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11 Questions

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Extracting DNA from Bananas

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Questions and Answers

What is the name of the molecule that carries genetic information?

Deoxyribonucleic acid (DNA)

What are the components of DNA?

Minerals, vitamins, and water

Sugars, phosphates, and nitrogenous bases (correct)

Proteins, carbohydrates, and lipids

Hormones, enzymes, and antibodies

What is the shape of DNA?

Single helix

Double helix (correct)

Circular

Linear

What is the purpose of the soap solution in the DNA extraction process?

To dissolve the cell membrane Signup and view all the answers

What is the role of the salt in the DNA extraction process?

To precipitate the DNA Signup and view all the answers

What is the role of the alcohol in the DNA extraction process?

To precipitate the DNA Signup and view all the answers

What is the name of the plant used in this experiment?

Musa paradisiaca Linn. (Saba) Signup and view all the answers

What is the purpose of blending the banana in the experiment?

To break down the banana cells into smaller pieces and release the DNA Signup and view all the answers

What is the purpose of filtering the mixture after adding the soap solution?

To remove the cell debris and other unwanted materials Signup and view all the answers

What is the purpose of adding alcohol to the mixture?

To precipitate the DNA out of the solution Signup and view all the answers

Why is it necessary to use a dropper to mix the filtrate gently with the alcohol?

To avoid disrupting the DNA strands and to allow for proper precipitation Signup and view all the answers

Study Notes

Objective

Students should extract DNA from bananas
Students should learn about genetically modified organisms
Students should write a position paper

Principle

DNA is gently separated from other cellular components
The Marmur procedure is used
Soap solution breaks down fats and proteins
Membranes surrounding cells are broken down
Salt causes the DNA strands to aggregate
Alcohol separates DNA and other components

Materials

100 mL graduated cylinder
10 mL graduated cylinder
250 mL beaker
Test tube (16 x 150)
Stirring rod
Test tube rack
Dropper
15 mL Ethyl Alcohol
150 mL Distilled Water
5 mL Joy dish washing liquid
1 g Sodium Chloride
2 Plastic Cups
2 Plastic Spoons
Ice cubes/tubes
Blender
Small cheese cloth

Procedure

Blend 1 peeled banana with 150 mL water
Mix 1 g salt with 10 mL water until dissolved
Add 5 mL dish soap to the mixture
Add banana mixture to soap solution and stir
Filter the mixture
Add 10 mL alcohol to a beaker with ice
Add filtrate to alcohol using a dropper
Gently mix and let stand
The glass rod is used to spool out the DNA

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Description

In this quiz, students will explore the process of extracting DNA from bananas using the Marmur procedure. Students will learn about the role of various materials and solutions in breaking down cellular components and understand the implications of genetically modified organisms. Additionally, participants will be tasked with writing a position paper related to this field of study.