
- Blastomeres move towards one side of the embryo.
- A cavity called the blastocoele forms within the blastocyst.
- The embryo is composed of an outer trophoblast layer and an inner cell mass (embryoblast), and the zona pellucida is still present.
Early Pregnancy Factor (EPF)
- EPF is produced by the trophoblast.
- It is believed to help avoid a mother's immune responses.
Blastocyst Polarity
- Blastocysts have a polar side (the inner cell mass) and an opposite (mural), abembryonic side, where the trophoblast covers the surface..
Implantation
- The blastocyst implants in a prepared uterine environment at the end of the previous menstrual cycle.
- Three stages:
- Apposition (approaches the endometrium)
- Adhesion (close contact with adhesion molecules)
- Invasion (trophoblasts erode the endometrium)
Zygote, embryonic cells and chromosomal issues
- The zygote initially depends on maternal genetic material.
- The embryo generates its own genetic material during blastula.
- Mishap in chromosomal division during cleavage can lead to mosaicism.
Implantation Window
- A specific timeframe when the endometrium is receptive to embryo implantation.
- Implantation begins 4-6 days after the LH surge.
Invasive Trophoblast
- Syncytiotrophoblast: erosive and invasive.
- It digests endometrium for implantation passageway
Maintenance of Pregnancy
- Corpus luteum: produces progesterone, regulating endometrial function and trophoblastic activity.
- hCG (human chorionic gonadotropin): maintains the corpus luteum.
- Decidual reaction in the endometrial stroma protects the embryo.
Ectopic Pregnancy
- Implantation occurs outside of the uterine cavity.
- Commonly in the uterine tubes.
Second Week
- The embryo rearranges and forms an epiblast and hypoblast layers.
- Amniotic and yolk sac development.
Implantation Sites
- The typical implantation site is the uterine posterior wall.
- This is not the only implantation site; other locations are possible, but they can lead to complications.
Pregnancy Loss (Miscarriage)
- Spontaneous abortions frequently occur during the first 12 weeks of pregnancy.
- Reasons for miscarriage are multiple, including chromosomal abnormalities or maternal and placental or fetal causes.
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